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The document summarizes research characterizing the toll-interacting protein (TOLLIP) gene in the black abalone (Haliotis cracherodii). The researcher obtained partial TOLLIP sequence information for black abalone and identified other toll-like receptor genes. Quantitative PCR analysis found no significant difference in TOLLIP expression levels between control and bacterium-exposed black abalone digestive gland and gill tissues. Future work will further explore the toll-like receptor pathway and compare laboratory results to field data.

Technologie
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Characterization of a toll-interacting protein (TOLLIP) gene in the black abalone ( Haliotis cracherodii ) Cullen Taplin May 16, 2008
Presentation Outline ,[object Object],[object Object],[object Object],[object Object],[object Object]
Black Abalone ,[object Object],[object Object],[object Object]
Abalone Distribution in Western U.S. ,[object Object]
Black Abalone Declines ,[object Object],[object Object],[object Object],[object Object],[object Object],1986 1988 1999
Withering Syndrome ,[object Object],[object Object],Healthy black abalone (left) and one suffering from withering syndrome (right). J. Moore
Abalones’ Immune Response ,[object Object],[object Object],[object Object]
Identifying Immune Related Genes ,[object Object],[object Object],[object Object]
Toll-like Receptor Pathway ,[object Object],[object Object],Singh, Bhanu P., R. S. Chauhan, and Kokesh K. Singhal. 2003. Toll-like Receptors And Their Role In Innate Immunity . Current Science. vol. 85, no. 8, pgs. 1156 – 1164.
Research Goal & Objectives ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Abalone Used In Research ,[object Object],[object Object]
Research Methods ,[object Object],[object Object],[object Object],[object Object],[object Object]
Partial TOLLIP sequence and comparison to the Pacific abalone ( H. discus ) ,[object Object],Results: Sequence Information
TOLLIP Sequencing Results (cont.) ,[object Object],[object Object],[object Object],10kbp – 4kbp – 2kbp – 800bp –
Results: Discovering Other TLR Genes ,[object Object],[object Object],[object Object],[object Object],Protein Analysis Through Evolutionary Relationships www.pantherdb.org/
Results: TOLLIP Expression in Digestive Gland Tissue Five months after initial exposure. N=8 for control groups, N=9 exposed groups. Means were not significantly different (p > 0.05).
Results: TOLLIP Expression in Gill Tissue Eight months after initial exposure. N = 6 for both treatment groups. Means were not significantly different (p > 0.05).
Results: Correlation of TOLLIP Expression in and Bacterial Loads No significant correlations between TOLLIP expression and bacterial loads (p > 0.05).
Summary of Results ,[object Object],[object Object],[object Object],[object Object],Data suggests the TLR pathway is active in gill tissue.
Future Work ,[object Object],[object Object],[object Object]
Acknowledgements ,[object Object],[object Object],[object Object],[object Object],[object Object]
Questions?

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Taplin Capstone

  • 1. Characterization of a toll-interacting protein (TOLLIP) gene in the black abalone ( Haliotis cracherodii ) Cullen Taplin May 16, 2008
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  • 16. Results: TOLLIP Expression in Digestive Gland Tissue Five months after initial exposure. N=8 for control groups, N=9 exposed groups. Means were not significantly different (p > 0.05).
  • 17. Results: TOLLIP Expression in Gill Tissue Eight months after initial exposure. N = 6 for both treatment groups. Means were not significantly different (p > 0.05).
  • 18. Results: Correlation of TOLLIP Expression in and Bacterial Loads No significant correlations between TOLLIP expression and bacterial loads (p > 0.05).
  • 19.
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  • 21.