1. Thiocapsa roseopersicina
Prepared by :
1.Nurul Afiqah bt Mohd Rosli
2. Nurul Afiqah bt Ariffin
3. Nur Atifah Izzati bt Ishak
4. Nur ‘ Ain Syafiqah bt Kamaruddin

2. Thiocapsa roseopersicina

3. What is it??
• Purple sulfur bacteria from the
family of chromatiaceae
• T.roseopersicina was discovered
by Sergei Winogradsky in the
1880s when he was studying
sulfur bacteria
• Gram negative bacteria
• Non-motile bacteria

4. • Either rose colored or milky white
• Rose colored formed by the optical activity of
the carotenoids(pigments of chloroplast)

5. Where it can be found??
• T. roseopersicina is mainly found in microbial
mats in hypersaline and marine environments
• T. roseopersicina has often been found in
anaerobic waste stabilization ponds that have
a lot of dissolved organic matter
• T.roseopersicina grown in the light under
anaerobic conditions and in the darkness
under aerobic conditions

6. Cell metabolism
• T. roseopersicina has two different types of
metabolism that it can use based on the
environment
• Its main metabolism is anaerobic
photosynthesis which uses reduced sulfur
compounds as electron donors. The secondary
metabolism is chemolithotrophy in dark
environments,
• When T. roseopersicina is using its
photosynthetic metabolism, it has a faster
growing rate.

7. • When T. roseopersicina is growing under
chemolithotropic conditions, it has to
compete with colorless sulfur bacteria.
• The bacterium reaches a stationary phase at 3-
4 days with the photosynthetic conditions
whereas it reaches a stationary phase at 7-12
days with the chemolithotrophic conditions.

8. Structure
• T. roseopersicina has spherical cells that can
form tetrads. The cells are 1-3µm in size.
• The cells do not have gas vesicles.
• T. roseopersicina can use
hydrogen, sulfide, thiosulfate, acetate, propion
ate, pyruvate, malate, succinate, fumarate, fruc
tose, or glycerol as substrates.
• It cannot use formate or glucose as substrates
though

9. Anoxygenic photosynthetic
bacteria??
• Anoxygenic photosynthesis is the phototrophic
process where light energy is captured and stored
as ATP, without the production of oxygen.
• This means water is not used as primary electron
donor.
• There are three groups of bacteria that undergo
anoxygenic photosynthesis: phototrophic green
bacteria, phototrophic purple bacteria, and
heliobacteria.

10. The environment
• T. roseopersicina has optimal growth in a
medium with 0.3 M sodium chloride
• This is a lower concentration than that of the
seawater that T. roseopersicina is found, but that
is common for marine/halotolerant organisms
to have different optimal conditions than that
of the environment they are isolated from
• This is a good survival technique as the
osmolarity of the marine water fluctuates from
various occurrences like rain or evaporation

11. The colour is due to purple sulphur bacteria which grow
when there’s an abundance of plant debris in the bottom of
the pond, producing a lot of hydrogen sulphide gas (the
smell of rotten eggs). Purple bacteria use hydrogen
sulphide instead of water to power their photosynthesis.

12. • Pink ponds may be the result of a perfectly natural build up
of plant debris or the result of pollution by rotting organic
matter or sewage.
• So being pink doesn’t automatically mean your pond is
polluted – but it does mean it has a lot of organic matter.

13. Its function
T. roseopersicina can fix atmospheric N2
• This process is usually accompanied by Hydrogen
production
• T. roseopersicina has three different Nickel Iron
hydrogenases(catalyzes Hydrogen generation and
Hydrogen oxidation)
• Hydrogen oxidation is important for fuel cells

14. How to culture
• It is anaerobes bacteria
• Anaerobes can inoculated in an
agar slant using an inoculating
needle
• Needle is able to put the
anaerobes deep inside the agar.
• The anaerob can grow in the
anaerobic environment
provided.
• The agar slant should then be
refrigerated after suitable
growth is achieved

15. Application to biotechnology
• T. roseopersicina is an important bacterium
for microbial detoxification
• It is one of the few bacteria that can
completely oxidize dimethyl sulfide (DMS)
to sulfate

16. Thank you
Everything created for
reason..(^_^)