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Massons Trichrome Stain
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Massons Trichrome Stain
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MASSONS TRICHROME STAIN
PRESENTED BY: MEHTAB SHAH B.S MT 3 Rd year (SDLS(LNH) HISTOPATHOLOGY
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FIXATIVE
Bouin’s solution is preferred 10% neutral buffered formalin may be used TECHNIQUE Cut paraffin section at 4 microns
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Light green solution
2-5mint; Dehydrate in absolute alcohol Clear in xylene Mount in synthetic resin Microscopy
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Nuclei red, collagen
green, cytoplasm red
20.
Infracted rat heart
showing damaged cardiac tissue in blue.
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Terminal ileum with
increase in subepithelial collagen table in a patient with collagenous colitis
22.
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