1. CLINICAL MICROSCOPY/UBF
2ndSemesterA.Y.2010-2011

2. Definition
Clinical Microscopy (MTE07)
• The course deals with the macroscopic,
chemical and microscopic study of the
different non-blood body fluids and their by
products

3. Clinical Microscopy
• urine, feces, bronchial and bronchoalveolar
lavage, sputum, gastric and duodenal
contents, pancreatic exocrine secretions,
cerebrospinal fluid, synovial fluid, serous
fluids (transudates and exudates), sweat,
seminal fluid and amniotic fluid.
• Collection, handling, processing, reference
ranges and clinical significance

4. PRE-REQUISITES:
MICROBIOLGY
PHYSIOLOGY
COURSE CREDIT:
2 units lec; 1 unit lab

5. REQUIREMENTS:
1. Color code: YELLOW (LAB)
ORANGE (LEC)
2. Orange highlighter and
blue ballpen
3. Activity notebook
4. Lecture notes &
compilation of hand-outs
5. Portfolio – deadline (final
exam)
6. Textbook
7. Quota – processing of
specimen and case
report, 5 per person (20%
of FE)
8. Lab: preliminary reports,
post-exam conference,
protocols, data sheets
9. PPE; specimen; Group
materials
10. Conference – prelims
(case ana); SF (quiz show)

6. QA and Safety
• Basic terms
• Quality Assurance
• Laboratory Safety
– Biological hazards
– Sharp hazards
– Chemical hazards
– Radioactive hazards
– Electrical hazards
– Fire/explosive hazards
– Physical hazards

7. Microscopy
• Types of Microscopy
• Brightfield Microscope
• Care and Use of the Microscope

8. Renal Function
• Renal Anatomy
• Renal Blood flow
• Renal Physiology
– Functions
– Processes
• Glomerular Filtration
• Tubular Reabsorption
• Tubular Secretion

9. Urinalysis
• Introduction (History, Urine
formation, composition, volume,
specimen collection, handling)
• Physical examination of urine
• Chemical examination of urine
• Microscopic examination of urine
• Other methods

10. Renal and Renal-Associated Dses
• Glomerular
• Tubular
• Interstitial
• Vascular
• Renal Failure
• Renal lithiasis
• Metabolic disorders

11. Other Exams for Renal Dses
• Basic terms
• Urinary sediment Quantification
• Stone Analysis
• Assessment of GFR
• Concentration tests
• Tubular Secretory fxn tests

12. OBF’s
• Pregnancy Test and HCG Assay
• CSF
• Seminal fluid
• Synovial fluid
• Serous fluid
• Amniotic fluid
• Sweat
• Bronchial washings, bronchoalveolar lavage & sputum
• Gastric fluid, Duodenal content and pancreatic
exocrine secretion
• Fecal analysis

13. References
Brunzel, Nancy A. Fundamentals of Urine and Body Fluid
Analysis. W.B. Saunders, Philadelphia. 1994
Henry, John Bernard et al. Clinical Diagnosis by Laboratory
Methods. 20th Edition, W.B. Saunders, Philadelphia.
2001
Estridge, Barbara et al. Basic Medical Laboratory
Techniques. 4th Edition. Delmar, USA. 2000
Leihmann, Craig A (ed). Saunder's Manual of Clinical
Laboratory Science. W.B. Saunders, Philadelphia. 1998

14. References
McBride, Landy J. Textbook of Urinalysis and Body Fluids: A
Clinical Approach. Lippincott, Philadelphia. 1998
Sacher, Ronald A & McPherson, Richard A. Widmann's
Clinical Interpretation of Laboratory Tests. 11th Edition.
F.A. Davis Company, Thailand. 2000
Strasinger, Susan K. & Di Lorenzo, Marjorie S. Urinalysis
and Body Fluids. 5th Edition. F.A. Davis Company,
Thailand. 2008