1. Aminoacyl tRNA Synthetase
Somanna A. N.

2. Fig: 2 steps of aminoacyl tRNA charging. (a) Adenylylation of amino acid
(b) Transfer of adenylylated amino acid to tRNA

3. tRNA charging

4. Classes of Aminoacyl tRNA Synthetases

5. Structure of tRNA: Elements required for aminoacyl
synthetase recognition

6. Co-crystal structure of glutaminyl aminoacyl tRNA synthetase
with tRNA-Glu
Enzyme is shown in grey and tRNA in purple. The yellow, red and green is Glutaminyl
AMP

7. Accuracy of Aminoacyl tRNA formation
Distinguishing features of similar amino acid

8. Proofreading

9. Editing of Aminoacyl-tRNA.
The flexible arm of an aminoacyl-tRNA can move the amino acid between the activation
site and the editing site. If the amino acid fits well into the editing site, the amino acid is
removed by hydrolysis.

10. Cysteinyl tRNA charged with Cysteine or Alanine