This document outlines the activities and requirements for a course on biotechnology. It includes lectures, presentations, exams, lab activities and field trips. There will be three exams covering introduction to techniques, applications of biotechnology, and international laws and guidelines. Students will work in groups to present on developing a GMO and create an exhibit for BioWeek. The course will also include virtual laboratory activities covering DNA extraction, PCR, gel electrophoresis, and microarrays. The last meeting will discuss isolating genes from plants and animals as well as human cloning and stem cell research.

1. ACTIVITIES
DATE ACTIVITY
JULY 5 LAST PPT LECTURE
JULY 9 VIRUTAL LABORATORY ACTIVITY
JULY 12 FIRST EXAMINATION
JULY 16 FREE DAY: LIBRARY WORK ON HOW TO DEVELOP
YOUR GMO
JULY 19-23 GROUP PRESENTATION (PPT)
-What to Develop
-Why Develop
-How will you Develop
-Dangers or Issues to Expect
JULY 26 APPLICATION OF BIOTECHNOLOGY LECTURE
SERIES

2. REQUIREMENTS
• GMO DEVELOPMENT PRESENTATION
• BIOWEEK EXHIBIT
• FIELD TRIP PORTFOLIO
• Expectations Before the Trip
• Learnings During the Trip
• Advocacy: Spreading knowledge of
Biotechnology to Others

3. exams
• Examination 1: INTRODUCTION TO TECHNIQUES IN
BIOTECHNOLOGY
• Examination 2: APPLICATIONS OF BIOTECHNOLOGY
• Examination 3: LAWS AND INTERNATIONAL
GUIDELINES CONCERNING BIOTECHNOLOGY
• Take-home (online)

4. LAST MEETING:
HOW WERE YOUR GENES ISOLATED?
• Golden Rice
• BT Corn
• Edible Vaccine in Potato
• GMO Soya
• Recombinant Hepatitis B
vaccine
www.designingdigitally.com

5. GENE ISOLATION
www.koihealth.org
Source of Genes Primers will amplify target
Gene of Interest
Amplicon for
“CONSTRUCT”
www.invitrogen.com
www.sciencedirect.com

6. POLYMERASE CHAIN
REACTION
www.neb.com
www.teachersdomain.org

7. MAKING YOUR
CONSTRUCT
www.addgene.org

8. EXAMPLES OF
RESTRICTION ENZYMES

9. TRANSFORMATION &
SELECTION MARKERS
bio.davidson.edu passel.unl.edu

10. BACTERIAL CLONING:
THE PROCESS

11. CONFIRMATION OF
EXPRESSION
• HOW WILL YOU KNOW IF THE GENE THAT
YOU INSERTED IS EXPRESSED?
viswagenbiotech.com en.wikipedia.org igece.org

12. www.designingdigitally.com

13. HOW ABOUT PLANTS?
A plant part
Is cultured
Callus
grows
Shoots
develop Shoots are rooted;
plant grows to maturity
clonal-solutions.com.au

14. DELIVERING GENES
(Agrobacterium & The Gene Gun)
apps.rhs.org.uk users.comcen.com.au

15. ehow.com weirdasianews.com cbc.ca

16. Cloning overview
• Organismal cloning produces one
or more organisms genetically
identical to the parent that
donated the single cell
• may lead to production of stem
cells for research and other
applications
• CLONE: genetically identical
individuals produced from one
parent
toonrefugee.com

17. Cloning plants
bbc.co.uk
• A totipotent cell is one that can generate a complete new
organism
• Plant cloning is used extensively in agriculture

18. Cloning animals
• Nuclear Transfer (e.g. BRANDY (2004) the cow
and DOLLY (1997) the sheep)
• the nucleus of an unfertilized egg cell or zygote
is replaced with the nucleus of a differentiated
cell
• Embryo Splitting (production of high value
herds and livestock breeds)

19. Nuclear transfer
skinnerscience.com

20. Embryo splitting
csus.edu

21. HUMAN CLONING &
STEM CELLS
Should we or Should we NOT?

22. 2 kinds of cloning
ipscell.com

23. The ivf way to have
a baby

24. Stem cell therapy
stemcellsforhope.com

25. Stem cells
for therapy
home.honolulu.hawaii.edu

26. READING ASSIGNMENT: Success
stories and fail stories using
stem cell therapy
Be sure to Identify the points of success and points of failure
thinkprogress.org

27. NEXT MEETING:
the virtual
laboratory
PER GROUP:
1. Laptop
2. Internet Source

28. ACTIVITY
THE VIRTUAL LABORATORY
Main reference: http://learn.genetics.utah.edu

29. WE WILL NEED AN
I-NET CONNECTION
• http://learn.genetics.utah.edu/content/labs/
extraction/
• http://learn.genetics.utah.edu/content/labs/pcr/
• http://learn.genetics.utah.edu/content/labs/gel/
• http://learn.genetics.utah.edu/content/labs/
microarray/
• http://learn.genetics.utah.edu/content/tech/cloning/
clickandclone/

30. FOR DISCUSSION
• What is the significance of DNA isolation?
• When is a PCR useful?
• Why do bands appear in an electrophoresis?
• How can a microarray data reveal gene expression?
• When do you think cloning is not indicated?

31. NEXT MEETING: EXAM
exam number 1