8. NPs for Water Remediation Nanoparticles Contaminant Reference Nano Zeolites Toluene, nitrogen dioxide Song et al. (2004) Carbon nanotubes (CNTs) p-nitrophenol, benzene, toluene, heavy metal ions,dimethylbenzene Jin et al. (2007) Bimetallic nanoparticles (Pd/Fe) PCBs, chlorinated methane, ethene, Trichloroethylene (TCE) Xu and Bhattacharyya (2005) Xu and Zhao (2007) Wang and Zhang (1997) Kim et al. (2010) Ni/Fe and Pd/Au nanoparticles TCE and PCBs He and Zhao (2005) TiO 2 photocatalyst Heavy metal ions Pentachlorophenol (PCP) Pena et al. (2005) Quan et al. (2005) NZVI Herbicides (Alachlor) Thompson et al. (2010) NZVI Nitrate Bezbaruah et al. (2009) NZVI TCE Bezbaruah et al. (2011)
24. Experimental Design Microorganisms Pp F1 D. BAV1 Encapsulated Microorganisms Combined NZVI-Microorganism System TCE Degradation NZVI Bare Encapsulated Encapsulated NZVI Research Phase I
30. Preparation of Capsules a : Capsules 30 mg NZVI 1.33% Iron loss Bezbaruah et al., 2011 b b : Encapsulated NZVI 4 g Maltodextrin 6 mL DI water 0.25 g CaCl 2 50 mL alginate Peristaltic pump Stirrer 6 cm b a
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35. TCE Degradation Kinetics TCE degradation followed First Order Kinetics for both bare and encapsulated NZVI No significant difference Bare and Encapsulated NZVI (95% CI, Two Way ANOVA ) Bezbaruah et al., 2011 Batch Initial TCE concentration mg L -1 Reaction rate constant R 2 k obs 10 -2 min -1 k sa 10 -3 L m -2 min -1 Bare NZVI 1 2.92 1.6 0.9689 10 2.35 1.3 0.9801 30 1.53 0.8 0.9897 40 2.24 1.2 0.9868 Encapsulated NZVI 1 3.23 1.7 0.9832 10 2.45 1.3 0.9491 30 1.92 1.0 0.9921 40 2.21 1.2 0.9425
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39. Work Remaining Microorganisms Pp F1 D. BAV1 Encapsulated Microorganisms Combined NZVI-Microorganism system TCE Degradation NZVI Bare Encapsulated Encapsulated NZVI
40. Experimental Design Microorganisms Pp F1 D. BAV1 Encapsulated Microorganisms Combined NZVI-Microorganism system TCE Degradation NZVI Bare Encapsulated Encapsulated NZVI Research Phase II
42. TCE Degrading Bacteria Microorganism Method of Removal End Product Reference Pseudomonas putida F1 Direct Oxidation Co-metabolism ETH (Sun and Wood 1996); (Kim et al. 2010b); (Radway et al. 1998) Clostridium bifermentans DPH-120 Co-metabolism c-DCE (Chang et al. 2000) Dehalospirillum multivorans Direct Oxidation c-DCE (Neuman et al.1994) Dehalococcoides ethenogenes 195 Reductive dechlorination ETH (Maymo-Gatell et al. 1999), (Fennell et al. 2001) Dehalococcoides BAV1 Reductive dechlorination ETH (Krajmalnik-Brown et al. 2004; Maymo-Gatell et al. 1999),(He et al. 2003) Dehalococcoides VS Reductive dechlorination ETH (Cupples et al. 2003) Dehalococcoides FL2 Reductive dechlorination ETH (El Fantroussi et al. 1998)
47. Growth Curve- Pseudomonas putida F1 P. putida incubated at 30 o C in TSB media and shaken for 24 h 1 mL aliquot (0-24 h ) Serial dilution and nutrient agar plating Incubated for 24 h at 30 o C Plate count and plotting of Viable Cells/mL vs Time
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59. Work Remaining TCE Degradation NZVI Bare Encapsulated Encapsulated NZVI Microorganisms Pp F1 D. BAV1 Encapsulated Microorganisms Combined NZVI-microorganism system Research Phase III
63. D .BAV1 growth Studies D. BAV1 incubated at 22±2 o C in (MSM) media and shaken 1 mL aliquot at regular interval (0-36 h ) Serial dilution and nutrient agar plating Incubated for 24 h at 22±2 o C Plate count and plotting of Viable Cells/mL vs Time
Zeolites are aluminosilicate materials commonly used as adsorbents
Iron core shell --- oxidized--- electron donor (so lises e-) The organic compound is reduced
aquifer is a wet underground layer of water-bearing permeable rock
Each step Cl- is knocked out Benign product ---ethene
1.Borohydride is dropped into ferrous 2.Vaccum dried(figure a and b) 3. Agglomeration 4.XRD peak indicative of Fe0 not oxide!
2 factor: factor 1(2 treatments- – bare and encapsulated) Factor 2: (concentration) 4 concentrations –triplicates ---2 factors(bare and encapsulated) 24 experimental k values More interested in type of NZVI Difference in treatments means (k) difference in treatment means concentration!(same type of reaction) Ho- there is no difference in means of K Ha: there is a difference in means for Conc: p=o reject Ho for bare/encapsulated : p=0.211 accept ho alpha =0.05
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