3rd Africa Rice Congress Theme 1: climate resilient rice Mini symposium: Enhancing rice yield potential Author: Yuqing He

1. Gene Pyramiding to Improve Green Super
Rice by Molecular Marker-assisted Selection
Yuqing He
National Key Laboratory of Crop Genetic Improvement,
National Center of Crop Molecular Breeding
Huazhong Agricultural University
Wuhan, China
Email: yqhe@mail.hzau.edu.cn

2. The Problems of hybrid rice in China
• Lose of disease resistance (blast, bacterial
blight)
• Susceptible to insect pest (yellow stem borer
and brown planthopper)
• Cooking and eating quality
• Ever increasing demands for increase yield

3. Strategies for developing Green Super Rice
Green Super Rice should possess resistances to
multiple insects and diseases, high nutrient
efficiency, and drought resistance, promising
to greatly reduce the consumption of
pesticides, chemical fertilizers, and water.
Zhang, PNAS, 2007, 16, 104 (42): 16402–16409

4. Bt gene to improve hybrid rice with stem
borer and leaffolder resistance
Tu, J.et al. Nature Biotech, 2000,18:1101-1104

5. Bacterial Blight resistance to improve hybrid rice
Chr. 11
Chr. 6
G1465
2.0
1.5
RG1109
AFLP1415
C50
4.0
2.8
1.0
0.8
0.8
3.0
C133
C1003
C189
RG103
21
248
Xa7
M5
0.9
Xa21
Xa23
STSP3
AB9
3.0
Marker from IRRI
CDO534
Chen et al. Crop Science, 2000, 40: 239-244
Zhang et al，Plant Breed，2006，125, 600-605

6. Comparison of bacterial resistance to GX325 and KS-1-21 as shown by lesion length
among nine genotypes of an F2 population using Tukey-Kramer HSD test
Genotype
Genes
No.
xa21xa21xa7xa7
0
xa21xa21Xa7xa7
1
Xa21xa21xa7xa7
1
Xa21Xa21xa7xa7
2
D
4.84
xa21xa21Xa7Xa7
2
D
4.31
Xa21Xa21Xa7xa7
3
E
2.67
Xa21xa21Xa7xa7
2
E
Xa21xa21Xa7Xa7
3
Xa21Xa21Xa7Xa7
4
GX325
KS-1-21
Mean
A
Mean
19.58 A
B
12.38
C
6.47
22.071
B
4.20
C
7.96
D
5.05
C
7.16
D
E
3.64
2.31
E
3.33
E
2.10
E
3.09
E
1.61
E
2.23

7. MH63(Xa21) and MH63(CK)

8. The locations of Pi-1 and Pi-2
Chr 6
Chr 11
4.5
5.1
5.1
2.2
6.8
9.7
Y2668LA
Y6854L
R251
RM224
R543a
C235
0.4
RG64
0.9
1.2
Pi-2 Pi9, Pigm, R6
AP22
0.3
RG445
Pi-1
RZ536
BC1F2 of
Zhenshan 97/BL5
1.5
RZ632(R2123)
F5 (CO39/C101A51)
RILs

9. Reaction of Pyricularia grisea isolates on the differential
near isogenic lines (NILs) (Liu et al, 2003)
NILs
gene
C101LAC
C101A51
C104PKT
BL1
BL2
BL5
BL3
BL4
BL6
CO39（ck）
Zhenshan97
Pi1
Pi2
Pi3
Pi1+Pi3
Pi1+Pi3
Pi1+Pi3
Pi2+Pi3
Pi2+Pi3
Pi1+Pi2+Pi3
no
unknown
Infection
strains
13
11
57
8
7
5
4
5
2
75
46
Resistance
frequency (%)
82.7
85.3
24.0
89.3
90.7
93.3
94.7
93.3
97.3
0
38.7

10. Blast natural identify area
Yuan’an, Hubei
(Altitude:550m)
Enshi, Hubei
（ Altitude:600m）

11. Fig. 2 The distribution of blast
resistance scores of the improved
versions of Jin 23B and their hybrids
compared with control varieties. a the
leaf blast resistance scores of the
improved Jin 23B, hybrids, and control
at tillering stage. b the leaf blast
resistance scores of the improved Jin
23B, hybrids and control at heading
stage. c the neck blast resistance scores
of the improved Jin 23B, hybrids and
control at maturity stage.
Jiang et al, Mol Breed: 2012

12. BPH resistance genes
Chr 4b
Chr 4a
Chr 3
Chr 12
RM307
2.2
RM514
RM8213
RM16434
Bph11(t)
RM313
Bph12
bph2
Bph10
20.6
15.0
12.5
Bph17
19.1
SG1
2.9
HJ22
RM16717
Bph24
RM463
9.0
5.2
SG6
8.0
RM261
QBph3
RM570
3.6
HJ16
0.7
2.2
10.0
IN762
7.0
Bph14
10.1
J14-12
J23/MS5
QBph4.2
RM16820
6.1
RM16853
Bph27
J18-4
IN187
4.2
HJ12
4
HJ28
J14-8
7.0
2.1
IN156
J18-15
2
Bph15
QBph4.1
0.2
29.3
6.6
Bph20
11.5
4.7
1.0
RM5953
bph16
21.2
0.9
J6-4
RM119
J6-10
26.9
Hu et al, Mol Breed (2012) 29:61–69
Hu et al, PMS, Pest Manag Sci (2013) 69: 802–808
RM252
Bph6
Bph18
Bph9
RM3331
HJ9
RM28625
RM270
Bph21
Bph1

13. Hybrids
TN1
Hu7(18)
B5(14/15)
Y58S/9311
Y58S/9311(18)
Y58S/9311(15)
Y58S/9311(14)
Y58S/9311(15/18)
Y58S/9311(14/18)
Y58S/9311(14/15)
Y58S/9311(14/15/18)
GZ63S/9311
GZ63S/9311(18)
GZ63S/9311(15)
GZ63S/9311(14)
GZ63S/9311(15/18)
GZ63S/9311(14/18)
GZ63S/9311(14/15)
GZ63S/9311(14/15/18)
6303S/9311
6303S/9311(18)
6303S/9311(15)
6303S/9311(14)
6303S/9311(15/18)
6303S/9311(14/18)
6303S/9311(14/15)
6303S/9311(14/15/18)
Resistance Score
The resistance score of pyramided two-line hybrids
9
8
7
6
5
4
3
2
1
0

14. 21 days

15. BPH resistance in field experiment (4 hectares)
(2010, Tongcheng, Hubei)
GZ63S/9311(ck)
GZ63S/9311(Bph14/Bph15)

16. GZ63S/9311(ck)
GZ63S/9311(Bph14/Bph15)

17. Whole genome selection breeding
R6: blast resistance gene
60K SNPs Background selection

18. Kongyu 131(Pi2)
Pi2

19. Acknowledgement
Ph D and postgraduate:
Hu Jie
Yuan Li
Gao Guanjun
Tu Juming
Chen Sheng
Jiang Gonghao
Zhang Jinfeng
Yang Zixian
Li Xin
Liu Shiping
Wu Changjun
Staff
Rice group
Collaboration:
IRRI
SAGC
CAAS
Wuhan University
Cornell University
Supported: 863 plan; National Natural Science Foundation
in China and Bill and Melinda Gates Foundation

20. Thank You!