43. Putting PowerPoint to Work for You For entering students N = 36 in 2006 N = 33 in 2007 It took centuries to decipher the Rosetta stone… Your audience has 1 minute…
44. Putting PowerPoint to Work for You Good slide vs. poor slide… Sometimes… the difference is subtle…
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49. Putting PowerPoint to Work for You It a scary world out there… In our lecture halls…
50. Neuromuscular Junction ( Motor Neuron to Muscle Fiber Synapse) Freeze fracture cleaves membranes in hydrophobic plane of bilayer Electron micrograph
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52. Sphingolipid and cholesterol cause distinct phase to form in pure lipid mixtures containing phospholipid with saturated fatty acid chains Mix DOPC (unsat’d) or DPPC (sat’d) at different ratios, with sphingolipid +/- cholesterol, at 23 o C or 37 o C. Assay by fluorescence quenching of marker that only sees sol phase lipid. Phase separation indicated by break in slope of quenching curves. Unsaturated fatty acids inhibit 2nd phase. See 2 nd phase at 23 o C with increasing DPPC . Need cholesterol to obtain 2nd phase at 37 o C. Cholesterol promotes 2 nd phase formation
53. Human Genome Project 1990-2003 HAPMAP 2002 – http://www.hapmap.org/ ENCODE Project 2003 - ENCyclopedia of DNA Elements : identify all functional elements in the human genome sequence compare the genetic sequences of different individuals to identify chromosomal regions where genetic variants are shared.
54. 3: Examine Region Chromosome-wide summary data is shown in overview Default tracks show HapMap genotyped SNPs, named genes from Entrez, and alternative mRNA splicing patterns
55. Electrical activation of skeletal muscle force production Single stimulus, single twitch Multiple stimuli, unfused or fused tetanus Prolonged depolarization, long contracture then relax ( Not physiological ) Forc e Forc e Forc e AP Time
58. high voltage sample m 1 m 2 m 3 detector drift region laser Matrix-assisted Laser Desorption/Ionization (MALDI) Time-of-Flight (TOF) Analyzer MALDI v 1 m 1 v 2 m 2 v 3 m 3
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60. in situ immuno How do we experimentally identify a tissue or cell type? 1. Histology smooth muscle nerve cells