Thompson_MGpresentation

Development of Biomarkers for Stress in Octopus Rachel Thompson School of Aquatic and Fishery Sciences May 15, 2009

Background ,[object Object],[object Object],[object Object],[object Object],Giant Pacific Octopus ( E. dofleini ) Red Octopus ( O. rubescens )

Objectives ,[object Object],[object Object],[object Object],[object Object],[object Object]

Non-Invasive Techniques ,[object Object],[object Object],[object Object],[object Object]

Protein Gel Electrophoresis ,[object Object],[object Object],[object Object],[object Object],Container Underwater Skin

Protein Identification ,[object Object],[object Object]

Mass Spectroscopy ,[object Object],[object Object],[object Object],Description Sequence (P02662) Alpha-S1-casein precursor YLGYLEQ (P02662) Alpha-S1-casein precursor EPMIGVNQELAYFYPELFR (P02808) Statherin precursor RIGRFGYGYGPYQPVPEQPLYPQPYQPQYQQYTF (P81605) Dermicidin precursor [Contains: Survival-promoting peptide; DCD-1] DAVEDLESVGK

Proteins Isolated from Mucus ,[object Object],[object Object],[object Object],[object Object]

Heat Shock Proteins ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Western Blot - HSP 70 ,[object Object],[object Object],[object Object],[object Object]

Behavior Monitoring ,[object Object],[object Object],[object Object],Cephcam watch ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Behavior Observations ,[object Object],[object Object]

Objectives ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Egg Development Laid January, 2009 Refrain from imposing stressful conditions

Observation of Developmental Stages Early: 8 weeks

Late: 12 weeks - Appearance of chromatophores - Movement within egg

Egg Development ,[object Object],[object Object],[object Object],[object Object]

Developmental Genes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Real-Time PCR (qPCR) ,[object Object],[object Object],[object Object]

Orthodenticle-like protein (OTX) -Expressed early in development -100,000 fold increase over time

Hedgehog (Hh) -Expressed later in development <100 fold increase over time

Results-Gene Expression ,[object Object],[object Object],[object Object],[object Object],[object Object]

Behavior Observations Prior to Egg-Laying Post Egg-Laying

Summary ,[object Object],[object Object],[object Object],[object Object]

Applications and Future Work ,[object Object],[object Object],[object Object]

Acknowledgements ,[object Object],[object Object],[object Object],[object Object]

Thompson_MGpresentation

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