1. Cells
•Smallest unit of life.
•Able to carry out all functions of life.
Cell Theory
• All living organisms are made of one or more
cells.
• All cells come from other cells.
2. 2
The Cell Cycle
•series of changes a cell
undergoes from the time
it forms until the time it
divides
• stages
• interphase
• mitosis
• cytoplasmic division
3. Stages of Interphase
3
• Cell grows, maintains routine functions
• Cell replicates genetic material and synthesizes new organelles to prepare for
division
• G1: 1st growth stage. Cell matures and carries out it’s assigned function. May
last indefinitely.
• S: Genes are copied
• G2: 2nd growth stage. Membranes, cytosol, and organelle numbers increase.
5. 5
Genes
• DNA- molecule that carries
genetic information
• Chromosome- DNA plus
associated proteins
• Gene – segment of DNA
that codes for one protein
• Genome – complete set of
genes
1 gene = 1 protein
12. 12
Genes
Gene – segment of DNA that codes for one protein
1 gene = 1 protein
Genome – complete set of genes
Genetic Code – method used to translate a
sequence of nucleotides of DNA into a sequence of
amino acids
What is the difference between a gene, DNA, and a
chromosome?
13. Protein Synthesis
13
1) Transcription: DNA copied to mRNA in
nucleus
2) Translation: mRNA copied to protein at
ribosomes
•Codon: mRNA triplet
•Anticodon: tRNA triplet
DNA mRNA protein
14. DNA vs RNA
DNA RNA
double stranded single stranded
Deoxyribose Ribose
A,C,T,G A,C,U,G
Must stay in nucleus Can leave nucleus
1 type 3 types
15. 15
RNA Molecules
Messenger RNA (mRNA) -
• delivers genetic information from nucleus to the cytoplasm
• single polynucleotide chain
• formed beside a strand of DNA
• RNA nucleotides are complementary to DNA nucleotides
(exception – no thymine in RNA; replaced with uracil)
• making of mRNA (copying of DNA) is transcription
Transfer RNA (tRNA) -
• carries amino acids to mRNA
• carries anticodon to mRNA
• translates a codon of mRNA into an amino acid
Ribosomal RNA (rRNA) –
• provides structure and enzyme activity for ribosomes
22. Protein Chain
•Where did the
original DNA go?
•Where did the
mRNA go?
•Where did the
tRNA go?
•Where did the
ribosome go?
•Where did the
amino acids go?
•Where does the
protein go?
Interphase is the phase when the cell is not dividing. Mitosis is the act of dividing. Interphase is divided into 3 sections. During G1 phase it lasts for a few hours or a 100 years, it depends on the type of cell. Skin cells divides quickly nerve cells divide over many years… Once you cross the G1 phase line you are having babies, During the Sphase we are copying DNA so we have enough for both new cells, G2 phase we are further separating cells into two growing all that stuff in cells in preparation for division. Next is the phase of mitosis the final division of one cell into two.
S phase nothing changes but the amount of DNA in the nucleous. In the G2 phase everything else in the cytoplasm gets duplicated, then it goes through mitosis and splits into two cells
Parent cell goes through the whole process then creates daughter cells, the two daughter cells are identical to eachother and the parent. This happens in what is called sematic cell, which is anything that is not an egg or sperm. We do this for growth as well as repair, or to replace things. The lining of the digestion trac gets replaced quite often. Do a paper on Psoriasis in relation to cell mitosis..
Chromosomes are double stranded – molecules across the top and bottom, held together by covalent bonds. Hydrogen bonds are weak bonds in the center. If you add tension to it it will just unzip… The structure has a lot to do with how it functions. 4 letter ATGC make up the DNA C’s always pair with G’s and A’s always pair with T’s cause they fit together and it wobbles. When you wrpa it up with protein (the DNA) it makes a double helix.
In Sphase I want to make more DNA and enzyme come in and separates the DNA at the weak bonds and another DNA starts to form until we have two new strands.
This can keep going on and on Semi conservative means half old half new.
Compares the 2 new copies to eachother and corrects it These are going along like a billion letters a second, so we have an editing enzyme that checks the DNA letters comparing the copies. A change in the DNA is a mutation… Plasma is the water in the blood FYI
G1 phase has un unduplicated dna strand
TAC CAA ACC GAG GTC ATG GTT TGG CTC CAG On the quiz I will have to fill in the second line like I did it here this is the complementary line…Genetic Code is like the rosetta stone for making protein, its how you translate form DNA language that has 4 letters to protein that has 20 letters. We get the DNA building blocks from the food we eat…
Ribosomes are protein factories inside the cellCodon:Anti codon: DNA replication happens only in the Sphose, but protein syntheses happens during the whole process. This is a two step process transcription copies the DNA, proteins are made in ribosomes that are outside of the nucleus. Make a copy of DNA onto RNA and take it to the ribosome for protein synthesis. During Translation we take the RNA and make it into a new protein. Codon is trhee letters on an mRNA and Anticodon is a three letter on the tRNA
In dna there ACTG Rna +ACUGDna is two stranded but RNA is single stranded. DNA has sugar calleddeoxyribose and RNA has sugar called Ribose hense the names… D for DNA R for RNA There are 2 kinds of RNA
Photocopy of the geneproteins are made out of amino acidsRrna- is the rnaMRNA is the photocopy, rRNA is the structure, it can go in and out of the nucleus, and is made in the nucleolus., tRNA are like taxis that carry amino acids back and forth and amino acids are what proteins are made of.
Stage 1 of protein synthesis, This enzyme is a RNA sugar
mRNA comes in and splits the DNA copies it and takes a single strand out to the ribosomes for protein synthesis, so wherever there was a T we now have a U cause T’s don’t exist anymoreTAC CAA ACC GAG GTCAUG GUU UGC CUC CAG the complementary mRNA strand … DNA is the blueprint to make the protein…
2nd step, mRNA comes into the ribosome pink block is thetRNA the taxi carried food proteins into the ribosome to connect to the mRNA to form the new protein strand. The mRNA just keeps going into another ribosome to create more protein…
All of the amino acides, blue blocks go into the protein strand. Protein then goes everywhere all over the body wherever needed, hair, organells, organs, muscles, etc…
Ribosomes- stay until you are ready to use againAmino acids- in the proteinPolypeptide- goes wherever it needs to*Everything gets used again except the amino acids which are synthesized with in the protein
Diversity equals stability, Redundancy is the same as diversity for resiliency… We have our mRNA and we then match it to our tRNADNA -TAC CAA ACG GAC GTCmRNA – AUG GUU UGC CUC CAGtRNA – UAC CAA ACG GAG GUCAas (amino acid) is read from the mRNA line so AUG equals met, GUU val, UGC cys, CUC leu