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CHARACTERIZING DISEASE RESISTANCE IN NATIVE OYSTERS  That Have Experienced Disease Pressure Roxanna Smolowitz, Steven Roberts, Jackie DeFaveri Christina Romano, Rick Karney
Oyster Disease ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Research Objectives ,[object Object],[object Object],[object Object],[object Object]
Oysters that had experienced disease pressure Oysters that had not experienced disease pressure (naïve) Spawn Spawn Experimental Design
Oysters (~4000 from each group) were grown in a Dermo prone site- Edgartown Great Pond July 2005 - Present
overwintered on bottom Temperature Data
Mortality Naïve oysters Survivors of  Disease  Pressure
Survivors of Dermo pressure [Edgartown GP] Naive [Tisbury]
DERMO Perkinsus marinus Survivors of Dermo pressure [Edgartown GP] Naive [Tisbury]
Research Objectives ,[object Object],[object Object],[object Object],[object Object]
Photo: Gary H. Wikfors: Northeast Fisheries Science Center,  NOAA Fisheries Milford Laboratory, Milford, CT   Immune Response in Oysters
What are the biochemical or  gene products  produced by hemocytes?
Approach: Genetic Characterization ,[object Object],[object Object],[object Object]
CIAPIN Expression cytokine induced apoptosis inhibitor 1 Survivors of Dermo pressure [Edgartown GP] Naive [Tisbury] Apoptosis (programmed cell death) regulation  is likely involved in disease resistance
Survivors of Dermo pressure [Edgartown GP] Naive [Tisbury] Cathepsin L Expression Cathepsins are a family of proteases  Proteases and protease inhibitors have been shown to play critical roles in immunity and host-parasite interactions.  One possibility is Cathepsin L is involved in  P. marinus  defense activity.
For more information….
Conclusions ,[object Object],[object Object]

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Disease Research - Oysters

  • 1. CHARACTERIZING DISEASE RESISTANCE IN NATIVE OYSTERS That Have Experienced Disease Pressure Roxanna Smolowitz, Steven Roberts, Jackie DeFaveri Christina Romano, Rick Karney
  • 2.
  • 3.
  • 4. Oysters that had experienced disease pressure Oysters that had not experienced disease pressure (naïve) Spawn Spawn Experimental Design
  • 5. Oysters (~4000 from each group) were grown in a Dermo prone site- Edgartown Great Pond July 2005 - Present
  • 6. overwintered on bottom Temperature Data
  • 7. Mortality Naïve oysters Survivors of Disease Pressure
  • 8. Survivors of Dermo pressure [Edgartown GP] Naive [Tisbury]
  • 9. DERMO Perkinsus marinus Survivors of Dermo pressure [Edgartown GP] Naive [Tisbury]
  • 10.
  • 11. Photo: Gary H. Wikfors: Northeast Fisheries Science Center, NOAA Fisheries Milford Laboratory, Milford, CT Immune Response in Oysters
  • 12. What are the biochemical or gene products produced by hemocytes?
  • 13.
  • 14. CIAPIN Expression cytokine induced apoptosis inhibitor 1 Survivors of Dermo pressure [Edgartown GP] Naive [Tisbury] Apoptosis (programmed cell death) regulation is likely involved in disease resistance
  • 15. Survivors of Dermo pressure [Edgartown GP] Naive [Tisbury] Cathepsin L Expression Cathepsins are a family of proteases Proteases and protease inhibitors have been shown to play critical roles in immunity and host-parasite interactions. One possibility is Cathepsin L is involved in P. marinus defense activity.
  • 17.