09th March 2011

1. Development of an ex-vivo three-dimensional model of Morphology of porous structure using scanning electron microscopy (SEM)
chronic lymphocytic leukaemia (CLL) by using biopolymers (PHAs) PHB 4% (w/v) PHB 4% (w/v) - Enlarged
Novelty: Be able to fabricate porous 3-D scaffolds with an improved thickness of
> 4 mm from PHB and PHBV and to model the ex-vivo 3-D abnormal BM niches
Solvent evaporation
Polymer solution Porogen + DIW
(Complied with UK-SED,
in organic solvent Polymer leaching
2002: <20 mg/m3)
solution +
Porogen PHBV 4% (w/v) PHBV 4% (w/v) - Enlarged
2 3 4 Porous 3-D 5
scaffolds
1
+ Polymer +
Polymer +
Solvent + Porogen cast
Porogen cast
Porogen (i.e., NaCl, Rectangular size of
sucrose etc.) polymeric porous 3-D
6
scaffolds (> 4mm) Physical properties of polymeric porous 3-D scaffolds
Solvent-Casting Particulate Leaching
Morphology of porous 3-D scaffolds of 4% (w/v)
PHB 4% (w/v) PHBV 4% (w/v)
morphology of porous 3-D scaffolds of 4% (w/v)
Macro-indentation test of
polymeric porous 3-D scaffolds
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SIDE
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SIDE
PHBV 4% (w/v) Both polymers have a good mechanical strength and
highly flexible materials as compared with porous
PHB 4% (w/v) ∼10 mm biodegradable composites & porous biodegradable
∼10 mm
polymers
∼5 mm PU 3-D foams - Laleh et al. Langmuir, 2006
INNER
INNER
SIDE
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2. Physical properties pre-
Surface treatment of PHB and PHBV porous 3-D and post surface treatment
scaffolds with an improved thickness to enhance Surface roughness was
found to be significantly
cell biomaterial adhesion and interactions affected by the treatment
except porosity which
remained quite similar
Water contact angle post treatment with more voids were
developed where
otherwise covered by the
thin films on the main
pores
In vitro degradation study of polymeric porous 3-D scaffolds in growth media
Morphology of porous structure using scanning electron microscopy (SEM)
0.4 M NaOH PHB 0.4 M NaOH PHBV
0.6 M NaOH PHB 0.6 M NaOH PHBV Effect of surface treatments on cell-biomaterial affinity of polymeric porous 3-D scaffold
Type of cell: Human chronic lymphocytic leukemia cell line (RL)
All untreated and
treated polymeric foams
were comparatively
PHB rf-oxygen plasma PHBV rf-oxygen plasma similar with all of them
exhibited high cell
number after 14 days