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Staining and Collodionization
- 2. General Staining Procedure MOUNT Xylene(1) Xylene DEPARAFFINIZATION CLEARING Xylene(1I) Xylene Absolute Alcohol (I) Absolute Alcohol (I) Absolute Alcohol (II) Absolute Alcohol (II) SECTIONS TO DEHYDRATION ALCOHOL 90% Alcohol 90% Alcohol 70% Alcohol 70% Alcohol Water STAIN
- 3. Staining of Paraffin Sections
- 4. Staining of Paraffin Sections MOUNT Xylene(1) Xylene DEPARAFFINIZATION CLEARING Xylene(1I) Xylene Absolute Alcohol (I) Absolute Alcohol (I) Absolute Alcohol (II) Absolute Alcohol (II) SECTIONS TO DEHYDRATION ALCOHOL 90% Alcohol 90% Alcohol 70% Alcohol 70% Alcohol Water STAIN
- 5. Staining of Celloidin Sections
- 6. Staining of Celloidin Sections Cellulose Nitrate Soluble in alcohol, treatment avoided PRECAUTIONS ◦ Stains on skin ◦ Dirty or oily slide ◦ Faulty staining solution ◦ Too much adhesive
- 7. Collodionization of Sections Definition If paraffin ribbons ◦ Contain air bubbles ◦ Torn ◦ Inadequately infiltrated Purpose Procedure
Hinweis der Redaktion
- After section cutting, and mounting on the slide , drained , dried to ensure firm attachment of section to slide
- Xylene, a solvent, 2x at 1-2 mins eachXylene not miscible with alcohol (DECREASING CONC)alcohol then replaced with water ,except if use alcoholic stainsincreasing conc for dehydration because most mountants are miscible with alcoholsecond xylene for refractive index, may stay there for an indefinite period until section is mounted on slideFloat off :: dirty or greasy slides,wet
- RemediesTopical application of 0.5% acid alcohol, then rinse with tap waterAlbumin fixative too old, to avoid, adhesives should be prepared in not too great amounts, last 2-3 monthsSection does not stain, prevent by sufficient ripened Hematoxylin, Impurities, improper washing off of paraffin, fixative, decalcifying solutionSections not clear under the microscope, xylol used to dissolve