Strengths and Weaknesses

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Strengths and Weaknesses Lisa Joseph Sophie Harris

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Weaknesses ,[object Object],[object Object],[object Object]

Points for Discussion ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Result reliability/reproducibility? Itoh Y et al CHO-hGPR40 <300µM Briscoe CP et al HEK293-hGPR40 >1µM Kotarsky K et al HFF11-R10 ‘ appropriate’ Capric acid (C6) 43±2.2 4.85±0.06 12.6±0.4 γ -linolenic acid 4.6±1.6 5.05±0.12 28.5±2.6 Oleic acid 2.0±0.3 4.39 123.1±12

Physiological conditions? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Intracellular metabolism or GPR40? Poitout 2003

Is it valuable? ,[object Object],[object Object],[object Object],[object Object]

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Strengths and Weaknesses

1. Strengths and Weaknesses Lisa Joseph Sophie Harris

6. Result reliability/reproducibility? Itoh Y et al CHO-hGPR40 <300µM Briscoe CP et al HEK293-hGPR40 >1µM Kotarsky K et al HFF11-R10 ‘ appropriate’ Capric acid (C6) 43±2.2 4.85±0.06 12.6±0.4 γ -linolenic acid 4.6±1.6 5.05±0.12 28.5±2.6 Oleic acid 2.0±0.3 4.39 123.1±12

8. Intracellular metabolism or GPR40? Poitout 2003