3 Ways to Obliterate Bullet Points From Your Slides

1. awesome
presentations
newsletter
!
slide
makeover 2

2. My#Role#on#the#Project#
/ Tes2ng#an2bodies#
/ Determining#sensi2vity#of#IRIS#for#pseudotyped#VSV#detec2on#
/ Determining#repeatability#
/ Valida2ng#new#IRIS#instruments#(IRIS#on#med#campus)#
/ Standardizing#the#chip#prepara2on/incuba2on#protocol##
/ To#improve#sensi2vity#and#repeatability#with#surface#op2miza2on#

3. Hi, my name is Collaborator X and
I'm responsible for optimizing the
protocol that we use to coat the
chips with antibodies. My job is to
make sure that each chip has as
few foreign particles as possible,
and that each antibody has a high
affinity for the virus we are trying to
detect. After incubating the chips, I
scan them and analyze the data.
Then, I try to come up with ways to
lower the variability and improve the
sensitivity of our results.
“

4. Read the full story
behind this
slide makeover

5. Chip%Prepara*on%Protocol%
Chip%Spo(ng%&%Washing:%
%
0%An*body%solu*ons%are%spo7ed%at%305%mg/ml%in%PBS%with%50%mM%Trehalose.%%%
%%
0%ADer%overnight%incuba*on%in%the%spo7er%(at%60%%humidity),%chips%are%washed%in%
PBST%for%30%minutes,%rinsed%in%1X%PBS%for%3%minutes%and%dI%for%3%minutes%and%dried%
with%argon.%
Incuba4on%of%Chips%with%Concentrated%Samples:%
!
0%Chips%are%scanned%on%IRIS%for%pre0incuba*on%par*cle%counts.%
%%
0%Chips%are%incubated%with%1%ml%of%the%virus%sample%for%2%hours%on%shaker.%%
0%ADer%incuba*on%ends,%chips%are%washed%in%1X%PBS%for%3%*mes,%each%1%min%and%then%
0.1X%PBS%for%1%min,%dipped%in%dI%for%10%seconds%and%pulled%out%of%the%water%slowly%to%let%
the%water%slide%down%the%chip.%
%%
0%Chips%are%scanned%with%IRIS%for%analysis.%

6. Spotting and Washing Pre-incubation scan
Incubation with virus Post-incubation scan
500
520
540
560
580
600
LED
LED

7. Future&Work&
* Dilu.on&experiment&for&Marburg*pseudotyped&VSV&
* 10^4&PFU/ml&detec.on&in&30&minutes&
&
* Tes.ng&of&New&Lassa&An.bodies&(7&of&them)&&&
* Dilu.on&curve&for&Lassa*pseudotyped&VSV&for&LOD&determina.on&

8. Obtain dilution curves for!
Marburg- and Lassa-pseudotype VSV

9. Obtain dilution curves for!
Marburg- and Lassa-pseudotype VSV
Try to detect viruses at lower !
concentrations (104 PFU/ml) after !
shorter incubation periods (30 minutes)

10. Obtain dilution curves for!
Marburg- and Lassa-pseudotype VSV
Try to detect viruses at lower !
concentrations (104 PFU/ml) after !
shorter incubation periods (30 minutes)
Test 7 new Lassa antibodies

13. Click on the envelope
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the next slide makeover
(probably on sunday)