This document describes three experiments where genes involved in DNA mismatch repair were knocked down in cells. Knocking down MLH1 or MSH2 did not significantly shorten cell lifespan with p-values above 0.05. However, knocking down EXO1 did significantly shorten lifespan with a p-value of 0.0388. Overall lifespan was not shortened when the genes were knocked down and the DNA was damaged with gamma rays.

5. Materials and Methods

6. ⑴ MLH1 knockdown
p=０．４４５２

7. ⑴ MLH1 knockdown
p=０．４４５２

8. ⑵ MSH2 knockdown
p=０．２３５１

9. ⑵ MSH2 knockdown
p=０．２３５１

10. ⑶ EXO1 knockdown
p=０．０３８８

11. ⑶ EXO1 knockdown
p=０．０３８８

12. Life span was not be shortened
by MLH1/MSH2/EXO1-knockdown
(DNA was damaged by γ-rays)