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Presenting NuLiv Science International July, 2010
NuLiv Operates on 6 Interconnected Business Platforms ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Focused Research on Metabolism For close to a decade, NuLiv has centered its research around metabolism, the biological functions that sustain life, at cellular and systemic levels 1. Research initiatives (slides 4 – 6) 1.1 Central belief  1.2 Hypothesis 1.3 Strategy 2. Life Science and Clinical Research platforms (slides 7 –14) 2.1 Facility 2.2 Staff & advisors 2.3 Validation platform 2.4 Supporting framework 3. Patents (slide 15) 4. Papers (slide 16)
The Central Belief that laid down  the Foundation for Our Research ,[object Object],[object Object],[object Object],[object Object],[object Object]
The Hypothesis Our Research Strategy is Built Upon ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Our Strategy is to Build a Research Platform that Allow us to Discover Novel Bioactive Compounds from Chinese “Qi” and “Xue” Herbs ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
NuLiv’s Advanced and Integrated  Life Science and Clinical Research Platform   Component Description Facility NuLiv Taipei Laboratory NuLiv Shanghai Laboratory Management, Staff and Advisors Highly educated and trained in business, multi scientific & clinical disciplines, and work closely in a truly interdisciplinary manner Discovery and Validation In-house and 3 rd  party in vitro, in vivo, and human use study platforms  Supporting Framework/Network Supported by NuLiv’s own subsidiaries and affiliates, and long term strategic partners and alliances in many distinguished academic and research institutions
Research Facilities Taipei and Shanghai Laboratories ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
NuLiv Taipei Laboratory Has Access to Equipments to Perform a Wide Range of In Vitro and In Vivo Studies Laminar Flow, Cell Culture Water Purification System CO2 Incubator Lab Bench Spectrophotometer Photomicrographic Equipment High Speed Centrifuge PCR machine Gel Dryer Deep Fryer Gel Photosystem Animal Room National Defense Medical Center
[object Object],Extraction/Separation /Isolation Room Chemical Analysis Room Extraction Equipments Waters 2695 HPLC Agilent 1100 HPLC Agilent 6890N/5973 GC/MS Camag TLCS Shimadzu 2401PC UV spectrophotometer Nikon YS2   biological microscope Instrumental Analysis Room
NuLiv Management /Business & Research Executives Founder & CEO Austin Wang, M.S. NuLiv Holdings, Inc. (World Headquarters) Taipei City, Taiwan Executive Director Y.H. Lee, M.D. Vice Chancellor, China Medical Univ. (Taiwan), Previously Dean, Chang Kung Medical School, Dep. Director,  Chang Kung Hospital System (10,000+ beds)  Principle Scientific Advisor  H.C. Lin, Ph.D. Univ. of  North Carolina. Taught TCM at NDMC for over 20 years. Pioneered the hypothesis that “Qi” herbs regulate absorption and other metabolic functions President Americas, Europe, Asia, Africa Business Development Sales & Marketing Representative Americas, Europe, Asia, Africa Michael Wang, M.S. NuLiv Science USA, Walnut, CA David Allen, Ph.D. World Wellness, LLC., San Diego, CA  President Greater China David Wu, Ph.D. Business Administration, Univ. of South Australia,  Director Research & Development NuLiv Taipei Laboratory Thomas Yang, Ph.D., Molecular Microbiology, Univ. of Texas, Southwestern Medical Center Director NuLiv Shanghai Laboratory Sunnie Sun, Ph.D., Pharm. Analysis, Sheng Yang Pharm. Univ. Director Clinical Research Rose Chen, M.D., Ph.D., Shanghai TCM University
NuLiv’s Scientific Advisors Chang, T.C., Ph.D. Biochemistry, Univ. of Illinois, Professor, Dept. of Biochemistry, National Defense Medical Center,Taiwan Chang, W.L., Ph.D. Pharmacy, National Taiwan Univ., Professor, School. of Pharmacy, National Defense Medical Center, Taiwan Kuo, C.H., Ph.D. Physiokinetics, Univ. of Texas, Professor and Chair, Department of Exercise Science, National Taipei Physical Education College, Taiwan Chen, C.X., Professor Director, Department of Pharmacology, TCM Univ., Shanghai, China.  Serves as our toxicity and safety advisor on TCM and  other herbs Komatsu, Yasuhiro, Ph.D. Medical science, Juntendo University, Prior Dean of R&D department, Tsumura & Co.  Chu, C. H, Ph.D. Food engineering, University of Tokyo, expert in pre-clinical pharmacology tests Fan, David, Ph.D. Senior Lecturer, Trinity College, Cambridge Univ., Editor-in-chief, Angiogenesis Hsieh, Steve, Ph.D. Nutritional Biochemistry, Univ. of Wisconsin, Ex Vice Chairman, National Science Council, R.O.C. Government  Looi Hwang, Ph.D. Industrial & Environmental Pharmacy Ph.D. from Purdue, previously G.M. of MSD, Hanzhou, China & and Merck of Singapore J.K. Luo, Ph.D. Editor (acting), Journal of Heterocyclic Chemistry, Professor at University of S. Florida Funfack, Wolf, M.D. Metabolic Balance founder.  Peng, C.K., Ph.D. Statistical Physics, Boston Univ., Assoc. Professor/Co-director, Rey Institute for Nonlinear Dynamics in Physiology and Medicine, Harvard Medical School.
Our In vitro, In vivo, and Human Research Platforms In vitro Assay Systems ,[object Object],[object Object],[object Object],In vivo Assay for efficacy and safety  Systems ,[object Object],[object Object],[object Object],[object Object],[object Object],Human use Study ,[object Object],[object Object],[object Object]
Our Supporting Framework & Network Facility, equipment, interdisciplinary consultation National Defense Medical Center Industrial Technology Research institute, Taiwan Yang Ming University, Taiwan Taipei Medical University, Taiwan In vivo  and human study China Pharmaceutical University, Nanjing, China Taipei Physical Education College I-Shou University Hospital NuLiv Wellness Clinic Consultation, Cellular science Metabolic health TCM pharmacology &  toxicology angiogenesis Yang Ming Univ. Stem Cell Research Center Taipei Medical Univ. Stem Cell Research Center Dr. Wolfgang Funfack, Metabolic Balance, De.  Shanghai TCM university China Medical University, Taiwan Trinity college, Cambridge University, UK
Patents from NuLiv’s Research Title Country/Region  Patent NO. Application NO. Method for Regulating Nutrient Absorption with Astragalosides USA US11/426,029  China CN200610090113.0  Taiwan I271195  Method for Regulating Nutrient Absorption with Ginsenosides  USA US11/426,06  China CN200610090114.5  CN200910178493.7 CN200910178492.2 Taiwan I317280 Method for Skin Care PCT PCT/US07/77326 USA US11/469,659 China CN200710089586.3  Taiwan TW095132614  Use of Cycloartane compounds for treating arthritis USA US12/353,779 China  N/A yet Taiwan TW098135306
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Published Papers and papers in Press
Discovery of Novel, Safe, and Bioactive  Botanical Fractions and Compounds ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
6 Fractions / Compounds from our Research and Their Biological Functions Fractions/Compounds Discovered   Functions NL-F001 Anti-angiogeneis NL-F002 Enhance amino acids, vitamins, and glucose absorption at the cellular level; anti-angiogenesis NL-F003 Increase Adiponectin secretion Ergogenic/recovery NL-F004 Enhance amino acids, vitamins, and glucose absorption at the cellular level NL-F006 Enhance amino acids, vitamins, and glucose absorption at the cellular level ; Increase Adiponectin secretion NL-F007 Increase collagen and hyaluronic acid secretion; decrease MMP-1 secretion
[object Object],AstraGin TM An all natural nutrient uptake enhancing complex that improves amino acids, glucose, vitamins absorption by 62%, 57%, 50% through regulation and parallel alteration on both the protein and mRNA expression levels of the nutrient transporter genes Astrion TM Reduces the number and appearance of fine lines & wrinkles, skin discoloration due to sun damage, and firms, tones, and hydrates skin through increased dermal genesis and UV protection. Dermal genesis promotes the synthesis of new collagen and hyaluronic acid and, at the same time, reduces the breakdown of existing collagen due to UV light destruction ActiGin TM Increased delivery of glucose to muscle cells; Protect against free radical attack after exercise; Anti-inflammation; Minimize muscle cell damage; Increased glycogen  replenishment after Intensive workout ImmunoAG TM ImmunoAG TM  promotes nutrient absorption and metabolism in intestinal cells, Inhibits abnormal cell proliferation and angiogenesis, induces abnormal cell mitotic arrest and apoptosis through the activation of multiple signal pathways. In an  in vivo  study, ImmunoAG TM  demonstrated to inhibit abnormal cell growth by 44% and prolonged the life of subcutaneous abnormal cell bearing rats by 33% when compared to a control group. OsteoSine TM Promotes bone mineral density and structural strength by enhancing bone formation and reducing bone resorption. This dual action has been confirmed by NuLiv’s PO (post-ovariectomized) female rats ( equivalent to menopausal women) and  RA (retinoic Acid- induced acute osteoporosis) rats (Equivalent to none gender or age-related osteoporosis persons) models. Slim&Fit TM Decreases the intestinal absorption of glucose through the regulation and parallel alteration on both the protein and mRNA expression levels of the nutrient transporter genes while increases the glucose absorption in muscle and adipocyte cells through insulin independent pathway.
How ActiGin TM  Works Creatine  kinase MDA IL-6 Inflammatory biomarkers inhibition Exercise Performance Fast Recovery Exercise Oxidative stress elevation Glucose uptake Oxidative stress reduction ATP
[object Object],AstraGin TM ’s Nutrients Absorption Model
How Astrion TM  Works
Pharmacodynamics of ImmunoAG TM
[object Object],Normal bone treated with retinoic acid to simulate osteoporosis conditions Treated with 400 mg of OsteoSine daily for 5 weeks
[object Object],Slim&Fit  TM ’s Biological Pathways/Mechanisms on Regulating Metabolic Functions
Development of  Production Process  for  NuLiv’s Proprietary Bioactive Fractions and Compounds ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Extraction, Fractionation, Separation,  and Purification  of Raw Herbs to Fraction and Compound Levels
The Purity and Concentration Level of our Bioactive Fractions and Compounds are Identified and Measured by Specific Bioassays Active constituents Medicinal  plants Crude  extracts Fraction 1 Fraction 2 Fraction 3 Fraction 4 Fraction 5 Fraction 6 … fractionation extraction Formulation Depend on function Products & Marketing Structure  elucidation UV MS NMR IR CD 1 H NMR 13 C NMR 2D NMR separation Absorption Anti-cancer Skin care, etc Active constituents QC systems -LC/UV -GC/MS Bioassay QC
Our Supplements are Highly Effective and Safe in Supporting Critical Human Health Functions
Our Sourcing Operations Guarantees Premier Quality Products with Competitive Prices and Timely Delivery to our Customers NuLiv/ Customer specifications/requirements Vendor Vendor Survey for  Certificates, Qualifications, MOA, COA, sample Onsite Vendor Survey Disqualified Qualified Contract / Agreement Primary  suppliers Secondary Supplier
Our Quality Control on Sourcing  Operations Closely  Follows  Pharmaceutical Protocols ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Futuristic Wellness Product and Service Platform (A NuLiv Experimental Model)  Wellness Advisors Interactive and personal wellness information exchange  and virtual community management NuLiv Wellness Management Inc. Device HRM Meridian Blood analysis   CD/Biochips Nutritional Sciences NuLiv Laboratories (Slides 1 – 42) System complexity analysis DynaDx – Harvard Reylab Evidence based wellness know-how NuLiv Wellness Clinic Wellness/Sub-clinical Primary Care Clinic Cellular sciences Steminent Therapeutic Inc .
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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Nuliv an overview of_nu_liv_science_2010-6-21

  • 1. Presenting NuLiv Science International July, 2010
  • 2.
  • 3. Focused Research on Metabolism For close to a decade, NuLiv has centered its research around metabolism, the biological functions that sustain life, at cellular and systemic levels 1. Research initiatives (slides 4 – 6) 1.1 Central belief 1.2 Hypothesis 1.3 Strategy 2. Life Science and Clinical Research platforms (slides 7 –14) 2.1 Facility 2.2 Staff & advisors 2.3 Validation platform 2.4 Supporting framework 3. Patents (slide 15) 4. Papers (slide 16)
  • 4.
  • 5.
  • 6.
  • 7. NuLiv’s Advanced and Integrated Life Science and Clinical Research Platform Component Description Facility NuLiv Taipei Laboratory NuLiv Shanghai Laboratory Management, Staff and Advisors Highly educated and trained in business, multi scientific & clinical disciplines, and work closely in a truly interdisciplinary manner Discovery and Validation In-house and 3 rd party in vitro, in vivo, and human use study platforms Supporting Framework/Network Supported by NuLiv’s own subsidiaries and affiliates, and long term strategic partners and alliances in many distinguished academic and research institutions
  • 8.
  • 9. NuLiv Taipei Laboratory Has Access to Equipments to Perform a Wide Range of In Vitro and In Vivo Studies Laminar Flow, Cell Culture Water Purification System CO2 Incubator Lab Bench Spectrophotometer Photomicrographic Equipment High Speed Centrifuge PCR machine Gel Dryer Deep Fryer Gel Photosystem Animal Room National Defense Medical Center
  • 10.
  • 11. NuLiv Management /Business & Research Executives Founder & CEO Austin Wang, M.S. NuLiv Holdings, Inc. (World Headquarters) Taipei City, Taiwan Executive Director Y.H. Lee, M.D. Vice Chancellor, China Medical Univ. (Taiwan), Previously Dean, Chang Kung Medical School, Dep. Director, Chang Kung Hospital System (10,000+ beds) Principle Scientific Advisor H.C. Lin, Ph.D. Univ. of North Carolina. Taught TCM at NDMC for over 20 years. Pioneered the hypothesis that “Qi” herbs regulate absorption and other metabolic functions President Americas, Europe, Asia, Africa Business Development Sales & Marketing Representative Americas, Europe, Asia, Africa Michael Wang, M.S. NuLiv Science USA, Walnut, CA David Allen, Ph.D. World Wellness, LLC., San Diego, CA President Greater China David Wu, Ph.D. Business Administration, Univ. of South Australia, Director Research & Development NuLiv Taipei Laboratory Thomas Yang, Ph.D., Molecular Microbiology, Univ. of Texas, Southwestern Medical Center Director NuLiv Shanghai Laboratory Sunnie Sun, Ph.D., Pharm. Analysis, Sheng Yang Pharm. Univ. Director Clinical Research Rose Chen, M.D., Ph.D., Shanghai TCM University
  • 12. NuLiv’s Scientific Advisors Chang, T.C., Ph.D. Biochemistry, Univ. of Illinois, Professor, Dept. of Biochemistry, National Defense Medical Center,Taiwan Chang, W.L., Ph.D. Pharmacy, National Taiwan Univ., Professor, School. of Pharmacy, National Defense Medical Center, Taiwan Kuo, C.H., Ph.D. Physiokinetics, Univ. of Texas, Professor and Chair, Department of Exercise Science, National Taipei Physical Education College, Taiwan Chen, C.X., Professor Director, Department of Pharmacology, TCM Univ., Shanghai, China. Serves as our toxicity and safety advisor on TCM and other herbs Komatsu, Yasuhiro, Ph.D. Medical science, Juntendo University, Prior Dean of R&D department, Tsumura & Co. Chu, C. H, Ph.D. Food engineering, University of Tokyo, expert in pre-clinical pharmacology tests Fan, David, Ph.D. Senior Lecturer, Trinity College, Cambridge Univ., Editor-in-chief, Angiogenesis Hsieh, Steve, Ph.D. Nutritional Biochemistry, Univ. of Wisconsin, Ex Vice Chairman, National Science Council, R.O.C. Government Looi Hwang, Ph.D. Industrial & Environmental Pharmacy Ph.D. from Purdue, previously G.M. of MSD, Hanzhou, China & and Merck of Singapore J.K. Luo, Ph.D. Editor (acting), Journal of Heterocyclic Chemistry, Professor at University of S. Florida Funfack, Wolf, M.D. Metabolic Balance founder. Peng, C.K., Ph.D. Statistical Physics, Boston Univ., Assoc. Professor/Co-director, Rey Institute for Nonlinear Dynamics in Physiology and Medicine, Harvard Medical School.
  • 13.
  • 14. Our Supporting Framework & Network Facility, equipment, interdisciplinary consultation National Defense Medical Center Industrial Technology Research institute, Taiwan Yang Ming University, Taiwan Taipei Medical University, Taiwan In vivo and human study China Pharmaceutical University, Nanjing, China Taipei Physical Education College I-Shou University Hospital NuLiv Wellness Clinic Consultation, Cellular science Metabolic health TCM pharmacology & toxicology angiogenesis Yang Ming Univ. Stem Cell Research Center Taipei Medical Univ. Stem Cell Research Center Dr. Wolfgang Funfack, Metabolic Balance, De. Shanghai TCM university China Medical University, Taiwan Trinity college, Cambridge University, UK
  • 15. Patents from NuLiv’s Research Title Country/Region Patent NO. Application NO. Method for Regulating Nutrient Absorption with Astragalosides USA US11/426,029 China CN200610090113.0 Taiwan I271195 Method for Regulating Nutrient Absorption with Ginsenosides USA US11/426,06 China CN200610090114.5 CN200910178493.7 CN200910178492.2 Taiwan I317280 Method for Skin Care PCT PCT/US07/77326 USA US11/469,659 China CN200710089586.3 Taiwan TW095132614 Use of Cycloartane compounds for treating arthritis USA US12/353,779 China N/A yet Taiwan TW098135306
  • 16.
  • 17.
  • 18. 6 Fractions / Compounds from our Research and Their Biological Functions Fractions/Compounds Discovered Functions NL-F001 Anti-angiogeneis NL-F002 Enhance amino acids, vitamins, and glucose absorption at the cellular level; anti-angiogenesis NL-F003 Increase Adiponectin secretion Ergogenic/recovery NL-F004 Enhance amino acids, vitamins, and glucose absorption at the cellular level NL-F006 Enhance amino acids, vitamins, and glucose absorption at the cellular level ; Increase Adiponectin secretion NL-F007 Increase collagen and hyaluronic acid secretion; decrease MMP-1 secretion
  • 19.
  • 20. How ActiGin TM Works Creatine kinase MDA IL-6 Inflammatory biomarkers inhibition Exercise Performance Fast Recovery Exercise Oxidative stress elevation Glucose uptake Oxidative stress reduction ATP
  • 21.
  • 22. How Astrion TM Works
  • 24.
  • 25.
  • 26.
  • 27. Extraction, Fractionation, Separation, and Purification of Raw Herbs to Fraction and Compound Levels
  • 28. The Purity and Concentration Level of our Bioactive Fractions and Compounds are Identified and Measured by Specific Bioassays Active constituents Medicinal plants Crude extracts Fraction 1 Fraction 2 Fraction 3 Fraction 4 Fraction 5 Fraction 6 … fractionation extraction Formulation Depend on function Products & Marketing Structure elucidation UV MS NMR IR CD 1 H NMR 13 C NMR 2D NMR separation Absorption Anti-cancer Skin care, etc Active constituents QC systems -LC/UV -GC/MS Bioassay QC
  • 29. Our Supplements are Highly Effective and Safe in Supporting Critical Human Health Functions
  • 30. Our Sourcing Operations Guarantees Premier Quality Products with Competitive Prices and Timely Delivery to our Customers NuLiv/ Customer specifications/requirements Vendor Vendor Survey for Certificates, Qualifications, MOA, COA, sample Onsite Vendor Survey Disqualified Qualified Contract / Agreement Primary suppliers Secondary Supplier
  • 31.
  • 32. Futuristic Wellness Product and Service Platform (A NuLiv Experimental Model) Wellness Advisors Interactive and personal wellness information exchange and virtual community management NuLiv Wellness Management Inc. Device HRM Meridian Blood analysis CD/Biochips Nutritional Sciences NuLiv Laboratories (Slides 1 – 42) System complexity analysis DynaDx – Harvard Reylab Evidence based wellness know-how NuLiv Wellness Clinic Wellness/Sub-clinical Primary Care Clinic Cellular sciences Steminent Therapeutic Inc .
  • 33.