detail on solid lipid nano particles (sln's)

1. SOLID LIPID NANOPARTICLES
PREPARED BY:- SHREESHAIL TUMBAGI
1ST M.PHARMACY
PHARMACETICS
ABMRCP-107

2. CONTENTS: -
INTRODUCTION
DEFINITION
METHOD OF PREPARATIONS
EVALUATION
ADVANTAGES
DISADVANTAGES
APPLICATION
REFERENCES
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3. INTRODUCTION :-
Solid lipid nanoparticles (SLN) are a new pharmaceutical delivery
system or pharmaceutical formulation
Solid lipid nanoparticles possess a solid lipid core matrix that can
solubilize lipophilic molecules. The lipid core is stabilized
by surfactants (emulsifiers)
Solid lipid nanoparticles are spherical in shape & diameter range from 10-1000nm.
They are manufactured from synthetic/natural polymers and ideally suited to
optimize drug delivery and reduce toxicity
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4. Structure of solid lipid nanoparticle
(SLN)
DEFINITION :-
• The Solid Lipid Nanoparticles (SLN’S) Are Submicron Colloidal Carriers Which
Are Composed Of Physiological Lipid, Dispersed In Water Or In An Aqueous
Surfactant Solution
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6. METHOD OF PREPARATIONS :-
SLNs are made up of solid lipid, emulsifier and water/solvent. The lipids used may be triglycerides (tri-
stearin), partial glycerides, fatty acids (stearic acid, palmitic acid), and steroids (cholesterol) and waxes
(cetyl palmitate).
Different methods of SLNs preparation :-
o High shear homogenization:
 Hot homogenization
 Cold homogenization
o Ultra sonication / high speed homogenization:
 Probe ultra sonication
 Bath ultra sonication
o Solvent emulsification/evaporation
o Micro emulsion based SLN preparations
o SLN preparation by using supercritical fluid
o Spray drying method
o Double emulsion method
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7. HIGH SHEAR HOMOGENIZATION:-
It is A reliable and powerful technique, which is used for the production of slns.
High pressure homogenizers push A liquid with high pressure (100–2000 bar)
through A narrow gap (in the range of A few microns). The fluid accelerates on A
very short distance to very high velocity (over 1000 km/H). Very high shear stress
and cavitation forces disrupt the particles down to the submicron range. Generally
5 -10% lipid content is used but up to 40% lipid content has also been investigated.
Two general approaches of HPH are :-
 Hot Homogenization.
 Cold Homogenization.
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8. HOT HOMOGENIZATION :- COLD HOMOGENIZATION :-
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9. ULTRA SONICATION OR HIGH SPEED HOMOGENIZATION :-
SLN were also developed by high speed stirring or sonication.
The problem of this method is broader particle size distribution ranging into
micrometre range.
This lead physical instabilities likes particle growth upon storage. Potential
metal contamination due to ultra sonication is also a big problem in this
method.
So for making a stable formulation, studies have been performed by various
research groups that high speed stirring and ultra sonication are used combined
and performed at high temperature.
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10. SPRAY DRYING METHOD :-
It’s an alternative procedure to lyophilisation in order to transform an aqueous
SLN dispersion into a drug product.
It’s a cheaper method than lyophilisation.
This method cause particle aggregation due to high temperature, shear forces
and partial melting of the particle
SLN PREPARED BY SOLVENT EMULSIFICATION/EVAPORATION :-
For the production of nanoparticle dispersions by precipitation in o/w
emulsions. The lipophilic material is dissolved in water-immiscible organic
solvent (cyclohexane) that is emulsified in an aqueous phase.
Upon evaporation of the solvent nanoparticle dispersion is formed by
precipitation of the lipid in the aqueous medium.
 The mean diameter of the obtained particles was 25 nm with cholesterol acetate
as model drug and lecithin/sodium glycocholate blend as emulsifier
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11. INGREDIENTS USED IN THE PREPARATION OF SLN :-
Name of the ingredients concentration
Lipid 3.33% w/v
Phospholipids 0.6-1.5%
Glycerol 2-4%
Poloxamer 188 1.2-5% w/w
Soy phosphatidyl choline 95%
Compritol 10%
Cetyl palmitate 10% w/w
Tego care 450 (surfactant) 1.2% w/w
PEG 2000 0.25%
PEG 4500 0.5%
Tween 85 0.5%
Ethyl oleate 30%
Na alginate 70%
Ethanol/butanol 2%
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12. EVALUATION :-
EX VIVO METHODS :-
A large number of drugs including very hydrophilic molecules have been
postulated to be
incorporated into SLN.
Various methods used to study the in vitro release of the drug are:
• side by side diffusion cells with artificial or biological membrane.
• Dialysis bag diffusion technique.
• reverse dialysis bag technique.
• Agitation followed by ultracentrifugation or centrifugal ultra filtration. 12

13. IN VITRO DRUG RELEASE :-
DIALYSIS TUBING:-
In vitro drug release could be achieved using dialysis tubing. The solid lipid nanoparticle
dispersion is placed in pre - washed dialysis tubing which can be hermetically sealed. The
dialysis sac then dialyzed against a suitable dissolution medium at room temperature; the
samples are withdrawn from the dissolution medium at suitable intervals, centrifuged and
analyzed for the drug content using a suitable analytical method.
REVERSE DIALYSIS:-
In this technique a number of small dialysis sacs containing 1 ml of dissolution medium are
placed in SLN dispersion. The SLN’s are then displaced into the medium.
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14. ADVANTAGES
Control And/or Target Drug Release.
Improve Stability Of Pharmaceuticals.
High And Enhanced Drug Content (Compared To Other Carriers).
Feasibilities Of Carrying Both Lipophilic And Hydrophilic Drugs.
Most Lipids Being Biodegradable, SLN’s Have Excellent Biocompatibility.
Water Based Technology (Avoid Organic Solvents).
Easy To Scale-up And Sterilize.
More Affordable (Less Expensive Than Polymeric/Surfactant Based Carriers).
Easier To Validate And Gain Regulatory Approval.
Much Easier To Manufacture Than Bio Polymeric Nanoparticles.
No Special Solvent Required.
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15. DISADVANTAGES
› Particle growth.
› Unpredictable gelation tendency.
› Unexpected dynamics of polymeric transitions.
› Sometimes burst release.
APPLICATION
Used as Target drug delivery system.
 Used in Topical use, cosmeceuticals.
 Targeted carrier for anticancer drug to solid tumours. 15

16. REFERENCES :-
• SAUPE, ANNE; RADES, THOMAS (2006). "NANOCARRIER TECHNOLOGIES".
P. 41. DOI:10.1007/978-1-4020-5041-1_3.
• JENNING, V; THUNEMANN, AF; GOHLA, SH (2000). "CHARACTERISATION OF A
NOVEL SOLID LIPID NANOPARTICLE CARRIER SYSTEM BASED ON BINARY
MIXTURES OF LIQUID AND SOLID LIPIDS". INTERNATIONAL JOURNAL OF
PHARMACEUTICS 199 (2): 167–77
• S. MUKHERJEE*, S. RAY AND R. S. THAKUR, SOLID LIPID NANOPARTICLES: A
MODERN FORMULATION APPROACH IN DRUG DELIVERY SYSTEM.
• HTTP://EN.WIKIPEDIA.ORG/WIKI/SOLID_LIPID_NANOPARTICLE 16

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