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Water, pH and Buffers
Objectives ,[object Object],[object Object],[object Object],[object Object]
Water: Structure and Properties ,[object Object],[object Object],[object Object],[object Object],[object Object]
Water is a dipolar Molecule The approximate shape and charge distribution of water.  Martin Chaplin, http://www.lsbu.ac.uk/water/molecule.html The two hydrogen atoms have partial positive ( δ + ) charges and the oxygen molecule has a partial negative (2 δ - ) charge.  The result of unequal sharing is two electrical dipoles, one along each H-O bond.
H-Bonding between water molecules ,[object Object],[object Object],http://courses.cm.utexas.edu/jrobertus/ch339k/overheads-1.htm ,[object Object]
Water is found in 3 forms on planet Earth ,[object Object],[object Object],Gas  Liquid  Solid
Boiling and freezing point of water
Water: Cohesion and Adhesion ,[object Object],[object Object],http://www.martin-waugh.com/wp-content/uploads/2007/08/water-drop-1b.jpg
Solubility of Molecules in Water  ,[object Object],[object Object],[object Object],Hemoglobin
Heme group (active site) in cytochrome f
 
Amphipathic Compounds ,[object Object],[object Object]
[object Object],[object Object],[object Object]
Weak Interactions among Biomolecules in Aqueous Solvent ,[object Object],[object Object],[object Object],[object Object],[object Object]
Colligative properties of aqueous solutions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Osmotic Pressure ,[object Object],[object Object],[object Object],[object Object]
Effect of extracellular osmolarity on  water movement across a  plasma membrane Effect of extracellular osmolarity on  water movement across a  man-made, selectively permeable membrane
Ionization of water ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Ionization of water
[object Object],[object Object],[object Object],Ionization of water
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Acids, Bases and Buffers ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Strong acids and strong bases ,[object Object],[object Object],[object Object]
Weak Acids and Bases ,[object Object],[object Object],[object Object],[object Object]
Dissociation Constant ,[object Object],[object Object],[object Object],[object Object],[object Object]
pH, Acids and Buffers ,[object Object],[object Object],[object Object],[object Object]
Titration Curve for Acetic Acid and its Conjugate Base NaOH is added incrementally to HAc, and the pH of the mixture is measured.  This value is plotted against the amount of NaOH expressed as the fraction of total NaOH required to convert all the HAc to its protonated form.  At the midpoint of titration, the pH is equal to the pKa. The shaded zone is the region useful for buffering power, generally between 10% and 90% titration of a weak acid.
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The Hendersson-Hasselbach Equation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Example Problems ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Weak acids or bases buffer cells and tissues against pH changes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Titration curve for the carbonic-acid-bicarbonate buffer. Note that the pH of the blood (7.4) lies outside the region of greatest buffering capacity (green).
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
 

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Water And Buffers

  • 1. Water, pH and Buffers
  • 2.
  • 3.
  • 4. Water is a dipolar Molecule The approximate shape and charge distribution of water. Martin Chaplin, http://www.lsbu.ac.uk/water/molecule.html The two hydrogen atoms have partial positive ( δ + ) charges and the oxygen molecule has a partial negative (2 δ - ) charge. The result of unequal sharing is two electrical dipoles, one along each H-O bond.
  • 5.
  • 6.
  • 7. Boiling and freezing point of water
  • 8.
  • 9.
  • 10. Heme group (active site) in cytochrome f
  • 11.  
  • 12.
  • 13.
  • 14.
  • 15.
  • 16.
  • 17. Effect of extracellular osmolarity on water movement across a plasma membrane Effect of extracellular osmolarity on water movement across a man-made, selectively permeable membrane
  • 18.
  • 19.
  • 20.
  • 21.
  • 22.
  • 23.
  • 24.
  • 25.
  • 26.
  • 27. Titration Curve for Acetic Acid and its Conjugate Base NaOH is added incrementally to HAc, and the pH of the mixture is measured. This value is plotted against the amount of NaOH expressed as the fraction of total NaOH required to convert all the HAc to its protonated form. At the midpoint of titration, the pH is equal to the pKa. The shaded zone is the region useful for buffering power, generally between 10% and 90% titration of a weak acid.
  • 28.
  • 29.
  • 30.
  • 31.
  • 32.
  • 33.
  • 34.