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Aug 26 2011
1.
BT-202 Netaji Subhas
Institute of Technology, Dwarka, New Delhi. Dr. Amita Pandey Aug 26, 2011
2.
3.
4.
5.
Structure of proteins
Proteins perform diverse functions in the cell.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
peptide fragments Sequencing
Edman procedure Ordering of peptides Cleaved again with different enzyme or chemical
17.
18.
19.
20.
Genome sequencing organism
Size of genome Year Haemophilus 1.8 Mb 1995 Saccharomyces 12.1 Mb 1996 C. elegans 100Mb 1998 Fruit fly 139 Mb 2005 Human 3.5 Gb 2006
21.
Consensus sequences
22.
Synthesis of peptides
Three ways to obtain a peptide are -from the tissue -genetic engineering -chemical synthesis
23.
Chemical synthesis 9-fluorenylmethoxycarbonyl
24.
25.
26.
27.
Non-covalent bonds in
protein folding
28.
Globular protein
29.
Hydrogen Bonds in
Proteins
30.
31.
32.
Protein folding
-helix – protein turns like a spiral – fibrous proteins (hair, nails, horns) -sheet – protein folds back on itself as in a ribbon –globular protein
33.
34.
35.
Protein structure
36.
37.
Domains
38.
39.
40.
Single Subunit Proteins
41.
42.
43.
44.
45.
Collagen and Elastin
46.
47.
48.
Ligand Binding
49.
50.
51.
52.
Antibodies
53.
54.
55.
56.
57.
58.
Features of Enzyme
Catalysis
59.
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