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GENERAL SEMINAR TOPIC: IMPORTANCE OF FASTA AS A BIOINFORMATIC TOOL PRESENTED BY: MAYANK SRIVASTAVA

INTRODUCTION ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

HEURISTIC METHODS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],6 5 5 5 5 4 3 3 3 2 1 A 5 5 5 5 4 4 3 3 2 2 1 G 4 4 4 4 4 4 3 3 2 2 1 C 3 3 3 3 3 3 3 3 2 2 1 T 2 2 2 2 2 2 2 2 2 2 1 A 2 2 2 2 1 1 1 1 1 1 1 G 1 1 1 1 1 1 1 1 1 1 1 G A T T G A C T T A A G

[object Object],FASTA focus on this area

[object Object],[object Object],[object Object],Look-up Table Dot—Matrix 1 2 3 4 5 6 7 8 9 10 11 4,5,9,10 T 1,8 G 6 C 2,3,7,11 A Location Q * * * * A * * G * C * * * * T * * * * A * * G * * G A T T G A C T T A A G

FASTA ALGORITHM ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

FASTA Steps 1 Local regions of identity are found Different offset values Identical offset values in a contiguous sequence 2 Rescore the local regions using PAM or Blos. matrix Diagonals are extended 3 Eliminate short diagonals below a cutoff score 4 Create a gapped alignment in a narrow segment and then perform S-W alignment

VERSIONS OF FASTA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Application ,[object Object],[object Object]

BIBLIOGRAPHY ,[object Object],[object Object],[object Object]

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