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Biotech 2011-08-recombinant-dna

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Nikolay Vyahhi
Nikolay Vyahhi
Technologie
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Cloning Overview ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Libraries ,[object Object],[object Object],[object Object],[object Object]
Three general types of libraries ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Genomic Library ,[object Object],[object Object],[object Object],[object Object],[object Object]
cDNA Libraries ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Expression libraries ,[object Object],[object Object],[object Object],[object Object],[object Object]
Why clone? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Cloning reagents ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
DNA ligase ,[object Object],[object Object],[object Object],[object Object],[object Object]
Reverse transcriptase ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Vectors ,[object Object],[object Object],[object Object],[object Object],[object Object]
PBR322 ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The host ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Colony screens ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
What to do with the DNA? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Examples of medical relevance ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Cloning insulin ,[object Object],[object Object],[object Object],[object Object]
cDNA clones were necessary ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Identifying the insulin cDNA clone ,[object Object],[object Object],[object Object],[object Object]
Expressing the clone ,[object Object],[object Object],[object Object],[object Object]
Problems with expressing eukaryotic genes in a bacterium ,[object Object],[object Object],[object Object]
Another approach ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Reconstitution of insulin from individual chains ,[object Object],[object Object],[object Object],[object Object]
Present day ,[object Object],[object Object],[object Object],[object Object]
Gene therapy for IDDM type I? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Beta cell regulation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Other human diseases potentially amenable to gene therapy ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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Biotech 2011-08-recombinant-dna

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