9. Preparando ADN Recombinante 5’ 3’ G C T T A A A A T T C G G A A T T C C T T A A G 3’ 5’ one DNA fragment another DNA fragment 3’ 5’ In-text figure Page 254
10. nick 5’ 3’ 3’ 5’ G A A T T C C T T A A G nick G A A T T C C T T A A G DNA ligase action In-text figure Page 254
17. Polymerase Chain Reaction Double-stranded DNA to copy Figure 16.6 Page 256 Stepped Art DNA heated to 90°– 94°C Primers added to base-pair with ends Mixture cooled; base-pairing of primers and ends of DNA strands DNA polymerases assemble new DNA strands
18. Polymerase Chain Reaction Figure 16.6 Page 256 Stepped Art Mixture heated again; makes all DNA fragments unwind Mixture cooled; base-pairing between primers and ends of single DNA strands DNA polymerase action again doubles number of identical DNA fragments
34. Uso de una sonda Colonies on plate Cells adhere to filter Cells are lysed; DNA sticks to filter Probe is added Location where probe binds forms dark spot on film, indicates colony with gene Figure 16.9 Page 259
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43. Anormalidades cromosómicas CLONES Enfermedades ADNc ADNg Familias Ligamiento Híbridos celulares FISH Clones contiguos etc. MAPA DEL GENOMA HUMANO Mapa físico Mapa genético Secuenciación Identificación de genes Mapa RATON P o l i m o r f i s m o s
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