An overview of bioengineering and synthetic biology; definition, history, key research findings, scientists, current initiatives and future outlook.

1. Bioengineering: making life from scratch Melanie Swan MS Futures Group +1-650-681-9482 [email_address] http://www.melanieswan.com Slides: http://slideshare.net/LaBlogga/slideshows NIH, Bethesda MD September 25, 2008 Must build it to understand it

2. Synthetic biology Novel biological system

3. The new biology: virtuous feedback loop Model and simulate Investigate and experiment Build

4. Bio-SPICE Biological Simulation Program for Intra- and Inter-Cellular Evaluation http://biospice.org Model and simulate

5. Registry of Standard Biological Parts Build http://partsregistry.org

6. Parts Registry: select measurement device http://partsregistry.org

7. Parts Registry: obtain part sequence DNA Synthesizer http://partsregistry.org

9. Repressilator schema “ Noisy” cells Michael Elowitz Stanislas Leibler Repressilator A synthetic oscillatory network of transcriptional regulators Michael B. Elowitz & Stanislas Leibler Nature. 2000 Jan 20;403(6767):335-38. http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html

10. Construction of a genetic toggle switch in Escherichia coli Gardner TS, Cantor CR, Collins JJ Nature. 2000 Jan 20;403(6767):339-42. http://www.ncbi.nlm.nih.gov/pubmed/10659857 Toggle switch induction threshold Toggle switch plasmid Gardner Cantor Collins Genetic toggle switch

13. Further applications of bioengineering Coli-roid microbial camera Landmine sensing plant: Arabidopsis thaliana Amorphous computing Cellular colonies Natural genetic engineering Ciliate

14. Summary Model and simulate Investigate and experiment Build

15. Thank you Melanie Swan MS Futures Group +1-650-681-9482 [email_address] http://www.melanieswan.com Slides: http://slideshare.net/LaBlogga/slideshows NIH, Bethesda MD September 25, 2008