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Flow Cytometry TEACHER GUIDE SHEET Click to view POWERPOINT ACTIVITY
What cells are in there? How   can we use Flow Cytometry to quantify cells?
[object Object],[object Object],[object Object],[object Object],[object Object]
Applications ,[object Object],[object Object],Dr. Allan MCB Harvard
White Blood Cell Differentials ,[object Object],[object Object],[object Object],[object Object],[object Object],White Blood Cells, Platelets (stained purple), a T-Lymphocyte white cell (stained green), and a Monocyte white cell (stained gold) as seen through a scanning electron microscope. ©2000 Dennis Kunkel, Ph.D. Image Source: http://www.mybloodyourblood.org/biology_white.htm
How could you separate your sample of white blood cells into subpopulations? What characteristics of the cell could you use? How about if they had no color?
Standard Differentials 1 2 3 ,[object Object],[object Object],[object Object],[object Object],http://www. rnceus .com/ cbc / cbcwbc .html
Why do we need technology?
Flow Cytometry How it works 1. Draw cells, with excess fluid, from test tube into machine. 2. Cells pass in single file past laser. 3. Laser hits cell and light is scattered. 4. Photomultiplier multiplies light intensity and a light sensor measures the amount of light and scatter pattern. 5. Based on cell characteristics (size and shape), the computer categorizes and quantifies the cells. Click to learn more
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Action
Use the following key to categorize your sample of White Blood Cells. What is the percent break up of your white blood cell differential? Action Lymphocyte White Nerds Eosinophil Pink Runt Basophil Yellow Runt Monocyte Green Runt Neutrophil Orange Runt Cell Candy
What can this differential tell you? http://www. bioteach . ubc .ca/ MolecularBiology / FlowCytometry / See real Data
      Let’s take a closer look. How can we differentiate T cells? http://www.flow- cytometry .de/ Fluorescent Markers
Create a table to categorize and count  your T-cell subpopulation into CD4+ Helper T cells and CD8+ Cytotoxic T cells. Purple Nerds= CD8+ Cytotoxic T cells Pink Nerds += CD4+ Helper T cells Categorize and count your T cell population Action
What can the results tell you? See real Data
Homework What other applications are there for the technology of flow cytometry? Use the internet to explain 1 other application of the flow cytometer. E sure to document your resource.
http://courses.dce.harvard.edu/-bios169c/resourcemain.html  maintained by Dr. Jeffrey Lyczak Resources

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Flow cytometrypresentation

  • 1. Flow Cytometry TEACHER GUIDE SHEET Click to view POWERPOINT ACTIVITY
  • 2. What cells are in there? How can we use Flow Cytometry to quantify cells?
  • 3.
  • 4.
  • 5.
  • 6. How could you separate your sample of white blood cells into subpopulations? What characteristics of the cell could you use? How about if they had no color?
  • 7.
  • 8. Why do we need technology?
  • 9. Flow Cytometry How it works 1. Draw cells, with excess fluid, from test tube into machine. 2. Cells pass in single file past laser. 3. Laser hits cell and light is scattered. 4. Photomultiplier multiplies light intensity and a light sensor measures the amount of light and scatter pattern. 5. Based on cell characteristics (size and shape), the computer categorizes and quantifies the cells. Click to learn more
  • 10.
  • 11. Use the following key to categorize your sample of White Blood Cells. What is the percent break up of your white blood cell differential? Action Lymphocyte White Nerds Eosinophil Pink Runt Basophil Yellow Runt Monocyte Green Runt Neutrophil Orange Runt Cell Candy
  • 12. What can this differential tell you? http://www. bioteach . ubc .ca/ MolecularBiology / FlowCytometry / See real Data
  • 13. Let’s take a closer look. How can we differentiate T cells? http://www.flow- cytometry .de/ Fluorescent Markers
  • 14. Create a table to categorize and count your T-cell subpopulation into CD4+ Helper T cells and CD8+ Cytotoxic T cells. Purple Nerds= CD8+ Cytotoxic T cells Pink Nerds += CD4+ Helper T cells Categorize and count your T cell population Action
  • 15. What can the results tell you? See real Data
  • 16. Homework What other applications are there for the technology of flow cytometry? Use the internet to explain 1 other application of the flow cytometer. E sure to document your resource.