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- 1. Adenovirus, parvovirus, polyomavirus www.freelivedoctor.com
- 3. From Medical Microbiology, 5 th ed., Murray, Rosenthal & Pfaller, Mosby Inc., 2005, Fig. 53-1. Adenovirus Structure www.freelivedoctor.com
- 4. E1 E2 E4 E3 Late genes Adenovirus Genome 36 kb linear ds DNA Early genes for host and viral transcription control, viral DNA replication Late genes for virion structure www.freelivedoctor.com
- 5. (From Fields Virology, 4th ed, Knipe & Howley, eds, Lippincott Williams & Wilkins, 2001, Fig. 67-5.) Adenovirus replication cycle www.freelivedoctor.com
- 7. From Medical Microbiology, 5 th ed., Murray, Rosenthal & Pfaller, Mosby Inc., 2005,, Fig. 50-4. Adenovirus pathogenesis www.freelivedoctor.com
- 11. Parvovirus Genome 5 kb ssDNA, inverted terminal repeats (ITR) Rep gene required for DNA replication Cap gene encodes capsid proteins ITR ITR www.freelivedoctor.com Rep Cap
- 12. Autonomous parvovirus replication www.freelivedoctor.com
- 13. Helper dependent parvovirus (AAV) replication Infection without adenovirus Infection with adenovirus Superinfect with adenovirus Lytic replication www.freelivedoctor.com AAV DNA integrates into chromosome 19
- 20. Replication cycle of polyomaviruses. Steps in the replication cycle are indicated by numbers as follows: 1, adsorption of virions to the cell surface; 2, entry by endocytosis; 3, transport to the cell nucleus (route and mechanism not yet known); 4, uncoating; 5, transcription to produce early region mRNAs; 6, translation to produce early proteins (T antigens); 7, viral DNA replication; 8, transcription to produce late region mRNAs; 9, translation to produce late proteins (capsid proteins); 10, assembly of progeny virions in the nucleus; 11, entry of virions into cytoplasmic vesicles (mechanism unknown); 12, release of virions from the cell by fusion of membrane vesicles with the plasma membrane; 13, released virion. Polyomavirus replication www.freelivedoctor.com