8. LKB1 Fyn Merge DAPI c) b) a) d) f) e) g) h) Kinase inactive Fyn recruits LKB1 out of the 3T3L1 adipocyte nucleus Fyn-CA (Y528F) Fyn-KD (K299M) Fyn-CA % Nuclear LKB1 100 80 60 40 20 0 Fyn-KD
9. LKB1 Y261 and Y365 are required for nuclear localization LKB1 DAPI Merge LKB1- WT a) b) c) LKB1- Y60F d) e) f) LKB1- Y365F d) e) f) LKB1- Y261/365F g) h) i) LKB1- Y261F a) b) c) LKB1 DAPI Merge
10. LKB1 Y261/365F increases AMPK phosphorylation Endo-AMPK P (T172) -AMPK Flag LKB1- WT LKB1- Y261/365F B) SKM GST-AMPK Flag LKB1- WT LKB1- Y261/365F A) HeLa P (T172) -AMPK
12. LKB1 Y261/365F but not P328A prevents Fyn- CA induced nuclear localization DAPI Merge Fyn-CA LKB1- Y261/365F LKB1-P328A DAPI Merge Fyn-CA a) c) d) b) e) g) h) f)