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Vibrio tubiashii  research update Tatyana Marushchak
Foot Experimet ,[object Object],Protein Gel:  distinct bands in the samples without foot;   smear in the samples with foot, but differnt size bands evident- possible proteases Gel was repeated – same results Isolation of the RNA:  There is significantly more RNA in the foot samples mass of the pellet: VT+foot-------0.86g   VT no foot---0.22g Spectrophotomer analysis: VT+foot-------1669.37 ng/ul   Vt no foot-----351.01 ng/ul Both samples were diluted to the concenration of app. 150 ng/ul PCR of the samples:  Primers used: 1. Vtubi_16s 2. Vtubi_chitinase 3. Vtubi_vthB VthB and Chitinase produced bands. Interesing absence of 16s bands
Gigas Hemocyte Challenge ,[object Object],[object Object],[object Object]
Exposure Experiment ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
PH experiment ,[object Object],[object Object],[object Object],[object Object]
Specificity of primers ,[object Object],[object Object],[object Object],[object Object]
Seed Experiment -1 st  slide ,[object Object],[object Object],[object Object],[object Object],[object Object]
Seed Experiment-2 nd  slide ,[object Object]
Temperature Experiment ,[object Object],[object Object],[object Object]

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