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Photosynthesis
What is photosynthesis?
• Photo = light
• Synthesis = the making of something by combining
separate components
• Photosynthesis is the process of converting light energy
into chemical energy that is store in carbohydrates and
other organic molecules
• A process that is carried out by photosynthetic autotrophs
such as plants, protists (i.e. algae), and some bacteria
• Occurs in the chloroplasts
The location and structure of chloroplasts
LEAF CROSS SECTION MESOPHYLL CELL
LEAF
Chloroplast
Mesophyll
CHLOROPLAST Intermembrane space
Outer
membrane
Inner
membrane
Thylakoid
compartmentThylakoidStroma
Granum
StromaGrana
What are the reactants & Products?
The goal is to know when and how each reactant is used during the process of
photosynthesis
What are the two stages of photosynthesis?
• The Calvin cycle makes
sugar from carbon
dioxide
– ATP generated by the light
reactions provides the energy
for sugar synthesis
– The NADPH produced by the
light reactions provides the
electrons for the reduction of
carbon dioxide to glucose
Light
Chloroplast
Light
reactions
Calvin
cycle
NADP+
ADP
+ P
The light reactions
convert solar energy
to chemical energy
• Produce ATP & NADPH
AN OVERVIEW OF PHOTOSYNTHESIS
Structure & Function of
a photosystem
Components of a photosystem:
•Light harvesting complex = pigment
molecules attached to proteins
•Reaction Center Complex = proteins
that contain a special pair of
chlorophyll a molecules
What happens in a photosystem:
1.Chlorophyll pigment absorbs
light
2.Energy transferred from
pigment to pigment
3.Energy reaches reaction center
where special chlorophyll a uses
the energy to launch an e- to a
higher energy level
4. primary e- acceptor captures
e-
Study this figure of How a photosystem harvests light
Thylakoidmembrane
Photon
Photosystem STROMA
Light-
harvesting
complexes
Reaction-
center
complex
Primary
electron
acceptor
Transfer
of energy
Special pair of
chlorophyll a
molecules
Pigment
molecules
THYLAKOID SPACE
(INTERIOR OF THYLAKOID)
e−
Photon
Photon
Water-splitting
photosystem
NADPH-producing
photosystem
ATP
mill
Two types of photosystems
cooperate in the light reactions
Cytochrome
complex
Primary
acceptor
Primary
acceptor
H2O
O2
2 H+
+
1
/2
P680
Light
Pigment
molecules
Photosystem II
(PS II)
Photosystem I
(PS I)
Pq
Pc
ATP
1
2
3
5
6
7
8
Electron transport chain
Electron
transport
chain
P700
Light
+ H+
NADP+
NADPH
NADP+
reductase
Fd
e−
e−
e−
e−
4
e−
e−
• Water is split, generating molecular oxygen, stripping electrons
for electron transport chain and leaving H+ to remain
• The flow of electrons through photosystems during light
reaction lead to the production of NADPH and ATP
Photosynthesis:
Light Reactions and Chemiosmosis
STROMA
(low H+
concentration)
STROMA
(low H+
concentration)
THYLAKOID SPACE
(high H+
concentration)
Light
Photosystem II
Cytochrome
complex Photosystem I
Light
NADP+
reductase
NADP+
+ H+
To
Calvin
Cycle
ATP
synthase
Thylakoid
membrane
2
1
3
NADPH
Fd
Pc
Pq
4 H+
4 H+
+2 H+
H+
ADP
+
P i
ATP
1
/2
H2O
O2
H+
H+
H+
H+
2 H+
+ 1
/2
Water-splitting
photosystem
Reaction-
center
chlorophyll
Light
Primary
electron
acceptor
Energy
to make
Electron transport chain
Primary
electron
acceptor
Primary
electron
acceptor
NADPH-producing
photosystem
Light
NADP+
1
2
3
Summary: How the Light Reactions Generate ATP and NADPH
BIG PICTURE:
•Aka Dark Reactions or Light-
independent reactions
•Anabolic process: making
carbohydrates from smaller
molecules
•Carbon in the form of CO2 goes
into the cycle, and comes out in the
form of sugar.
•ATP and NADPH from the light
reactions serve as an energy
sources that drives the conversion
of CO2 to glucose
•3-carbon sugar is produced with
each turn of the cycle
Calvin Cycle
Question: Will the Calvin Cycle occur in
the dark? WHY or WHY NOT?
Review: Photosynthesis uses light energy to make
food molecules
Light
Chloroplast
Photosystem II
Electron
transport
chains
Photosystem I
CALVIN
CYCLE Stroma
Electrons
LIGHT REACTIONS CALVIN CYCLE
Cellular
respiration
Cellulose
Starch
Other
organic
compounds
Connections: What is the
relationship between the light
reactions and the Calvin
Cycle?
Create your own mindmap of
photosynthesis

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Photosynthesis

  • 2. What is photosynthesis? • Photo = light • Synthesis = the making of something by combining separate components • Photosynthesis is the process of converting light energy into chemical energy that is store in carbohydrates and other organic molecules • A process that is carried out by photosynthetic autotrophs such as plants, protists (i.e. algae), and some bacteria • Occurs in the chloroplasts
  • 3. The location and structure of chloroplasts LEAF CROSS SECTION MESOPHYLL CELL LEAF Chloroplast Mesophyll CHLOROPLAST Intermembrane space Outer membrane Inner membrane Thylakoid compartmentThylakoidStroma Granum StromaGrana
  • 4. What are the reactants & Products? The goal is to know when and how each reactant is used during the process of photosynthesis
  • 5. What are the two stages of photosynthesis?
  • 6. • The Calvin cycle makes sugar from carbon dioxide – ATP generated by the light reactions provides the energy for sugar synthesis – The NADPH produced by the light reactions provides the electrons for the reduction of carbon dioxide to glucose Light Chloroplast Light reactions Calvin cycle NADP+ ADP + P The light reactions convert solar energy to chemical energy • Produce ATP & NADPH AN OVERVIEW OF PHOTOSYNTHESIS
  • 7. Structure & Function of a photosystem Components of a photosystem: •Light harvesting complex = pigment molecules attached to proteins •Reaction Center Complex = proteins that contain a special pair of chlorophyll a molecules What happens in a photosystem: 1.Chlorophyll pigment absorbs light 2.Energy transferred from pigment to pigment 3.Energy reaches reaction center where special chlorophyll a uses the energy to launch an e- to a higher energy level 4. primary e- acceptor captures e- Study this figure of How a photosystem harvests light Thylakoidmembrane Photon Photosystem STROMA Light- harvesting complexes Reaction- center complex Primary electron acceptor Transfer of energy Special pair of chlorophyll a molecules Pigment molecules THYLAKOID SPACE (INTERIOR OF THYLAKOID) e−
  • 9. Cytochrome complex Primary acceptor Primary acceptor H2O O2 2 H+ + 1 /2 P680 Light Pigment molecules Photosystem II (PS II) Photosystem I (PS I) Pq Pc ATP 1 2 3 5 6 7 8 Electron transport chain Electron transport chain P700 Light + H+ NADP+ NADPH NADP+ reductase Fd e− e− e− e− 4 e− e− • Water is split, generating molecular oxygen, stripping electrons for electron transport chain and leaving H+ to remain • The flow of electrons through photosystems during light reaction lead to the production of NADPH and ATP
  • 10. Photosynthesis: Light Reactions and Chemiosmosis STROMA (low H+ concentration) STROMA (low H+ concentration) THYLAKOID SPACE (high H+ concentration) Light Photosystem II Cytochrome complex Photosystem I Light NADP+ reductase NADP+ + H+ To Calvin Cycle ATP synthase Thylakoid membrane 2 1 3 NADPH Fd Pc Pq 4 H+ 4 H+ +2 H+ H+ ADP + P i ATP 1 /2 H2O O2 H+ H+ H+ H+
  • 11. 2 H+ + 1 /2 Water-splitting photosystem Reaction- center chlorophyll Light Primary electron acceptor Energy to make Electron transport chain Primary electron acceptor Primary electron acceptor NADPH-producing photosystem Light NADP+ 1 2 3 Summary: How the Light Reactions Generate ATP and NADPH
  • 12. BIG PICTURE: •Aka Dark Reactions or Light- independent reactions •Anabolic process: making carbohydrates from smaller molecules •Carbon in the form of CO2 goes into the cycle, and comes out in the form of sugar. •ATP and NADPH from the light reactions serve as an energy sources that drives the conversion of CO2 to glucose •3-carbon sugar is produced with each turn of the cycle Calvin Cycle Question: Will the Calvin Cycle occur in the dark? WHY or WHY NOT?
  • 13. Review: Photosynthesis uses light energy to make food molecules Light Chloroplast Photosystem II Electron transport chains Photosystem I CALVIN CYCLE Stroma Electrons LIGHT REACTIONS CALVIN CYCLE Cellular respiration Cellulose Starch Other organic compounds Connections: What is the relationship between the light reactions and the Calvin Cycle?
  • 14. Create your own mindmap of photosynthesis