Dna fingerprinting

What is DNA fingerprinting ? ,[object Object]

A Historical Perspective ,[object Object],[object Object],[object Object]

DNA Fingerprinting & DNA Profiling - same or different ? ,[object Object],[object Object]

Stages of DNA Profiling ,[object Object],[object Object],[object Object],[object Object]

Stages of DNA Profiling ,[object Object],[object Object],[object Object]

Stages of DNA Profiling ,[object Object],[object Object]

Uses of DNA Profiling ,[object Object]

Crime ,[object Object],[object Object],[object Object]

Biological materials used for DNA profiling ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

DNA Profiling can solve crimes ,[object Object],[object Object],[object Object]

Example ,[object Object],[object Object],[object Object]

Was the suspect at the crime scene? Suspects Profile Blood sample from crime scene Victims profile

Solving Medical Problems ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

An Example Using EcoR I for a Question of Paternity ,[object Object],[object Object],[object Object],Recall: EcoR I cuts only at GAATTC

Example Con’t ,[object Object],[object Object],[object Object],[object Object]

Resulting Picture after Electrophoresis ,[object Object]

Paternity Test ,[object Object],[object Object]

What is Analyzed in the DNA ? ,[object Object],[object Object],[object Object]

Short Tandem Repeats (STRs) the repeat region is variable between samples while the flanking regions where PCR primers bind are constant 7 repeats 8 repeats Homozygote = both alleles are the same length Heterozygote = alleles differ and can be resolved from one another AATG

An Example of a STR in locus D7S280 ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

New Technology ,[object Object],[object Object]

Some uses of DNA Profiling ,[object Object],[object Object],[object Object],[object Object],[object Object]

Parentage Testing as conducted at DNA Diagnostics, Auckland

Why Test ? ,[object Object],[object Object],[object Object],[object Object]

Why Test? ctd ,[object Object],[object Object],[object Object]

The Steps ,[object Object],[object Object],[object Object]

The Steps, II ,[object Object],[object Object]

The Steps, III ,[object Object]

The Steps, IV ,[object Object],[object Object]

The Steps, IV, ctd ,[object Object],[object Object]

The Steps, V ,[object Object],[object Object]

The Steps, VI ,[object Object],[object Object],[object Object],[object Object]

An ABI Prism 310 Genetic Analyser Capillary tube Sample tray Note-other models of this Analyzer have more capillary tubes and can process more samples at a time, but this model is sufficient for the demand for testing to date through DNA Diagnostics

Analyzing the Read-out ,[object Object],[object Object],[object Object],Locus D19S433 = 14,15 Locus vWA = 15,16 Locus TPOX= 8,8 Locus D18S51= 13,16 A sample showing 4 loci- The top line is a ‘ladder’ for comparison

A sample print -out for one person, showing all loci tested. Different colours help with interpretation

Whose STR? ,[object Object],[object Object],[object Object],[object Object]

Parentage Analysis ,[object Object],[object Object],[object Object]

Parentage Analysis II, ctd Paternity index The index in this man’s analysis shows that the DNA evidence is 25 million times more likely that he is the biological father versus he is not (odds 25 million:1)

Finally ,[object Object],[object Object]

Dna fingerprinting

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Dna fingerprinting