5. Summary of transplantation assays – total BM or SP cells despite more than 10 times less frequency of HSCs in spleen, their activity is equal to BM counterparts Parameter BM SP % chimerism (1M donor+1M competitor) 50% 6.5% RU value per 1M donor cells 11.2 0.75 Repopulation pattern multilineage lymphoid-biased LTRCs frequency 1 in 30k 1 in 310k Mean activity per stem cell MAS = RU/CRU 0.34 0.39
6. Summary of in vitro and in vivo assays – sorted HSCs (LSK/CD34-) repopulating and self-renewal potentials of HSCs are similar in BM and spleen Parameter BM SP % chimerism (10 HSCs + 0.2M competitor) 16.7% 9% % chimerism in 2 nd transplant (whole pooled cells) 12.3 9.8 Single HSC transplant positive/ total 9/29 7/29 LTRC frequency roughly estimated 1 in 3 1 in 5 Repopulation pattern multilineage multilineage
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