1. Specialty Chemical
Manufacturing Expertise
Since 1928
GFS Chemicals
Chromatography
Products
2006-2007 PRODUCT CATALOG

2. Products For
Chromatography
And Material
Separation/
Purification
The GFS tradition of personal • Specialty Silicas, • Florisil®, Ion Exchange
service and attention to detail Unmodified, 40, 60, 90, and Resins (p. 134-135)
has attracted the attention of 150 Angstrom, GFSelectTM • Drierite®, Activated
experts in chromatography grade (page 130), Bio- Carbons, Diatomaceous
who have long seen a need for refinedTM qualified by Earths (page 136)
CHROMATOGRAPHY
this type of commitment to RNase, DNase and Protease • Specialty Reagents for
problem solving in separation testing. Chromatography (page 137)
sciences. Our alignment with • Aluminas (Brockmann I: • Bio-RefinedTM Products
the entrepreneurial resources Acidic, Basic, and Neutral); (page 138)
of Diazem Corp. (Midland, MI) W. R. Grace silicas (page • Royer® Anion Exchange
has already provided industry 131-132) Resins and Precious Metal
with practical solutions to • Silica Gel Desiccants; Catalysts (page 139)
environmental and process Molecular Sieves (page 133)
problems. We invite you to
talk to us about using this Diazem MCAC
portfolio of novel technolo-
Flow through a Stainless
gies to address your materials Removal of Pt steel Column of an
recovery or separations from a fluid stream aqueous solution of Pt
(0.01M) gave capture of
issues (note example at right). 0.029g of Pt on 0.3g of
media which is 9.9% Pt
• Custom Modified Silicas for relative to the mass of
the media.
Tough Separations
Problems (page 124) Measurement of the
effluent showed less than
Diazem/Dow tailored 200ppb Pt at the baseline
technologies for the effluent.
• HPLC Solvents (page 126)
• Macherey-Nagel
NUCLEOSIL®, NUCLEODUR®,
unmodified spherical and
irregular silica gels (page
127-130) Plus access to the
27 36 45
full Macherey-Nagel Line of 0 9 18
mL of 0.1M Pt
TLC and Column
Chromatography Products

3. Silicas for Advanced The Vision The GFS Connection
Separations from Diazem Diazem and the Dow Chemical The GFS commitment to offer
Technologies Co. have announced a joint several prominent lines of sili-
All the chromatographic sili- technology and licensing cas, including Macherey-Nagel
cas on the following pages are agreement that will provide and W. R. Grace products,
capable of being modified by the bio-tech market with spe- gives separations scientists a
technology recently patented cialty chromatographic broad base of qualified prod-
by Diazem Corp. The resulting sorbents and engineering ucts, traceable by lot analysis
products frequently allow processes. This includes a under ISO-certification, which
chromatographic separations critical range of state-of-the- may ultimately be enhanced
that are unattainable by stan- art separation capabilities by Diazem/Dow technology.
dard silica media. This novel suitable for geometric and Your customized project can
silica/silane surface chemistry optical isomeric molecules, be managed from research to
CHROMATOGRAPHY
exhibits a utility and, more peptides and proteins. This commercialization with com-
importantly, a predictability in technology will impact the plete confidence in the consis-
solving real-life problems most challenging issues of tency of quality of the sorbent
faced in separation science. analytical and preparative of your choice. GFS technical
chromatography, and address and product support both
industry needs from drug dis- help provide a new gateway
The Technology
covery to commercialization. to problem solving in cutting-
Diazem’s Dual ZoneTM Material
edge separation sciences.
technology uses pore-diffu-
sion control silylation with Unique Diazem Products
two (or more) silane bonding Despite the customized
agents to give selective bond- nature of Diazem products,
ing of one chemical composi- GFS offers a selection of C-18
tion on the outer zone of a Diazem modified silicas for
porous material and a differ- your consideration. These
ent chemical composition on include substrates with poly-
the inner zone. Each zone meric/hydrophilic character
then has a different chemical useful in pharma-biotech sep-
reactivity, and the process arations. New Diazem metal
also allows for better silanol chelating media are also avail-
control. Sorbents tailored via able from GFS. Please check in
this technology can separate the alphabetical section under
diverse materials, especially “Diazem.”
biological substrates, more
completely and efficiently
than standard separations
media.

4. New Diazem Corporation These resins have also shown • Recovery of precious metals
Chelation Technology the ability to remove radioac- • Removal of metals that
Diazem Corporation’s new tive radium from a contami- could otherwise interfere
Chelation adsorbent contin- nated source. The Diazem with analytical procedures
ues to show promise in sever- MCAC phase reduced the • Purification of product or
al markets. radium levels from 15 pico product streams
curies to less than 1 pico • Removal or recovery of
The new materials, referred to curie. Mercury levels were organic and inorganic acid
as “metal chelation and acid reduced to below ppb levels. contaminants
capture” (MCAC) phases, are • Separation of proteins,
based on the immobilization The MCAC phases are an peptides, or nucleotides
of hyperbranched macromole- important extension of • Removal of anion contami-
cules on various support Diazem’s unique, proprietary nants such as phosphates
Dual ZoneTM Materials
CHROMATOGRAPHY
media. These phases offer
the advantage of effectively Technology. In Dual ZoneTM Diazem Corporation has taken
removing metal cation and Materials, silanes are chemi- classical silane, surface sci-
anion contamination from cally bonded to porous silica ence and polymer chemistries
chemical processes, waste- support media by precisely and used them in unconven-
water discharge, and drinking controlling coverage density, tional ways to address market
water. In addition, the materi- silanol capture and phase needs. Feel free to contact
als are very effective acid polarity. Dual Zone Materials GFS regarding possible appli-
scavengers. Applications can be chemically tailored to cations of interest to you.
include removal of Fe, Co, Ni, achieve a range of separation
Cu, Zn, Pt, Pd, Pb, and Hg. Pt gradients for a variety of com-
removal is carried out at a plex separations.
rate up to 20% relative to the
weight of the media. Anions can be removed with
similar efficiency by using
Diazem Anion Capture Phase.
The possible uses of these
materials include:
• Recovery of transition-metal
complexes used in homoge-
neous catalytic processes
• Treatment of waste streams
to remove low levels of
toxic anions

5. HPLC & Specialty
Solvents
GFS has decades of experi-
ence with chemical distilla-
tions, both organic and inor-
ganic. Our growing experience
with the Farchan Laboratories
CHROMATOGRAPHY
line of acetylenic and olefinic
research intermediates has
given us even more insights
into the purification of
volatile, moisture and temper- Each of our specialty solvents capillary gas chromatography
ature sensitive materials. The is carefully analyzed and certi- with electron capture and
grades of solvent offered in fied to meet our published flame ionization detectors.
this catalog cover a full range specifications. The quality of
of applications and sensitivi- each solvent far exceeds Ultra Low Cut-off, Distilled in
ties. These include products reagent specifications set by Glass Solvents — consistently
that are distilled-in-glass or the American Chemical pure solvents for:
quartz, and HPLC solvents Society. Quality is controlled • HPLC
where the emphasis is on not only by UV Spectro- • Gas Chromatography
High Performance. photometry, but also by • Pesticide Residue Analysis
• Spectrophotometry
Min Assay % (by GLC) Max Water Max UV Cutoff Max Residue
ACETONE 99.7 0.50% 330nm 3ppm
ACETONITRILE 99.7 0.02% 190nm 3ppm
CHLOROFORM 99.7 0.02% 245nm 3ppm
CYCLOHEXANE 98.5 0.01% 195nm 3ppm
DICHLOROMETHANE 99.8 0.01% 233nm 3ppm
ETHYL ACETATE 99.6 0.05% 256nm 3ppm
ETHYL ETHER 99.5 0.08% 218nm 3ppm
FORMIC ACID 88.0 N/A N/A 1ppm
HEPTANE 99.5 0.01% 200nm 3ppm
HEXANE ___ 0.01% 200nm 3ppm
METHANOL 99.8 0.08% 205nm 3ppm
PENTANE 98.5 0.01% 195nm 3ppm
PETROLEUM ETHER ___ 0.02% ____ 3ppm
ISOPROPYL ALCOHOL 99.5 0.10% 205nm 3ppm
TETRAHYDROFURAN 99.7 0.03% 215nm 3ppm
TOLUENE 99.7 0.03% 284nm 3ppm
2,2,4-TRIMETHYLPENTANE 99.7 0.02% 210nm 3ppm

6. New Math For
Silica Gel and
Chromatography
Products
MACHEREY-NAGEL...
90+Years in Products for
Chromatography Markets.
GFS CHEMICALS 70+ Years
Serving Analytical and
CHROMATOGRAPHY
Research Markets
It Adds Up...Partners with
160+ years of combined expe-
rience, just a phone call away.
GFS is your gateway to a host
of chromatography products
from Macherey-Nagel GmbH, a
leader in the development of
media and other products for
separation sciences for over
90 years. GFS stocks a wide Larger quantities of these their consistent and well-
range of M-N unmodified materials are also available. defined character:
media, including such popular Please let us know how we
trademarked brands as can help you. • High bed stability due to
NUCLEOSIL®, NUCLEODUR®, spherical particle shape
Polygosil®, and Polygoprep®. NUCLEOSIL® Products – • High efficiency due to
When your needs extend to narrow particle size
the Industry Leader
modified silicas, cellulose, and NUCLEOSIL® is a group of distribution
TLC products derived from totally porous spherical sili- • High separation perform-
the list shown below, GFS can cas, featuring very pure, uni- ance due to optimized
also provide you with those form SiO2 structure distrib- bonding properties
materials in a timely manner. • High chemical and
uted within a small (10 micron
mechanical stability
and finer), narrow particle
Please refer to the following • High load capacity and
size range. Their outstanding
tables and the alphabetical recovery rates
performance in analytical as
section for the core GFS prod- • High reproducibility from
well as preparative column
ucts from Macherey-Nagel. lot to lot
chromatography stems from

7. All NUCLEOSIL® narrow-pore NUCLEODUR® describes a As the name implies, the dura-
packings are stable to 8,500 new group of spherical silicas bility of this collection of sili-
psi; wide bore packings are having average particle size cas is one of its primary fea-
stable to greater than 4,000 falling in ranges greater than tures. The new particle size
psi. This means there are no 10 microns. It takes the place ranges allow for greater sensi-
limitations to the HPLC appli- of the NUCLEOPREP® group, tivity and versatility in its
cations that can benefit from having the advantage of a broad range of applications.
the NUCLEOSIL® product greater selection of narrow While the NUCLEOSIL® line of
group. particle sizes for the 100 finer spherical silicas is espe-
micron pore size: cially recommended for
adsorption chromatography,
SPHERICAL SILICAS the NUCLEODUR® line is also
GFS # M-N Code Description well suited to partition chro-
(angstrom - micron) IRREGULAR SILICAS
matography.
CHROMATOGRAPHY
2829 712000 Nucleosil 50-3 GFS # M-N Code Description
2830 712010 Nucleosil 50-5 (angstrom - micron)
2831 712020 Nucleosil 50-7 2862 711010 Polygosil 60-5 GFS# NUCLEODUR®
2832 712030 Nucleosil 50-10 2863 711280 Polygosil 60-7 2853 713610 Nucleodur 100-10
2864 711020 Polygosil 60-10
2854 713620 Nucleodur 100-16
2833 712360 Nucleosil 100-3
2865 711510 Polygosil 100-5
2855 713600 Nucleodur 100-20
2834 712040 Nucleosil 100-5
2835 712050 Nucleosil 100-7 2866 711520 Polygosil 100-7 2856 713630 Nucleodur 100-30
2836 712060 Nucleosil 100-10 2867 711530 Polygosil 100-10 2857 713551 Nucleodur 100-50
2837 712390 Nucleosil 120-3 2868 711600 Polygosil 300-7
2838 712400 Nucleosil 120-5
2839 712410 Nucleosil 120-7 2869 711890 Polygosil 1000-7
2840 712420 Nucleosil 120-10 The new NUCLEODUR® C18
2870 711001 Polygoprep 60-12 Gravity silica support and col-
2841 712430 Nucleosil 300-5 2871 711240 Polygoprep 60-20 umn packing exhibits excel-
2842 712440 Nucleosil 300-7 2872 711250 Polygoprep 60-30
2843 712450 Nucleosil 300-10 2873 711260 Polygoprep 60-50 lent long-term stability and
2874 711270 Polygoprep 60-80 column performance at low
2844 712680 Nucleosil 500-5 2875 711037 Polygoprep 60-130 and high pH levels. It is espe-
2845 712690 Nucleosil 500-7
2846 712700 Nucleosil 500-10 2876 711002 Polygoprep 100-12 cially well suited for method
2877 711003 Polygoprep 100-20 development because of its
2847 712710 Nucleosil 1000-5 2878 711540 Polygoprep 100-30 robust character and repro-
2848 712730 Nucleosil 1000-10 2879 711550 Polygoprep 100-50
2880 711033 Polygoprep 100-80
ducibility in RP-HPLC. Call or
2849 712850 Nucleosil 4000-5 2881 711034 Polygoprep 100-130 e-mail for a detailed technical
2850 712860 Nucleosil 4000-7 data sheet; please also inquire
2851 712870 Nucleosil 4000-10 2882 711004 Polygoprep 300-12
2883 711610 Polygoprep 300-20
regarding the availability of
2884 711620 Polygoprep 300-30 additional grades of NUCLEO-
2885 711630 Polygoprep 300-50 DUR® having larger pore sizes.
2886 711035 Polygoprep 1000-12
2887 711036 Polygoprep 1000-20
2888 711005 Polygoprep 1000-30
2889 711006 Polygoprep 1000-50

8. STANDARD SILICAS plates and sheets manufac-
(60 Angstrom) tured from exactly the same
grades (per your choice) of
for Low Pressure
silica or cellulose. Their range
Column Chromatography of ready-to-use layers pro-
and Thin Layer vides an added measure of
Chromatography versatility to your problem-
solving arsenal of resources.
TLC Layers Macherey-Nagel silicas can be
used immediately as received
One of the advantages of – no tedious coating or
working with Macherey-Nagel impregnation processes are
TLC grades of silica (60 needed. These TLC plates
Angstrom, below), and cellu- have homogeneous, smooth,
CHROMATOGRAPHY
lose (below) is the assurance well-adhering layers that
that you also have prompt ensure reproducible, quantita-
access to high quality TLC tive evaluations.
Standard silica plates feature
GFS # M-N Code Description
(CC) silica particles between 5 and
2825 815380 230-400 mesh 17 microns. The thickness of
2826 815390 130-270 mesh the silica layer is 0.25 mm for
2827 815320 70-270 mesh analytical plates, and
2828 815330 70-230 mesh typically 0.5mm or 1mm thick
(TLC)
for preparative TLC layers.
2819 816330 5-25 micron
2820 816310 5-25 micron (GB) Most forms are available with
2821 816340 5-25 micron (FI) or without fluorescent indica-
2822 816410 5-25 micron (GB, FI) tor; this includes polyester
2823 816410HP 2-25 micron sheets, aluminum sheets, and
2824 816430HP 2-25 micron (GB) glass plates. Additional infor-
mation on these and other
TLC products is available
CELLULOSE - unmodified powders for TLC from the Macherey-Nagel
Chromatography Guide. All
GFS # M-N Code Description these products are available
through GFS.
2890 816250 MN grade 301 (native, fibrous)
2891 816260 MN grade 301 UV254 (with indicator)
2892 816270 MN 301 HR (acid washed, de-fatted)
2893 815050 MN 100 (native, fibrous, purified)
2894 815060 MN 2100 (high purity grade)
2895 815070 MN 2100ff (high purity, de-fatted)
2896 815290 Avicel® (microcrystalline)

9. TM
HPTLC Layers C-18 Silica GFSelect Silicas purity, acid washed, irregular
silicas having pore sizes of 40,
Reversed Phase Plates
for Chromatography 60, 90, and 150 Angstrom
In contrast to methodologies • Silica activity is stabilized at
involving normal hydrophilic For the chromatographer who 7% water; pH (slurry) of 6.5-
silicas, many difficult separa- wants to try something a little 7.0 is standard
tions can be achieved using different at a reasonable cost, • TLC grade silicas are avail-
lipophilic silica layers and the GFSelect™ line of chro- able in 5-15 micron particle
reversed phase techniques. matographic silicas provides size distribution, 500 m2/gm
Given the continuing develop- several distinct features: specific surface area, 60
ment of HPLC methods, the Angstrom pore diameter
demand for TLC plates that • Automated processing pro- • Complementary products
can be used for orienting pre- vides tight 40-63 micron parti- are available, including TLC
plates, modified and reverse
CHROMATOGRAPHY
separations will also increase. cle size range where low fines
Such systems frequently use are preferred for higher reso- phase silicas, and spherical
C-18 and C-8 silanized silicas lution, and reproducibility silicas in 70, 100, 300, and 800
especially suited for the sepa- is critical Angstrom pore sizes
ration of very polar • Advanced production tech-
(hydrophilic) substrates nologies yield high
which previously could only GFS # Pore Particle Size Range
be separated by ion exchange 2797 40A 15-35 micron
chromatography. Macherey- 2798 40A 40-63 micron
Nagel has chosen to address 2799 40A 60-200 micron
this need only with C-18 2800 40A 200-500 micron
silanized 60 Angstrom (2-10 2801 60A 5-15 micron
micron) silicas that have been 2802 60A 15-35 micron
modified with different 2803 60A 40-63 micron
degrees of silanization. Please 2804 60A 60-120 micron
let us know if we can help you 2805 60A 120-200 micron
select or provide the product 2806 60A 60-200 micron
2807 60A 200-500 micron
that you need for your
reversed phase separation 2808 90A 15-35 micron
problem. 2809 90A 40-63 micron
2810 90A 60-200 micron
Macherey-Nagel offers a broad 2811 90A 200-500 micron
range of chromatography
2812 150A 40-63 micron
products designed to address 2813 150A 60-120 micron
the separations issues you are 2814 150A 60-200 micron
most likely to face in your work. 2815 150A 120-200 micron
Full information and valuable
technical data are available in 2816 60A 5-15 micron, TLC/Gypsum
the Macherey-Nagel 2817 60A 5-15 micron, TLC//Indicator
2818 60A 5-15 micron, TLC/Gyp.+ Ind.
Chromatography Products
guide.

10. The main section of the GFS
catalog provides specifica-
Specialty Silicas Grade 12 GFS #2780
28-200 mesh; may be particu-
tions and order information from W. R. Grace larly useful in hydrocarbon
for these products. and steroid separations.
Grace specialty silicas reflect
Other attractive features of decades of experience in sur- Grade 15 GFS #2783
this product line include face chemistry and related 30-60 mesh; narrower particle
• availability of bulk quanti- separations technology. Their size distribution provides
ties of most materials expertise in both adsorption enhancement of separations
• most grades can be modi- and absorption chemistries in achieved by Grade 12.
fied to provide standard, cus- major commercial markets
tom or reversed phase silicas includes industrial and spe-
Grade 22 GFS #2784
• ongoing development of cialty catalyst formulations.
60-200 mesh; complements
adsorbents for specialty appli- The following products find
CHROMATOGRAPHY
Grades 12 and 15.
cations such as ion chelation favor in a wide variety of
applications, and can be sup-
plied in bulk quantities in a Grade 59 GFS #2788
8 mesh, intermediate density
timely manner.
(25 lb/cu.ft.), low surface area
(300 m2/gm); a wide-pore,
non-decrepitating buffer gel
capable of high liquid adsorp-
tion in applications such as
color body removal; 99.6%
SiO2.
Grade 62 GFS #2789
60-200 mesh, 150 Angstrom;
intermediate density, suitable
for some catalyst support
applications.
Grade 922 GFS #2793
-200 mesh; general chromatog-
raphy use.
Grade 923 GFS #82
100-200 mesh, high purity
chromatographic silica suit-
able for ASTM D-1319-70.

11. Alumina, Brockmann I activity. Acidic
and neutral grades are pro-
Chromatographic duced by the measured addi-
Grades from Camag tion of HCl (gas) to basic alu-
mina, which is the natural
product of calcination of alu-
Chromatographic grade alumi- minum hydroxide.
na is a white, fine-grained
powder characterized by par-
ticle size, surface area, porosi-
ty and Brockmann activity.
Minimal moisture content
allows a designation of
Brockmann I activity for the
CHROMATOGRAPHY
most popular grades of alumi-
na. Less active grades
(Brockmann II-V) can be
achieved by adding
controlled amounts of mois-
ture to the material. All alumi-
na can absorb moisture from
the air, so care must be taken
in storage and handling in
order to preserve the
Grade Designation GFS # 2619 GFS # 2620 GFS # 2618
Camag Product Code 5016-A-I basic 507-C-I neutral 504-C-I acidic
pH of a 5% aqueous
suspension (stirred) 9.5 +/- 0.5 7.0 +/- 0.5 4.5 +/- 0.5
Specific surface (m2/gm) 150 +/- 10% 150 +/- 10% 150 +/- 10%
Brockmann activity I I I
Mean pore diam. (Angstrom) 60 60 60
Pore volume (mL/gm) ca. 0.25 ca. 0.25 ca. 0.25
Chloride (mval/gm) nil 0.03 0.14
Fe2O3 (%) 0.01 – 0.03 0.01 – 0.03 0.01 – 0.03
Na2O (%) 0.25 – 0.4 0.25 – 0.4 0.25 – 0.4
Bulk density (gm/L) ca. 920 ca. 920 ca. 920
Granulometry
>200 micron 10-20%; 160-200 micron 10-20%; 100-160 micron 35-80%;
71-100 micron 6-15%; 40-71 micron 2-10%; <40 micron 2-5%

12. Silica Gel Desiccants, this area; please inquire if you
Adsorbents, Molecular Sieves would need information on
more specialized silicas or
from W. R. Grace
zeolites that may be under
development.
The broad range of conditions
under which moisture adsorp-
These products all have 0.43
tion plays a critical step in
cc/gm pore volume, loss on
commercial and laboratory
ignition (1750°F) of ca. 6.5%,
processes has led to the
and bulk density of ca. 45
development of several types
lb/cu.ft. Typical equilibrium
of specialized products in the
adsorption at 10% relative
silica gel and molecular sieve
humidity is around 7 wt.%.
families. The products listed
CHROMATOGRAPHY
below address basic needs in
Feature Grade 03 Grade 13 Grade 40 Grade 41 Grade 47 Grade 48
(GFS #) 2779 2782 2785 1019 2786 2787
mesh on 8 30-200 6-14 3-9 1-2 mm 2-5 mm
indicator none blue ind. none none blue ind. blue ind.
use or drying need for fine MIL-D-3716B MIL-D-3716 99% SiO2 99% SiO2
criteria of gases particle size type II gr. H type I gr. H 750 m2/g 750 m2/g
(internal surface area)
Molecular Sieves 50% of the total volume of the
solid. Drying of common liq-
Grace Davison molecular uids and gases are typically
sieves are convenient bead performed using 3, 4, or 5
forms of specific aluminosili- Angstrom pore diameter zeo-
cates also known as zeolites. lites, chosen by virtue of the
Adjusting the Group I or molecular dimension of the
Group II component of the chemical to be adsorbed.
zeolite formulation allows the Moisture removal is frequent-
synthesis of material charac- ly carried out using 4A
terized by cavities having spe- (4 Angstrom) molecular
cific pore diameters. Internal sieves. The following GFS
channels comprise close to products are available:
TYPE FORM/SIZE GFS# FORM/SIZE GFS# FORM/SIZE GFS#
3A bead/4-8 mesh 2709 bead/8-12 mesh 963 powder/3-5 micron 2710
4A bead/4-8 mesh 2711 bead/8-12 mesh 964 powder/<5 micron 2712
5A bead/4-8 mesh 2713 bead/8-12 mesh 965 powder/3-5 micron 2714
13X bead/4-8 mesh 2715 bead/8-12 mesh 1067 powder/3-5 micron 2716
Please inquire regarding additional forms and types of zeolite/molecular sieve materials.

13. Florisil® from U.S. Silica
DOWEX® - Fine Mesh
The Florisil®line has found Spherical Ion Exchange Resins
extensive use in preparative
from Dow Chemical
and analytical chromatogra-
phy:
Dowex® resins have a long
and successful history in
• Activated magnesium sili-
areas such as fine chemical
cate (“magnesium-silica
and pharmaceutical column
gel”), ca. 15.5% MgO, 84%
separations. Based on a
SiO2
microporous copolymer of
• White, hard powdered mate- styrene and divinylbenzene
rial which is a highly selec- (DVB), these beaded materi-
CHROMATOGRAPHY
tive adsorbent als are designed for maximum
• TLC Grade, ultrafine (-325 resistance to oxidation, reduc-
mesh) powder suitable for tion, mechanical wear and
thin layer chromatography fracturing. Selectivity, consis-
• Reagent Grade, activated at tency and reliability in column
675o C, suitable for pesticide separations makes Dowex®
residue analysis resins the media of choice for
• Standard Grade, activated at a broad range of applications.
650oC, general chromato-
graphic adsorbent use The Dow resin nomenclature
reflects the type of resin and
Standard mesh cuts include degree of cross-linking to DVB
16-30, 30-60, 60-100, 60-200, that is present:
100-200, and -200. • Type I is a strong base (Cl-
form) anion exchange resin;
for example, a 1X2-100 resin
contains 2% DVB, 50-100
mesh size.
• 50W designates a strong
acid (H+ form) cation
exchange resin; Dowex
50WX4-200 has 4% DVB
cross-linking and a particle
size range of 100-200 mesh.
If you don’t find the DOWEX®
resin you need in the alpha-
betical section of this catalog,
please inquire.

14. GFS # Description Features
2642 Amberlite IR-120(Plus)
®
hydrogen form, strongly acidic,
gel-type, 8% cross-linked; catalyst
2643 Amberlite® IR-120, Na sodium form, gel-type, sulfonic
acid functionality, some catalyst use
2644 Amberlite® IRA-67 tertiary amine functionality, weakly basic,
1.6 meq/ml exch. capacity
2645 Amberlite® IRA-400(Cl) quat-ammonium functionality,
strongly basic, gel-type, chloride form
2646 Amberlite® IRA-410 strongly basic gel-type resin also
useful in catalytic applications
2647 Amberlite® IRA-900 strongly basic, macroreticular resin,
moderately high porosity
2648 Amberlite® IRC-50 weakly acidic resin, selectively
adsorbs alkaloids, amino acids
CHROMATOGRAPHY
2649 Amberlite® XAD-4 high surface area (750 m.sq./gm) polystyrene
adsorbent, 20-60 mesh
2652 Amberlyst® 15 strongly acidic, macroreticular,
suitable for non-aqueous catalysis
2653 Amberlyst® 15 (wet) strongly acidic, macroreticular,
suitable for aqueous catalysis
2654 Amberlyst® A-21 weakly basic (free base),
macroreticular, non-aqueous use, AMBERLITE®/AMBERLYST®
20-60 mesh
Resins and Polymeric
Adsorbents from
Rohm and Haas
Polymeric materials technolo-
gy at Rohm and Haas fostered
the development of a variety
of adsorbent media that are of
particular interest to synthet-
ic organic chemists and tech-
nicians dealing with separa-
tions and purifications in both
aqueous and non-aqueous
systems. Key characteristics
are provided above.
Please advise if you would like
to see additional Rohm and
Haas products offered in the
GFS catalog. Our objective is
to make GFS a more valuable
resource for you.

15. Drierite® Calcium Sulfate non-channeling, chemically Diatomaceous Earth
Desiccants from inert except toward water, Filter Aids
constant in volume, neutral
W. A. Hammond Co. Ltd.
in pH. Products having trade names
Drierite® is an all-purpose dry- such as Celite® and Celatom®
For further information on are recognized for their deri-
ing agent that is compatible
desiccant efficiencies see the vation from naturally occur-
with most chemical environ-
series of articles by David R. ring silicate materials deposit-
ments. It removes moisture
Burfield et al., starting with J. ed over millennia via the
efficiently from gases (includ-
Org. Chem. 1977, 42, 3060-5. decomposition of aquatic
ing air), refrigerants, organic
liquids and solids. Tests have plants related to algae
shown that the moisture Activated Carbon (diatoms). Their intricate
remaining in gases dried with micro geometric forms, poros-
CHROMATOGRAPHY
Activated carbons are typical- ity and inertness give them
Drierite® at ambient condi-
ly amorphous powders or great versatility in chemical
tions is 0.005 mg/liter. Air can
granules that are character- absorption and filtration
be dried to a dew point of
ized by high adsorptivity for processes. GFS has used them
-100°F, and organic liquids are
many gases, vapors, colloidal for decades; we now offer
often dried to the low part per
solids, organic substrates and them in this catalog for your
million range. Drying efficien-
some inorganic species. convenience.
cy is usually maintained even
Common uses include decol-
as application temperatures
orization and purification or
approach 100°C. Indicating
separation of organic sub-
forms containing 3% cobalt
strates. GFS offers several
chloride are available. See the
popular grades; let us know if
alphabetical section for GFS
you can’t find the activated
listings of both forms in 4, 6,
carbon you need.
8, 10-20, and 20-40 mesh sizes.
In liquid drying, Drierite®
instantly absorbs 6.6 wt.%
water by chemical action. In GFS # Code / Description
drying gases, its water capaci- 2634 Darco 4-12 mesh granular, acid washed, lignite
ty is 10 to 14 weight percent, carbon, general purpose use
attributable to combined 2635 Darco 12-20 mesh granular, acid washed, lignite
chemical and capillary action carbon, general purpose
that is derived from a granule 2636 Darco 20-40 mesh granular, acid washed, lignite
carbon, general purpose
pore space volume of 38%.
717 Darco G-60, -100 mesh powder, high-purity, used
Drierite® typically can be to purify/upgrade organics
regenerated numerous times. 2634 Darco KB,-100 mesh wet powder (<30% H2O);
It is non-toxic, non-corrosive, 1500 m2/g s.a. (dry), 1.5 cc/g pore volume (dry)
2640 Norit® RO 0.8, pellets, for liquid-phase adsorption

16. Specialty Reagents The following products find
frequent use as reagents in
ed by other standard proto-
cols such as dinitrophenylhy-
and Media for Ion Pair Chromatography: drazone derivatization.
Chromatography GFS #1202 SILICIC ACIDS; WHITE
Tetrabutylammonium hydrox- QUARTZ SILICA SAND
ide, 1.0 M in water.
The immense scope of chro-
GFS #2262 matographic techniques and
QUATERNARY AMMONIUM
Tetrabutylammonium hydrox- tools is necessary to keep
COMPOUNDS for Ion-Pair
ide, 0.4 M in water. pace with the growth and
Chromatography
technical sophistication of
GFS #2517 current separations technolo-
The GFS line of Electrometric
Tetrabutylammonium hydro- gies. Materials as elegant as
CHROMATOGRAPHY
and Analytical Reagent Grade
gen sulfate, 98%, crystal. high purity silicic acid and as
(EARG) quaternary ammoni-
simple as silica sand are both
um compounds includes sev-
GFS #2433 important resources for the
eral products of particular use
Tetrabutylammonium dihy- problem-solving chromatogra-
in the field of Ion-Pair
drogen phosphate, 98+%, pher. GFS now offers these
Chromatography. Acquired
crystal. materials for your conven-
from Sachem, Inc. (Austin TX)
ience:
in 1999, these EARG quats are
KETONE DERIVATIZING
high-purity materials especial-
AGENTS GFS # 2794 Silicic acid, -100
ly suitable for applications
mesh, loss on ignition <16%,
that must conform to analyti-
An example of a superior for chromatography
cal protocols having stringent
ketone derivatizing agent
purity requirements.
manufactured by GFS is: GFS # 2795 Silicic acid, <20
Characterized by low levels of
2-diphenylacetyl-1,3-indane- micron, 99.9% for chromatog-
alkali metals, chloride and
dione-1-hydrazone raphy
ash, the EARG quats meet the
(GFS #2366).
criteria for use in various
GFS # 2796 Silica sand, white
chromatographic protocols.
This reagent facilitates the quartz, -50+70 mesh, suitable
determination of volatile alde- for chromatography
hydes and ketones by HPLC,
even demonstrating great sen-
sitivities at very low concen-
trations. Using spectroscopic
or fluorometric techniques,
detection limits can be
enhanced to 0.25 parts per bil-
lion, surpassing limits provid-

17. GFS Products Better Buffers™
The GFS bio-analytical line
toward metal ion complexa-
tion. This is believed to be a
for also includes a line of “Better primary source of interfer-
Life Sciences Buffers”– Good’s buffer alter-
natives that are better suited
ences in protein analysis,
where the complexation of
to bioanalytical buffering situ- Cu(II) has been shown to be a
ations that are sensitive to particular problem. The
interferences caused by metal Better Buffers also allow the
ion complexations, especially minimization of amine-metal
GFS Bio-RefinedTM Products
copper ion. These non-com- complexation promoted by
We introduced our Bio-
plexing tertiary amine buffers the presence of donor atoms
Refined™ product line to meet
cover the entire pH range (3- such as hydroxymethyl and
the growing need in the Life
10) for which bioanalytical hydroxyethyl groups.
Sciences and biochemical
buffering is usually required.
CHROMATOGRAPHY
fields for high-purity, well-
This includes most of the cur- This GFS line also includes
characterized inorganic and
rent focus on pH-dependent three buffers studied by Good
organic salts for research and
phenomena observed in bio- and co-workers: MES, MOPS,
bio-analytical applications.
logical and physiological and PIPES; the newly avail-
These products are carefully
studies. able MOBS (4-N-morpholino)-
purified and checked to meet
butanesulfonic acid, is also
specific pH requirements as
The N-substituents on the shown on the chart of pH
well as for conformance to
Better Buffers render the ranges of non-complexing
optical absorbance and trace
amine sterically inaccessible buffers.
metal ion specifications.
In response to the need for DESPEN
greater assurances of bio- DEPP
chemical purity in many prod-
PIPBS
ucts, GFS is developing proto-
PIPPS
cols for qualifying products
for criteria such as the MOBS
absence of DNase, RNase and MOPS
protease. Detection method- PIPES
ologies such as advanced fluo-
TEEN
rescence spectrophotometry
will be used to ensure product MES
conformance to these specifi- DESPEN
cations. DEPP
PIPBS
PIPPS
PIPES
2 4 6 8 10 12

18. Since the catalyst is not on lize molecules with several
The Royer ® carbon, its contamination active sites, such as
Palladium Catalyst- effects are significantly less nucleotides or proteins.
A Breakthrough in than palladium on carbon.
The Royer Palladium Catalyst A short time later, Royer and
Palladium Catalysts is available in either powder colleagues2 demonstrated the
form or beads. We also have feasibility of using formic acid
manufactured and made avail- as a hydrogen donor for the
By employing a unique, able Platinum, Rhodium and removal of several protecting
patented Polyethylenimine- Ruthenium on the patented groups in the synthesis of
Polymer matrix to support Royer Substrate. peptides by catalytic transfer
palladium metal, we have cre- hydrogenation on palladium.
ated a new, highly effective The Royer® Products This idea was later improved
upon when it was discovered
CHROMATOGRAPHY
palladium catalyst which is
superior for hydrogenation While pursuing better meth- that the PEI “ghosts” would
reactions. ods of peptide synthesis, complex palladium as well as
Professor Gar Royer discov- other metals. Palladium was
The Royer Palladium ered a method of preparing a complexed on the PEI that
Catalyst’s high surface area new polymeric material which was on silica beads and then
makes it extremely easy to could be used for numerous reduced to the metal3. The
work with and eliminates the things. It was first reported in comparison of the rates of
need to bother with the the literature in 19771 and a hydrogenolysis of Cbz-alanine
messy and hard-to-handle patent application was filed. using formic acid at room
palladium on carbon. The patent was granted in temperature and pressure are
1982, US 4,326,009. shown in Fig.1. This catalyst
This Unique Palladium is available now as 1%
Catalyst Offers These The first published applica- Palladium on PEI/SiO2 beads
Advantages: tion of the material was as (GFS #1004) or powder (GFS
catalyst in the hydrolysis of #1005) and 3% Palladium on
1. Reusable p-nitrotrifluoroacetanilide and PEI/SiO2 powder (GFS #1068).
Unlike other palladium cat- involved the preparation of a These palladium catalysts can
alysts, the Royer Palladium polyethylenimine (PEI) also be used effectively with
Catalyst can be reused up “ghost”. PEI was deposited on hydrogen gas for reduction
to 10 times with little or no the surface of an alumina medium by decantation (in
activity loss. bead, cross-linked with glu- contrast with the filtration
2. Extremely low pyrophoric taraldehyde and the bead was required for palladium on car-
potential then dissolved away, leaving bon) and have low pyrophoric
3. Excellent stability the porous polymer shell or potential. That they are readi-
4. Easy separation from “ghost”. The cross-linked ly reusable is shown in Fig. 2.
reaction product polymer has many active sites
5. Little or no contamination and will complex or immobi-

19. Further work with the affinity Figure 1. Rate of Pd-catalyzed
of PEI polymers for anions led hydrogenolysis of Cbz-alanine
to the application by Royer using formic acid as the hydro-
and co-workers4 of several gen donor. Pd-PEI “ghosts”, 100
mg (•); Aldrich 10% Pd on char-
compositions of PEI polymers coal, lot no. 031597, 10 mg
to the separation of ( ); Research Inorganic/Organic
nucleotide mixtures by col- Chemical 10% Pd on charcoal,
umn chromatography. Several two different samples, 10 mg (X
and O); Pd-black, 1 mg (∆).
of these are shown in Figures
3, 4, and 5. The ion-exchange
resins are available now as
Royer® Anion Exchange
Resins; PEI 40 and 100 are
glutaraldehyde cross-linked
CHROMATOGRAPHY
material on silica beads 20-40
mesh and silica gel 40-200
mesh, GFS #1000 and #1001.
BZL-PEI 40 and 100 are benzy-
lated derivatives of the glu-
taraldehyde cross-linked Figure 2. Reuse of Royer
product in the same mesh Palladium Catalyst.
sizes, GFS #1002 and #1003, Atmospheric pressure
hydrogenation of 0.1 mole
respectively. PEI-X-40 and 100 nitrobenzene in 90 ml of
are the Xama cross-linked methanol with 2 g of 1%
materials, GFS #1065 and Royer Palladium Catalyst.
#1066.
1. Meyers, W.E.; Royer, G.P.; J. Am.
Chem. Soc., 99, 6141 (1977)
2. El Amin, B.; Anantharamaiah,
G.M.; Royer, G.P.; Means, G.E.; J.
Org. Chem., 44, 3442 (1979)
3. Coleman, D.R.; Royer, G.P.; J. Org.
Chem., 45, 2268 (1980)
4. Watanabe, K; Chow W.-S.; Royer,
G.P.;Anal. Biochem., 127, 155 (1982)

20. Figure 3. PEI-glutaraldehyde-sili-
ca resolution of adenosine, AMP,
ADP, and ATP. Conditions: column
size 28 X 0.6 cm; flow rate, 1
ml/min; one fraction equals 3 ml;
adenosine (0.8 µmol) eluted with
Tris-HCl (pH 8, 10 mM), AMP (2.4
µmol) with buffer containing 0.15
M NaCl, ADP (2.3 µmol) and ATP
(1.5 µmol) with a linear gradient
(0.3-0.5 M).
CHROMATOGRAPHY
Figure 4. Separation of adeno-
sine, AMP, ADP, and ATP on
PEI-Xama silica gel. The col-
umn (6 X 0.5 cm) was equili-
brated with Tris-HCl (10 mM,
pH 8); the nucleotides and
adenosine (1 mg each) were
applied in 2 ml of the same
buffer. Recoveries were quan-
titative: flow rate, 1 ml/min; one
fraction equals 3 ml.
Figure 5. (A) Resolution of a
mixture of 3’:5’-cyclic AMP (0.13
µmol) and MAP, ADP, ATP (1.3
µmol each). Conditions: PEI-
Xama silica gel column (6 X 0.5
cm); flow rate, 1 ml/min; fraction
volume equals 3 ml. Tris-HCl (pH
8, 10mM) was used to elute
3’:5”-cyclic AMP. The NaCl gra-
dient followed immediately.
(B) In this case, the Tris-HCl
was run for 30 min. Otherwise,
conditions are identical to those
of A.

21. Prices and Terms GFS Chemicals
Terms of Service
Limitation on GFS
Product Use, Warranties
and Liability
Terms of sale are net 30 days Unless otherwise specifically
GFS Warranty
with established credit, FOB certified in writing by GFS
GFS products are warranted
our plant in Columbus, Ohio. Chemicals, Inc. (GFS), GFS
to conform, on the date of
Unless otherwise instructed, products are not intended,
shipment, to the GFS specifi-
freight charges will be either approved or authorized for
cations in effect at the time of
prepaid and invoiced to the human or animal consump-
product manufacture. The
customer or COD at the dis- tion nor for use in any food,
GFS warranty obligation is
cretion of the Company. We drug or cosmetic applications.
limited to either replacement
reserve the right to make GFS products are warranted
of non-conforming product or
price changes without formal to meet our specifications as
the refund of the purchase
notice. GFS is proud to offer set forth in the GFS Lot
price. GFS products are not
the following credit card Analysis or other Company lit-
certified for or intended for
options for your convenience: erature specifically relating
direct use in food, drug, or
Visa, Mastercard, American thereto only. By accepting
cosmetic applications. Any
Express. GFS's products, Customer
other warranties, express or
Conditions
agrees to the limitations set
implied, that assume mer-
forth herein, assumes all
chantability or fitness for a
GFS products are sold for responsibility for the proper
particular purpose are dis-
manufacturing or laboratory use of such products in accor-
claimed. Under no circum-
use only and are not intended dance with these limitations,
stances shall GFS Chemicals,
or certified for direct use in and agrees to indemnify,
Inc. be liable for indirect, sec-
food, drug or cosmetic appli- defend and hold harmless GFS
ondary, or consequential dam-
cations. GFS Chemicals from and against any and all
ages of any nature, including
assumes no responsibility claims, suits and/or liability in
without limitation loss of prof-
connected with the use of our the event such products are
its or business interruption.
products or the information used in a manner contrary to
printed in our publications. In these limitations. GFS HERE-
no case is the purchase of our BY DISCLAIMS ANY AND ALL
products to be taken as a WARRANTIES, LIABILITY
license to violate or infringe AND/OR RESPONSIBILITY IN
upon any patent. CONNECTION WITH ANY USE
OR APPLICATION OF ITS
PRODUCTS IN A MANNER
CONTRARY TO THE LIMITA-
TIONS SET FORTH HEREIN.

22. ANY USE OR APPLICATION OF International Orders Transportation and Shipping
GFS PRODUCTS IN A MANNER Unless otherwise agreed to in For your information and ease
CONTRARY TO THESE LIMI- writing by GFS, all internation- of ordering, we have included
TATIONS SHALL BE AT THE al orders are shipped FOB DOT hazard information on
USER'S SOLE RISK, AND Columbus, Ohio USA - GFS our entire line of chemicals.
SHALL RELIEVE GFS OF ALL plant basis INCOTERMS 2000. All products in this catalog
WARRANTIES, LIABILITY AND Payment terms are set upon may be shipped by commer-
RESPONSIBILITY WITH acceptance of order. Letter of cial freight. Limitations on
RESPECT THERETO. Credit method is acceptable package size and quantity
These limitations may not be for orders in the amount of may be restricted by the carri-
modified, affected or super- $5,000 and above with GFS er and hazard fees may apply.
seded by Customer, nor by instructions. The freight for- Some products (Hazard Class
any language, term or provi- warder and bank utilized for 6.1) can be shipped by some
sion contained in Customer's LC shipments will be that of carriers only when contained
purchase order, confirmation GFS Chemicals. All prices in a special poison pack.
or other correspondence or shown are in U.S. dollars. There will be an extra charge
communication. Payment in U.S. funds, by wire for this service.
Return Goods
transfer or check drawn on a
Full Warranty and U.S. bank.
Safety: Material Safety
Limitations Statement at No return shipments will be
Data Sheets
www.gfschemicals.com/ accepted without our prior
Terms.asp permission and instructions
TSCA Compliance
We offer MSDS information on being obtained. If an error is
all of the products that we made in placing or filling your
GFS makes a concerted effort sell. An MSDS accompanies all order, please advise us
to foster customer awareness shipments when leaving our promptly so that we may take
of the limitations placed, by facility. If for any reason you proper action. No return
regulation, on the use of should need a second copy, goods or claims will be
research chemicals. GFS prod- we will be happy to fax one to allowed beyond 60 days.
ucts that are not listed on the you or you can retrieve one Orders for items not listed in
Toxic Substances Control Act from our website at this catalog or that have spec-
(TSCA) are to be used only for www.GFSChemicals.com ifications not printed in this
R&D purposes, by qualified catalog are considered spe-
technicians capable of under- cial/custom orders and shall
standing and communicating be processed by GFS on take-
their respective hazards. or-pay terms. A 25% restock
charge may apply to non-
custom orders.

23. Ordering
Information
Customer Service Representatives and
Technical Support are available Monday
through Friday from 8:00 a.m. to 5:30 p.m.
Eastern Time. Orders may be placed by mail,
phone, fax, e-mail or on our web site. Every
order receives an order confirmation sent
via fax or e-mail that includes items ordered,
pricing, shipping method, and estimated
shipping date.
By Mail: GFS CHEMICALS, INC.
Sales & Administration
PO Box 245
Powell, OH 43065
By Phone: 800-858-9682 (U.S.
and Canada)
(740) 881-5501 (International)
By Fax: (740) 881-5989
By E-mail: sales@gfschemicals.com
On the Web: www.gfschemicals.com
www.gfsinternational.com
Opening an account
GFS Customer Service Representatives
will be glad to help you open an account.
For details call: 1-800-858-9682.
(740) 881-5501 (International)
Additional Terms and conditions on page 112
Notice: GFS products are not certified or
intended for direct use in food, drug, or cos-
metic applications; they are warranted to meet
our specifications as given in GFS Lot Analysis
or other Company literature. Determination of
material suitability and/or merchantability for
purposes beyond these specifications is the
responsibility of the end user. WE HONOR