Toxicity evaluation of pyridone derivatives on Sytophilus oryzae

1. M.V.P.Samaj’s
K.T.H.M. COLLEGE,
GANGAPUR ROAD, NASHIK – 422 002
DEPARTMENT OF ZOOLOGY
WELCOMES

2. TOXICITY EVALUATION OF PYRIDONE
DERIVATIVES ON Sitophilus Oryzae Linn

3. By
Mrs. Jayshree Vitthal Shinde
Under the guidance of
Dr. A. E. Desai

4. INTRODUCTION
• Insect existed at least 20 million yrs ago.
• Grain pests e.g. Rodents, Nematodes, Fungi
• Classification of pests
• Plants extracts/ botanical insecticides
• Synthetic chemical compounds

5. Aim Of This Project
• Toxicity Evaluation of
Synthesized Chemical
Compounds on Stored Grain
Pests.
• To Protect Stored Grains.

6. Material & Methods
• Collection & Culture Of Sitophilus oryzae
Linn
• Synthesis of chemical compounds.

7. Observation Table
Table no 1:
2,5dihydro-7,8dimethoxy-4(3-methoxyphenyl)-2-oxo-1H-in
deno (1,2-b)pyridine-3carbonitrile.
Sr.no. Dose No.of Mortality
in insects for
ppm released 6hrs 12hrs 18hrs 24hrs
1 5 10 1 2 3 6
2 10 10 2 3 3 7
3 15 10 2 3 4 8
4 20 10 3 3 4 9

8. Table no 2:
2,5dihydro-7,8dimethoxy-4(3,4-dimethoxyphenyl)-2-
oxo-1H-in deno (1,2-b)pyridine-3carbonitrile.
Sr.no. Dose No.of Mortality
in insects for
ppm released 6hrs 12hrs 18hrs 24hrs
1 5 10 2 2 2 5
2 10 10 2 3 3 6
3 15 10 3 3 3 8
4 20 10 2 3 4 9

9. Table no 3:
2,5dihydro-7,8dimethoxy-4(3,4,5-trimethoxyphenyl)-2-oxo-1H-
in deno (1,2-b)pyridine-3carbonitrile.
Sr.no. Dose No.of Mortality
in insects for
ppm released 6hrs 12hrs 18hrs 24hrs
1 5 10 1 2 3 6
2 10 10 2 3 3 8
3 15 10 2 3 4 9
4 20 10 3 3 3 9

10. Table no 4:
2,5dihydro-7,8dimethoxy-4(thiophen-2-yl)oxo-1H-in deno
(1,2-b)pyridine-3carbonitrile.
Sr.no. Dose No.of Mortality
in insects for
ppm released 6hrs 12hrs 18hrs 24hrs
1 5 10 2 2 3 6
2 10 10 2 3 3 7
3 15 10 2 3 4 9
4 20 10 3 2 3 9

11. Result
Table No. 9 - Comparative Results
Chemical
Time in Mortality LC50 ppm
Regression eqn
Heterogencity
VarianceFinancial
2
Comp Hrs. ± S.E. Y-a+b x (n-1) Limit
0 5 10 15 20 A=y-bx
Comp I 24 0 60 70 80 90 1.5515 ± 0.9982
4.438 2.46 0.997 m1=0.0951
(101) m2=3.0075
Comp 2 24 0 50 60 80 90 1.3357 ± 0.7354 -2.539
3.908 0.54 m1=0.1044
(104) m2=2.567
Comp 3 24 0 60 80 90 90 1.5885 ± 0.9567 2.983
4.447 0.905 m1=0.1034
(105) m2=3.0736
Comp 4 24 0 60 70 90 90 1.5134 ± 0.8654 2.936
4.315 0.802 m1=0.0311
(106) m2=2.9957

12. Graph

13. Discussion
• There is significant increase in mortality from low
to high dose of chemical compound.
• S. oryzae, R. dominica, T. castaneum shows high
mortality when exposed to biogass in PVC bins
(Bennett, 2003).
• Azadirachtin exhibited good efficiency against
Musca nobulo L, Culex fatigans, Tribolium
castaneum (Paul et, al.1963).
• PN101, LC50=1.5515+0.9982, PN104,
LC50=1.3357+0.7354, PN105,LC50=1.5134+0.8954,
PN106, LC50=1.5885+0.9567.

14. References
• Bennett S. (2003). Stored Product insects,
available on: www.thepiedpiper.co.uk/th7.htm.
(Accessed 22march 2012)
• Finney D.(1971). Probit analysis Cambridge
University press, London.
• Paul, C.F, R.S.Dixit and P.N. Agarwal. (1963).
Evaluation of the insecticidal properties of the
seed oil and leaf extract of the common Indian
neem, Azadirachta indica L. Sci. Cult. 29:412-413