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“ Development of an  ex vivo  3-D model of chronic lymphocytic leukemia (CLL)” SAIFUL IRWAN ZUBAIRI SUPERVISOR:  Dr. Sakis Mantalaris  CO-SUPERVISOR: Dr. Nicki Panoskaltsis
Brief research introduction:   ,[object Object],[object Object],[object Object]
Continue:   ,[object Object],[object Object],[object Object],[object Object]
Aim and hypothesis 1  (MPhil level): ,[object Object],[object Object]
Aim and hypothesis 2  (MPhil level):   ,[object Object],[object Object],[object Object]
Aim and hypothesis 3  (MPhil level): ,[object Object],[object Object],[object Object],[object Object]
What is leukemia in relation with BM?   ,[object Object],[object Object],[object Object],[object Object]
Continue: ,[object Object],[object Object],[object Object],[object Object],[object Object]
Combining these two classifications provides a total of four main categories: ,[object Object],[object Object],[object Object]
What is chronic lymphocytic leukemia (CLL)? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
What is Polyhydroxylbutyrate (PHB)? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Ralstonia eutrophus  a.k.a  Alcaligenes eutrophus ,[object Object],[object Object],[object Object],[object Object]
Metabolic Pathway for PHB accumulation   ,[object Object],[object Object],[object Object],[object Object],[object Object],R-chain side PHB - consist - combination of 20,000 monomers
Why PHB but not other classes of polyester (e.g.: PHA, PHV, PHH & etc.)?   ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Research Approach (MPhil Level)
(1) Acquiring the polyhydroxybutryrate (PHB) - Bacterial batch and fed-batch fermentation process: ,[object Object],[object Object],[object Object],[object Object]
Continue: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
(2) Production of scaffolds: Fabrication and characterization ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Type of polymeric materials which are used for the fabrication of scaffolds and comparison purposes:
(3) Product testing (scaffolds + cells): Evaluating the cell proliferation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Cell seeding and proliferating observation of 4 different types of human’s CLL cell lines into   3 different types of polymeric materials
“ THANK YOU FOR YOUR KIND ATTENTION”
Metabolic Pathway: Continue

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Polymeric Porous 3-D Scaffolds

Hinweis der Redaktion

  1. (Pfeffer, 1992; Salehizadeh and Van Loosdrecht, 2004) – References quoted from Koutinas et al., 2006 (Anshuman et al., 2006) - PHB FROM FOOD WASTE AS A CARBON SOURCES FILES – NO. 3 – END NOTE
  2. I’ve identified 3 main objectives in my MPhil research project. I’ve found a few journals that used renewable feedstocks as their nutrient supplement – but most of them are converted the feedstocks by using fungal fermentation process before being used as a primary source of nutrient media for the bacterial fermentation process – They want to break down more to simplify the nutrient
  3. ( Lowenberg et al ., 1999) – Quoted from Teresa’s thesis references
  4. (Lee, 1999; Lee, 1996) - q uoted from this website - http://2008.igem.org/Team:Utah_State/Project
  5. And now, lets look at the properties of this type of microb which is R. eutrophus a.k.a
  6. (Verlinden, 2007) - quoted from this website - http://2008.igem.org/Team:Utah_State/Project
  7. Why are we going to use PHB instead of using other natural polyesters? ( Deng et al., 2002) - Literature MS WORD (Lee et al., 1998; Van et al., 1985) – Teresa’s thesis references
  8. KH 2 PO 4 - Potassium dihydrogen phosphate
  9. Microbial biosynthesis of PHB starts with the condensation of two molecules of acetyl- CoA to give acetoacetyl-CoA which is subsequently reduced to hydroxybutyryl-CoA. This latter compound is then used as a monomer to polymerize PHB