This study aimed to characterize the genetic diversity of the Passiflora genus in Colombia using molecular markers. The study:
1) Analyzed 213 Passiflora individuals representing 151 species and 15 subgenera using chloroplast and mitochondrial DNA markers. This identified 280 chloroplast and 372 mitochondrial haplotypes.
2) Chloroplast DNA provided better resolution of relationships than mitochondrial DNA, separating the Decaloba subgenus and revealing geographic structuring of some groups.
3) Results only partially agreed with morphology-based taxonomy, suggesting divergence between genomes and mixed modes of organelle transmission in the genus. The study helped elucidate the evolution and systematics of Passiflora but also highlighted remaining questions
INCLUSIVE EDUCATION PRACTICES FOR TEACHERS AND TRAINERS.pptx
Study of the genetic diversity of the genus Passiflora L. and its distribution in Colombia
1. Study of the genetic diversity of the genus Passiflora L. and its distribution in Colombia John Ocampo École Supérieure d’Agronomie de Montpellier Université de Montpellier II Ph.D. ocampo.john@gmail.com
8. General objective To study the genetic diversity of the genus Passiflora L. and its biogeographic distribution in Colombia.
9.
10. To characterize the morphologic and molecular diversity at inter- and intra-specific levels.
11.
12. 1. Passifloraceae family Taxonomy 18 genera ca. 650 species America Africa Asia Killip (1938) 22 subgenera GenusPassifloraL. ca. 573 species America Asia Feuillet & McDougal (2003) 4 subgenera Astrophea DecalobaDeidamioides Passiflora
32. Economic importance – International markets P. edulis f. flavicarpa Yellow maracuja - Maracuyá P. ligularis Sweet granadilla P. edulis f. edulis Purple maracuja - Gulupa
33. Economic importance – International markets P. tripartita var. mollissima Curuba de Castilla P. tarminiana Curuba India P. maliformis Cholupa
34. Economic importance – International markets P. quadrangularis Giant granadilla - Badea P. popenovii Granadilla de Quijos P. alata Maracuja doce
35. Study Components I- Diversity distribution in Colombia (GIS) 1.a. Biogeography and conservation 1.b. Potential distribution and adaptation II- Morphological diversity (descriptors) III- Genetic diversity (CAPS – DNAcp/mt)
36. I – Diversity Distribution in Colombia (SIG) 1a. Biogeography and conservation of Colombian Passifloraceae .
37. Reported species HERBARIA National PSO - HAU - COL - FAUC - CUVC - FMB VALLE - MEDEL - CHOCO – TOLI - CAUP AFP - UIS – CDMB - COAH – JAUM - HUQ SURCO Foreign P : Museum d’Histoire Naturelle – Paris – France MO : Missouri Botanical Garden – USA MA : Real Jardin Botánico – Madrid – Espagne K :Kew Garden – London - Grande-Bretagne NY : New York Botanical Garden– USA LITERATURE Killip (1938, 1960), Escobar (1988, 1989) etc. 3.375 georeferenced data
38. Maximum Diversity in the American Andean Region, especially in Colombia and Ecuador Diversity Centre Diversity distribution of the American Passion Fruits 3.375 georeferenced data 16 4 23.5º N 167 reported species (26 new species for the country) Tropic of Cancer 20 14 20 70 15 17 10 10 18 55 25 13 78 31 48 53 30 32 36 167 0º 90 127 73 48 Tropic of Capricorn 2 16 -23.5º S 19 3
39. Species spatial distribution High density in the central mountain region. Low density North-East and South of the Andes. Very marked low density in lowlands, especially in the Orinoquian and Amanzonian regions. DIVA-GIS software
42. Public order555 collections from 17 of 32 departments TOTAL : 3.930 OBSERVATIONS
43. Elevation Species distribution by Biogoegraphic region Caribbean (38) Diversity concentrated in the Andean Region Pacific (36) Andes (123) Orinoquia (19) Amazonia (44)
45. Passifloraceae distribution in Colombia (Maximum Distance vs. Circular Area) Species of continental distribution Species of regional distribution and common endemic species R2= 0.779 P. vitifolia P. foetida P. auriculata P. quadrangularis P. nitida P. maliformis P. mollis P. mixta Endemic species P. cumbalensis P. menispermifolia P. ligularis P. suberosa P.arborea P. oerstedii P. capsularis P. seemannii P. coriaceae P.misera P. biflora P. spinosa P..coccinea P. rubra P. antioquiensis P. micropetala P. serratodigitata P. involucrata D. parviflora 1000 400
49. Date of recordsUICN Categories EX:Extinct EW:Extinct in the wild CR:Critically endangered EN:Endangered VU:Vulnerable NT:Near threatened LC:Least concern DD:Deficient Data
50. 1. Diversity and potentiel distribution 1b. Diversity distribution and in situ conservation of the Colombians Passifloraceae.
51. Software DIVA-GIS Speciesrichness and diversity Herbarium data: 3.330 Literature data: 45 Collection data: 555 3.930 georeferenced data
52. 1 2 Nine ultra- diverse zones in the Andes, four sub-explored (1, 6, 8, 9) 3 4 Species richness 5 6 7 8 9 Species potential distribution “Hotspot” Software DIVA-GIS
74. e. Qualitative data dendrogram (nNeighbour Joining, 32 characters) n = 12 n = 6 n = 10 n = 9 n = 6 n = ?? Distances Sokal & Michener
75. Conclusions The new descriptors list has permitted to rank species together with taxonomy in a coherent way. However, there are some exceptions. The analysis is coherent with Killip's classification (1938) at the subgenus level. Descriptors related to the flower permit a higher discrimination between subgenera and species.
94. The data set must be interpreted taking into account the geological context of tropical flora separation and the formation of the Andean mountain range.