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Protein Synthesis
By: Alli Ishman
Nucleus

Cytoplasm
Nucleus
5
’

3’

Phosphate

Sugar (Deoxyribose)
Adenine
Thymine
Guanine
Cytosine
3’ Promoter Region

Coding Region

Phosphate

Sugar (Deoxyribose)
Adenine
Thymine
Guanine
Cytosine

RNA Polymerase

Termination Sequence 5
’
AUGGA

3’

5
’

Phosphate

Sugar (Deoxyribose)
Adenine
Thymine
Guanine

mRNA Strand

Cytosine

RNA Polymerase
AUGGAGAGAAGG

3’

Phosphate

Sugar (Deoxyribose)
Adenine
Thymine
Guanine

mRNA Strand

Cytosine

RNA Polymerase

5
’
5
’

3’

Phosphate

Sugar (Deoxyribose)
Adenine
Thymine
Guanine

mRNA Strand

Cytosine

RNA Polymerase
Cytoplasm

Nuclear Pore
Ribosomes

Ribosomes

Ribosomes
Ribosomes

Ribosomes

Ribosomes
Cytoplasm

Start Codon

Codon

Codon

Sugar (Deoxyribose)
Adenine
Uracil
Guanine
Cytosine

mRNA Strand
Phosphate

Codon

Stop Codon
Ribosome
Large Subunit
Small Subunit

Start Codon
Adenine
Uracil
Guanine
Cytosine

Codon

Codon

tRNA
molecule

Codon
Amino Acid

Ribosome
Anti-codon
mRNA
Strand

Stop Codon
Ribosome
Large Subunit
Small Subunit

Start Codon
Adenine
Uracil
Guanine
Cytosine

Codon

Codon

tRNA
molecule

Codon
Amino Acid

Ribosome
Anti-codon
mRNA
Strand

Stop Codon
Ribosome
Large Subunit
Small Subunit

Polypeptide bond

Start Codon
Adenine
Uracil
Guanine
Cytosine

Codon

Codon

tRNA
molecule

Codon
Amino Acid

Ribosome
Anti-codon
mRNA
Strand

Stop Codon
Ribosome

Large Subunit
Polypeptide bond

Start Codon
Adenine
Uracil
Guanine
Cytosine

Polypeptide bond

Codon

Codon

tRNA
molecule

Small Subunit

Codon
Amino Acid

Ribosome
Anti-codon
mRNA
Strand

Stop Codon
Polypeptide bond

Start Codon
Adenine
Uracil
Guanine
Cytosine

Polypeptide bond

Codon

Codon

tRNA
molecule

Polypeptide bond

Codon
Amino Acid

Ribosome
Anti-codon
mRNA
Strand

Stop Codon
Peptidebond

Start Codon
Adenine

Peptide bond

Codon

Peptide bond

Codon

tRNA
molecule

Peptide bond

Codon
Amino Acid

Uracil
Guanine
Cytosine

Anti-codon
mRNA
Strand

Stop Codon
Polypeptide Chain

Peptide bond

Start Codon

Peptide bond

Codon

Adenine
Uracil
Guanine
Cytosine

mRNA
Strand

Peptide bond

Codon

Peptide bond

Codon

Stop Codon
Primary Structure
Secondary Structure
Tertiary Structure
First DNA transcription takes place in the nucleus of the cell. RNA polymerase
binds to the start of a gene on the double helix and it unwinds the DNA into single
strands. As it goes along it uses one of the single strands as a template and builds a
complementary mRNA strand. RNA polymerase uses RNA instead of DNA.
Therefore, thymine is replaced by uracil. The mRNA breaks off from the template
strand and moves from the nucleus into the cytoplasm through a nuclear pore,
translation begins and DNA returns to its original double-helix formation. Then
mRNA comes into contact with the ribosomes which consist of two parts; a large
subunit and a small subunit. Ribosomes read the mRNA sequence and convert it
into an amino acid sequence. The ribosomes begin translation at the start codon
AUG and in this case, stop at the stop codon UGA. tRNA molecules transfer the
amino acid to the ribosome. tRNA contains an anticodon on one end and an amino
acid on the other. The tRNA with a complementary anticodon temporarily binds
onto the codon, then the ribosome reads the next codon and another tRNA with a
complementary anticodon binds. A peptide bond joins the two amino acids, and as
the ribosome continues down the mRNA strand the first tRNA falls off, leaving its
amino acid. The ribosome continues down the strand until the stop codon is
reached, where the last tRNA falls off, leaving a chain of amino acids, which will
then fold upon itself to give it its function.

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