The Effect Of Pseudophosphorylated Tau Protein In The Absence Of Fibrillar Beta Amyloid

The Effect of Pseudophosphorylated Tau Protein in the Absence of Fibrillar Beta-Amyloid By, Robert Dorfman

Purpose ,[object Object],[object Object]

Background ,[object Object],[object Object],[object Object],[object Object],Normal Alzheimer’s

AD Statistics ,[object Object],[object Object],[object Object],[object Object]

Tau Proteins ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Courtesy of JYI.org

NEUROFIBRILLARY DEGENERATION 1 2 3 4 5 6 2 3 4 5 Courtesy of Alonso et al.

Pathogenesis of Alzheimer Disease AD Tau Tangles  -Amyloid Plaques

BACKGROUND ,[object Object],[object Object],[object Object],[object Object],[object Object]

HYPOTHESIS ,[object Object],[object Object],[object Object]

MATERIALS & METHODS ,[object Object],[object Object],EGFP WT normal tau, control EGFP R406W mutated tau, control EGFP 6B Pseudophosphorylated normal tau, variable EGFP 8A Pseudophosphorylated mutated tau, variable

METHODS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

METHODS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Trial #1 - Normal Tau DAPI Tau Caspase-3 Overlay

Trial #1 - Mutated Tau EGFP 8A Tau DAPI Caspase-3 Overlay

Trial #1 - Pseudophosphorylated Normal EGFP 6B DAPI Tau Caspase-3 Overlay

Trial #1 - Pseudophosphorylated Mutated DAPI Tau Caspase-3 Overlay

Trial #2 - Normal Tau Tau DAPI Caspase-3 Overlay

Trial #2 - Mutated Tau DAPI Tau Caspase-3 Overlay

Trial #2 - Pseudophosphorylated Normal Tau DAPI Caspase-3 Overlay

Trial #2 - Pseudophosphorylated Mutated Tau DAPI Caspase-3 Overlay

Percentage of Caspase-3 Activation Trial #1 - % Caspase-3 Activation Trial #2 - % Caspase-3 Activation Pseudo Mutated 85.2 92 Mutated 66 52.3 Normal 0 16.9 Pseudo Normal 79 90

Percentage of Caspase-3 Activation

Statistical Analysis ,[object Object],[object Object],[object Object],Trial #1 – P - Values Trial #2 – P - Values Normal vs. Mutated 8.159 x 10 -9 9.614 × 10 -11 Normal vs. Pseudo Normal 5.900 × 10 -12 0 Normal vs. Pseudo Mutated 0 0

SUMMARY OF RESULTS & CONCLUSIONS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Possible Expansions ,[object Object],[object Object],[object Object],[object Object],[object Object]

SOURCES ,[object Object],[object Object],[object Object],[object Object]

Acknowledgements: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

The Effect Of Pseudophosphorylated Tau Protein In The Absence Of Fibrillar Beta Amyloid

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