2. Silica gel or non-porous, meso porous or macro porous polymer
beads may be used as the packaging material in the column.
The greatest advantage of these columns is that these can be
cleaned with solvents and reused. The whole process is easy
and less laborious than the traditional chromatography
techniques. The HPLC has revolutionized the research industry
with better and faster results in biomolecule separation and
purification.
3. Every step in protein analysis from
separation, identification,
purification to quantification can be
done easily with the help of HPLC.
The column is packed with a
stationery phase that binds the
protein molecules on its surface. The
mobile phase or solvent is then used
to separate the desired protein on the
basis of its charge or nature from the
mixture and collected separately.
4. Use of HPLC columns for protein purification ?
HPLC columns are also used for
protein purification from a
separated sample. Purification is
based on a specific property of
the target protein. Depending
on the type of chromatography
used, the protein purified with
HPLC columns may be
functional or non-functional.
5. Get Me Touch
BioChrom Labs, Inc.
1719 South 13th Street
P.O. Box 2404
Terre Haute, Indiana 47802 U.S.A.
Telephone: (812) 234-2558;
Toll Free: (800) 545-2148
Fax: (812) 234-2461
Email: info@biochrom.com
Website: www.biochrom.com