Affinity chromatography 1

Affinity Chromatography and Ion Exchange Chromatography Shannon E. Spence

What on Earth did scientist do before Chromatography? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],www.chemguide.co.uk /.../ idealfract.html

What entices the scientists to Chromatography? ,[object Object],[object Object],[object Object],[object Object],[object Object],http://www.residues.com/ion_chromatography.html

Brief History of Chromatography ,[object Object],[object Object],[object Object],[object Object],http://www.chemgeo.uni-hd.de/texte/kuhn.html

History of the Main techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],British chemist Archer John Porter Martin, co-recipient, with Richard L. M. Synge, of the 1952 Nobel Prize in chemistry, "for their invention of partition chromatography." http://www.chemistryexplained.com/Ma-Na/Martin-Archer-John-Porter.html

History of the Main Techniques ,[object Object],[object Object],[object Object],[object Object],library.thinkquest.org

Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object]

Principles of Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

More Detailed History of IE Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Terminology ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Ion-Exchange Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

How do you get those columns to work ,[object Object],[object Object],[object Object]

Resin ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Cation Exchange resins ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Anion Exchange Resins ,[object Object],[object Object],[object Object],[object Object],[object Object]

To Affinity and Beyond… ,[object Object],[object Object],[object Object]

What makes it unique… ,[object Object],[object Object],[object Object],[object Object],[object Object]

Relative Affinity of Ions ,[object Object],[object Object],[object Object],[object Object]

Ion Exchange Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Applications of IEC ,[object Object],[object Object]

Ion Chromatography ,[object Object],[object Object]

Affinity History ,[object Object],[object Object],[object Object],[object Object],[object Object]

How do they get those iddy bitty molecules in there?

So now what…. ,[object Object],[object Object],[object Object],[object Object]

Specificity of Affinity Chromatography ,[object Object],[object Object],[object Object],[object Object]

Matrix ,[object Object],[object Object],[object Object]

Ligand ,[object Object],[object Object],[object Object],[object Object],[object Object]

So many ligands so little time ,[object Object],[object Object],[object Object],[object Object]

Applications ,[object Object],[object Object],[object Object]

Gas Chromatography Gas-Liquid Chromatography And Gas-Solid Chromatography

History ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Gas Chromatography ,[object Object],[object Object],[object Object],[object Object]

Instrumentation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],http://teaching.shu.ac.uk/hwb/chemistry/tutorials/chrom/gaschrm.htm

Carrier Gas ,[object Object],[object Object],[object Object],[object Object]

Sample injection port ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Columns ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Column ,[object Object],[object Object]

Column Temperature ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Detectors ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Flame Ionization Detector (FID) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Applications ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Mass Spectrometry ,[object Object],[object Object],http://www.chem.arizona.edu/massspec/intro_html/intro.html

Instrumentation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Operation of Instrument ,[object Object],[object Object],[object Object],[object Object],[object Object]

History ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

History of Modern Day MS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Mass Spectrometry ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Applications ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Great Scott, it can be used in Biochemistry too! ,[object Object],[object Object],[object Object],[object Object],[object Object]

Mass spectrometers can be divided into three fundamental parts. ,[object Object],[object Object],[object Object]

Ionization Source ,[object Object],[object Object],[object Object],[object Object]

Analyzer ,[object Object],[object Object]

Sample Introduction ,[object Object],[object Object],[object Object],[object Object]

Ionization Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Ionization Methods Soft method very high mass to 500,000 Daltons Sample mixed in solid matrix Peptides Proteins Nucleotides Matrix Assisted Laser Desorption (MALDI) Soft method but harder than ESI or MALDI to 6,000 Daltons Sample mixed in viscous matrix Carbohydrates Organometallics Peptides nonvolatile Fast Atom Bombardment (FAB) Soft method ions often multiply charged to 200,000 Daltons Liquid Chromatography or syringe Peptides Proteins nonvolatile Electrospray (ESI) Soft method molecular ion peak [M+H]+ to 1,000 Daltons GC or liquid/solid probe Relatively small volatile Chemical Ionization (CI) Hard method versatile provides structure info to 1,000 Daltons GC or liquid/solid probe Relatively small volatile Electron Impact (EI) Method Highlights Mass Range Sample Introduction Typical Analytes Ionization method

Electrospray Ionization (ESI) ,[object Object],[object Object]

Electrospray Ionization (ESI) ,[object Object]

Electron Impact (EI) ,[object Object],[object Object],[object Object],[object Object]

Analyzers ,[object Object],[object Object],[object Object]

Analyzers ,[object Object],[object Object],[object Object],[object Object],[object Object]

Detectors ,[object Object],[object Object],[object Object]

Key Terminology ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Interpreting Spectra ,[object Object]

[object Object],[object Object]

References ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Affinity chromatography 1

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Affinity chromatography 1