Powerpoint accompanying research paper on the Teratogenic effects of Fipronil (GABAergic antagonist) on the neurological development of zebrafish (as model of vertebrates).

1. The Effects of fipronil on notochord development in Zebrafish Joshua Mendoza-Elias Biology 205L Experiments in Developmental Biology Fall 2006

3. Background- Accordian Phenotype

5. Materials & Methods Control (0.1% acetone) 0.7  M Fipronil (0.1% acetone) 1.1  M Fipronil (0.1% acetone) 2.3  M Fipronil (0.1% acetone) Notochord Morphology Acridine Orange Staining Behavioral Response

7. 36 hrs - 0.7  M

8. 36 hrs - 1.1  M

9. 36 hrs - 2.3  M

11. 48 hrs - 0.7  M

12. 48 hrs - 1.1  M

13. 48 hrs - 2.3  M

15. 36 hrs, 48 hrs - 0.7  M

16. 36 hrs, 48 hrs - 1.1  M

17. 36 hrs, 48 hrs – 2.3  M

18. Results 39 Total moderate: Total severe: 15 Total WT: 7 Total moderate: Total severe: Total WT: 36 hrs 48 hrs 23 31 10 24.59% 69.93% 11.47% 35.93% 48.43% 15.62%

19. Results-Acridine Orange Staining

22. References: [1] Stehr. C., Linbo, L.L., Incardona, J. P, Scholz, N. L. 2006. The developmental neurotoxicity of fipronil: notochord degeneration and locomotor defects in zebrafish embryos and larvae. Toxicological Sciences . 2006 92 ( 1): 270 -278. [2] Incardona, J., Stehr, C., Linbo, T., Scholz, N. Fipronil causes locomotor defects in developing zebrafish. 2006. Marine Environmental Research. 2006. 62 : S74 -S75. [3] Cole, Loretta M., Russel A. Nicholson, and John E. Casida. 1993. Action of phenylpyrazole insecticides at the GABA-gated chloride channel. Pest. Biochemical Physiology . 46 : 47-54. [4] Hamon, Nicholas, Richard Shaw, and Henry Yang. 1996. Worldwide development of fipronil insecticide. Proc.-Beltwide Cotton Conference. 2 : 759-765. [5] Colliot, F., K.A. Kukowskim P.W. Hawkins, and D.A. Robers. 1992. Fipronil: a new soil and foliar broad spectrum insecticide. Brighton Crop Proteonomics. Conf.-Pests Dis. 1 :29-34.