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FOLEY’S CATHETER
• Foley’s catheter is a self-retaining
  catheter made up of latex.
• It is made to be self-retaining by means
  of a balloon which should be inflated
  with saline.
• In cases of intractable epistaxis or
  bleeding oesophageal varices the
  balloon is inflated with air.
INDICATIONS
• URINARY:
To monitor urine output in case of
  shock or renal failure.
To differentiate anuria from retention.
For urinary incontinence.
To give bladder washes (in Cystitis)
For suprapubic cystostomy.
• NON-URINARY:
To arrest post-nasal bleeding in cases
  of intractable epistaxis.
To arrest bleeding from oesophageal
  varices in case of portal hypertension.
• CONTRAINDICATIONS:
In the presence of urethral trauma

• COMPLICATIONS:
Injury to urethra or urinary bladder.
Inadvertent catheterization of the
  vagina may occur.
Urinary tract infection in the absence
  of aseptic precautions.
PROCEDURE
 Sedate the patient, if necessary
 Cleanse the urethral meatus and penis
  or the perineal area with povidone
  iodine solution.
 Select the Foley’s catheter of the
  appropriate size:
 8 Fr: newborns
 10 Fr: most children
 12 Fr: older children
 The catheter tip should be well
  lubricated.
 EQUIPMENT:
  Sterile gloves - consider Universal
  Precautions
  Sterile drapes
  Cleansing solution e.g. Savlon
  Cotton swabs
  Forceps
  Sterile water
  Foley catheter
  Syringe
  Lubricant (water based jelly or xylocaine jelly)
  Collection bag and tubing
 POSITION: Place the patient supine with
  knees flexed.
 MALE:
 Gently grasp and extend the penile shaft to
  straighten out the urethral pathway.
 Hold the catheter near the distal tip and
  advance it up the urethra unless resistance or
  an obstruction is encountered.
 If resistance is encountered, select a smaller
  catheter.
 The catheter should be passed into the
  bladder all the way to the Y-connection.
 Inflate the balloon after advancing the
  catheter all the way to the Y-connection.
 Tape the catheter to the child’s leg.
• FEMALE:
Carefully spread the labia.
A well-lubricated, pre-tested Foley
  catheter is introduced into the bladder.
Advance the catheter to its entire
  length before inflating the balloon.
After withdrawing the catheter, after a
  dunking sensation is
  appreciated, secure it with tape.
 TO REMOVE THE CATHETER:
Attach a syringe and aspirate the saline
balloon gets deflated and the catheter can be
removed easily.
If this does not succeed, the balloon can be
deflated by:
 Overinflate the balloon by injecting more
   saline till it bursts.
 Cut the side-channel distal to the outer
   balloon and go on cutting it upto the meatus
   till the balloon gets deflated.
 The balloon can be punctured via per-rectal
   route or suprapubic route.
URINARY DRAINAGE BAG
 It is a disposable plastic bag which is
  marked in millilitres, so as to measure the
  urine output.
 The bag is closed at the upper end and
  has a plastic tube opening into it.
 There is a valve present at the opening of
  the plastic bag into the drainage bag to
  prevent urine collected in the drainage
  tube to re-enter the tube.
 The plastic tube (inlet) has a connector
  at the other end which can be connected
  to a catheter.
• The bag also has an outlet at the upper
  end which is usually kept closed, but
  can be used to empty the contents of
  the drainage bag by inverting the bag.
• The urinary drainage bag is placed at a
  lower level than the body to facilitate
  urine drainage by gravity.
FLATUS TUBE
 It is made up of India rubber.
 It is thick and stout and has a bulbous
  rod with two eyes at the tip.

 INDICATIONS:
Diagnostic use:
Diagnosis of intestinal obstruction
Diagnosis of volvulus
Therapeutic use:
To remove gaseous obstruction
In typhoid lymphatics
In paralytic ileus
In volvulus
CONTRAINDICATIONS
• Recent rectal or prostatic surgery
• Diseases of the rectal mucosa
• Immunosuppressed patients
 Equipment:
a) Take a sterile flatus tube in a bowl.
b) Swabstick
c) Vaseline bottle
d) Draw mackintosh and drawsheet.
e) Two kidney trays
PROCEDURE:
1) Arrange them in such a manner that it will be
   comfortable for the patient and handy to work.
2) It will also save the time and energy.
3) Explain the procedure to the patient and relatives to gain
   their co-operation.
4) Provide privacy by putting curtain, so that the patient
   will not feel shy.
5) To prevent the blanket from getting spoil, fold it and keep
   it at the foot site.
6) Place the draw mackintosh and draw the sheet under his
   waist to prevent the bed from getting wet.
7) Give him left lateral position on the bed.
8) Loose the garments of the waist and expose only the
   necessary portion, so that the patient will not hesitate.
9) Lubricate the flatus tube at eye side, up to 3" to 4" with vaseline to
    prevent the friction, as the mucus membrane of the rectum is very
    delicate.
10) Touch the tip of flatus tube to the anus so that the sphincter muscles
    of the anus constrict and immediately relaxes, at that time insert it in
    anal canal gently but quickly, keeping the free end of the flatus tube
    under the lotion in kidney tray.
11) Insert the tube 7 to 10 inches and observe the bubbles and liquid
    stools in the lotion.
12) When the bubbles are stopped then move the tube little bit inside
    and outside, and observe for the bubbles. After that remove the tube
    and keep it in other kidney tray. See that the tube will not touch the
    floor.
13) If the bubbles are observed more then write the result 'good', if not
    then mark the result 'poor'.
14) Remove the draw sheet and mackintosh. Make the patient
    comfortable. Do the bed neat
15) Record the date, time and result of flatus tube on the case paper
    and inform the sister incharge.
TUBERCULIN SYRINGE

It is 1cc syringe with a plastic piston (plastic
  syringe), or a metal piston ( glass syringe)
USES :
• To administer PPD for Mantoux test.
• To administer BCG vaccine.
• To adminiter test doses of drugs such as
  penicillin.
• Provocative Testing – to test for allergens in
  Bronchial asthma , atopy.
• Insulin injections in Diabetes Mellitus.
• Giving small doses of drugs. Eg. Gentamicin,
  Phenobarbitone , Digoxin.
MANTOUX TEST
Tuberculin PPD 2 T.U./0.1 ml, solution for
injection:
1 dose = 0.1 ml contains 0.04 microgram
  Tuberculin PPD.
– Store at 2°C - 8°C, protected from light
PROCEDURE OF MANTOUX TEST
Diameter of induration    Interpretation               Action
Less than 6mm             Negative                     Previously unvaccinated
                                                       individuals may be given
                                                       BCG provided there are no
                                                       contraindications.
6mm or greater but less   Hypersensitive to tuberculin Should not be given BCG
than 15mm                 protein. May be due to
                          previous TB infection, BCG
                          or
                          exposure to atypical
                          mycobacteria


>= 15mm                   Strongly hypersensitive to   Should not be given BCG.
                          tuberculin protein           Refer for further
                          Suggestive of TB infection   investigation
                          or                           and supervision which may
                          disease                      include chemotherapy.
BCG Vaccination
• It induces primarily cell mediated immunity.
• Administered at or soon after birth
• Supplied in the form of lypophilised or freeze
  dried powder, in a vaccum sealed dark colored
  multidose vial.
  Reconstituted with normal saline.
• Extremely sensitive to light and heat. Thus,
  cold chain should be maintained.
• In lypophilised form, it remains potent for
  upto a year at 2-8 ˚C.
• Dose – 0.1ml
• Site- convex aspect of left shoulder at the insertion
  of deltoid to allow for easy identification of scar.
• Route of administration – intradermal
• Multiplication of BCG bacilli      papule at 2-3
  weeks      4-8 mm in size at 5-6 weeks
      ulceration     heals by scarring around 6-12
  weeks
• Adverse effects – persistent ulcer with delayed
  healing ipsilateral axillary or cervical
  lymphadenopathy, and rarely abscess and sinus
  formation.
• Positive reaction to tuberculin test 4-12 weeks
  after immunisation.
SCALP VEIN NEEDLE
• It consists of a metallic needle attached to a plastic tubing.
• At the junction of the needle and the tubing, there is butterfly
  shaped plastic holder which facilitates easy insertion of the
  scalp needle into the vein.
• The plastic holder is flexible and colour coded .eg . Black is no
  22.
• The commonly used needles are from no 22 to no 24.
• There is an inverse relation between the guage number and
  the internal diameter.
• Higher the guage number , small is the diameter of needle.
  Thus 24G needle is smaller in diameter than 22G needle.
USES
• Collection of blood
• Infusion of iv fluids, drugs, blood etc.
• ABG analysis
LUMBAR PUNCTURE
INDICATIONS :
• Diagnostic :                   • Therapeutic :
   – Infectious                     –   Analgesia
      • Meningitis
      • Encephalitis                –   Anesthesia
   – Inflammatory                   –   Antibiotics
      • Multiple Sclerosis
                                    –   Antineoplastics
      • Gullain-Barre syndrome
   – Oncologic
   – Metabolic
   – Spontaneous
     subarachnoid
     hemorrhage
CONTRAINDICATIONS :
• Increased intracranial pressure
   – Cerebral herniation
   – Impending herniation
   – Possible increased ICP and focal neuro signs
• Coagulopathy
• Prior lumbar surgery
• Severe vertebral osteoarthritis or degenerative
  disc disease
• Significant cardiorespiratory compromise
• Infection near the puncture site
• Space occupying lesion
EQUIPMENT :
      • Spinal needle
          – Less than 1 yr: 1.5in
          – 1yr to middle childhood: 2.5in
          – Older children and adults:
            3.5in
      •   Three-way stopcock
      •   Manometer
      •   4 specimen tubes
      •   Local anesthesia
      •   Drapes
      •   Betadine
PROCEDURE :
• Performed with the
  patient in the lateral
  recumbent position.
• A line connecting the
  posterior superior iliac
  crest will intersect the
  midline at approx. the
  L4 spinous process.
• Spinal needles entering
  the subarachnoid
  space at this point are
  well below the
  termination of the
  spinal cord.
• LP in older children may
  be performed from L2 to
  L3 interspace to the L5 to
  S1 interspace.
• At birth, the cord ends at
  the level of L3.
• LP in infant may be
  performed at the L4 to L5
  or L5 to S1 interspace.
• Position the patient:
  – Generally performed in
    the lateral decubitus
    position.
  – A pillow is placed under
    the head to keep it in the
    same plane as the spine.
  – Shoulders and hips are
    positioned. perpendicular
    with the table.
  – Lower back should be
    arched toward
    practitioner.
a. Ligament flavum is a strong,
   elastic, yellow membrane
   covering the interlaminar
   space between the
   vertebrae.
b. Interspinal ligaments join
   the inferior and superior
   borders of adjacent spinous
   processes.
c. Supraspinal ligament
   connects the spinous
   processes
• A topical anesthetic (e.g. EMLA cream) can be
  applied 30 to 60 minutes before performing the
  puncture to minimize pain on penetration.
• Either a sitting or lateral decubitus position can
  be used .
• Monitor the patient visually and with pulse
  oximetry for any signs of respiratory difficulty as
  a result of assumed position.
• The subarachnoid space must be entered below
  the level of spinal cord termination.
• The spine should be flexed maximally to
  increase spacing between spinous processes.
• The patient’s back should be carefully prepared and
  draped using provided disinfecting solution and
  drapes.
• Orient yourself anatomically and find the L4 spinous
  process at the level of iliac crests
• Palpate a suitable interspace distal to this level.
• Infiltrate 2% Lidocaine subcutaneously (without
  epinephrine to prevent cord infarction should it be
  introduced into the cord by accident) with a fine
  needle.
• A field block can be applied injecting into and on
  either side of the interspinous ligaments.
• Identify the two spinal processes in between which
  the needle will be introduced, penetrate the skin
  and slowly advance the tip of the needle at about
  10 degrees cephalad (i.e. toward the patient’s
  umbilicus).
• Remove the stylet and check for clear fluid will flow
  from the needle when the subarachnoid space has
  been penetrated.
• The ligaments offer resistance to the needle, and a
  “pop” is often felt as they are penetrated.
• Withdraw the needle leaving the tip in, recheck the
  landmarks and slowly progress the needle again.
• Measure the opening pressure using the manometer
  by attaching it via a stopcock to the spinal needle.
• Normal opening pressure ranges from 10 to 100 mm
  H2O in young children and 60 to 200 mm H2O after
  eight years of age
• CSF volume of 1cc obtained in 3 tubes.
• In the neonate, 2ml in total can be safely
  removed.
• In an older child 3 to 6 ml can be sampled
  depending on the child’s size.
• Tube 1 is used for determining protein and
  glucose
• Tube 2 is used for microbiologic and cytologic
  studies
• Tube 3 is for cell counts and serologic tests for
  syphilis
COMPLICATIONS :

•   Herniation
•   Cardiorespiratory compromise
•   Pain
•   Headache (36.5%)
•   Bleeding
•   Infection
•   Subarachnoid epidermal cyst
•   CSF leakage
BONE MARROW ASPIRATION
INDICATIONS :
• Diagnostic :
 - Idiopathic Thrombocytopenic Purpura
 - Aplastic Anemia
 - Leukemia
 - Megaloblastic Anemia
 - Infections e.g. Kala Azar
 - Storage disorders e.g. Gaucher’s disease
 - PUO
 - Myelofibrosis
• Therapeutic :
 - Bone Marrow Transplantation
CONTRAINDICATIONS :

• Hemorrhagic disorders such as congenital
  coagulation factor deficiencies (eg,
  hemophilia), disseminated intravascular
  coagulation and concomitant use of
  anticoagulants.
• Skin infection or recent radiation therapy at
  the sampling site.
• Bone disorders such as osteomyelitis or
  osteogenesis imperfecta.
PROCEDURE :
• Obtain consent from a parent or guardian.
• If the posterior iliac crest is the chosen site, patients
  are generally placed in the lateral decubitus position
  or the prone position
• Sterilize the site with the sterile solution
• Place a sterile drape over the site, and administer
  local anesthesia, letting it infiltrate the skin, soft
  tissues, and periosteum.
• After local anesthesia has taken effect, make an
  incision through which the bone marrow aspiration
  needle can be introduced .
• If a guard is present, should be removed before starting
  bone marrow aspiration, to ensure adequate depth of
  penetration..
• In general, the needle should be advanced at an angle
  completely perpendicular to the bony prominence of
  the iliac crest.
• Once the needle passes through the cortex and enters
  the marrow cavity, it should stay in place without being
  held.
• Once the periosteum has been penetrated, pressure is
  used to advance the needle through the cortex and
  rotate the needle in a semicircular motion, alternating
  clockwise and counterclockwise movements.
• If the patient is in the lateral position, the hip may
  be stabilized with the other hand to get a better
  feel for the position and depth of the needle.
• The thumb of this hand can be to mark the
  desired site and to prevent accidental
  repositioning of the needle.
• A slight give will be felt, after which you will feel
  that the needle is fixed solidly within the bone.
• Remove the stylet and aspirate approximately 1
  ml of unadulterated bone marrow into a syringe.
• Specimen is taken and is assessed for the
  presence of bony spicules.
• If the specimen shows spicules, the specimen
  should be used to make smear slides immediately.
• If spicules are sparse or are not present, a new
  sample should be obtained from a slightly
  different site.
• The needle is left in place and sequential syringes
  are filled that have been prepared with heparin or
  other anticoagulants or preservatives, depending
  on the requirements for specific studies to
  withdraw samples for additional analysis.
• Then remove the needle, either after reinserting
  the stylet or with the syringe attached.
COMPLICATIONS :

•   Hemorrhage
•   Infection
•   Persistent pain at the marrow site
•   Retroperitoneal hematomas
•   Trauma to neighboring structures (e.g.,
    lacerations of a branch of the gluteal artery)
    and soft tissues

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Paediatric procedures part 2

  • 1.
  • 3. • Foley’s catheter is a self-retaining catheter made up of latex. • It is made to be self-retaining by means of a balloon which should be inflated with saline. • In cases of intractable epistaxis or bleeding oesophageal varices the balloon is inflated with air.
  • 4. INDICATIONS • URINARY: To monitor urine output in case of shock or renal failure. To differentiate anuria from retention. For urinary incontinence. To give bladder washes (in Cystitis) For suprapubic cystostomy.
  • 5. • NON-URINARY: To arrest post-nasal bleeding in cases of intractable epistaxis. To arrest bleeding from oesophageal varices in case of portal hypertension.
  • 6. • CONTRAINDICATIONS: In the presence of urethral trauma • COMPLICATIONS: Injury to urethra or urinary bladder. Inadvertent catheterization of the vagina may occur. Urinary tract infection in the absence of aseptic precautions.
  • 7. PROCEDURE  Sedate the patient, if necessary  Cleanse the urethral meatus and penis or the perineal area with povidone iodine solution.  Select the Foley’s catheter of the appropriate size:  8 Fr: newborns  10 Fr: most children  12 Fr: older children  The catheter tip should be well lubricated.
  • 8.  EQUIPMENT: Sterile gloves - consider Universal Precautions Sterile drapes Cleansing solution e.g. Savlon Cotton swabs Forceps Sterile water Foley catheter Syringe Lubricant (water based jelly or xylocaine jelly) Collection bag and tubing  POSITION: Place the patient supine with knees flexed.
  • 9.  MALE:  Gently grasp and extend the penile shaft to straighten out the urethral pathway.  Hold the catheter near the distal tip and advance it up the urethra unless resistance or an obstruction is encountered.  If resistance is encountered, select a smaller catheter.  The catheter should be passed into the bladder all the way to the Y-connection.  Inflate the balloon after advancing the catheter all the way to the Y-connection.  Tape the catheter to the child’s leg.
  • 10. • FEMALE: Carefully spread the labia. A well-lubricated, pre-tested Foley catheter is introduced into the bladder. Advance the catheter to its entire length before inflating the balloon. After withdrawing the catheter, after a dunking sensation is appreciated, secure it with tape.
  • 11.  TO REMOVE THE CATHETER: Attach a syringe and aspirate the saline balloon gets deflated and the catheter can be removed easily. If this does not succeed, the balloon can be deflated by:  Overinflate the balloon by injecting more saline till it bursts.  Cut the side-channel distal to the outer balloon and go on cutting it upto the meatus till the balloon gets deflated.  The balloon can be punctured via per-rectal route or suprapubic route.
  • 13.  It is a disposable plastic bag which is marked in millilitres, so as to measure the urine output.  The bag is closed at the upper end and has a plastic tube opening into it.  There is a valve present at the opening of the plastic bag into the drainage bag to prevent urine collected in the drainage tube to re-enter the tube.  The plastic tube (inlet) has a connector at the other end which can be connected to a catheter.
  • 14. • The bag also has an outlet at the upper end which is usually kept closed, but can be used to empty the contents of the drainage bag by inverting the bag. • The urinary drainage bag is placed at a lower level than the body to facilitate urine drainage by gravity.
  • 16.  It is made up of India rubber.  It is thick and stout and has a bulbous rod with two eyes at the tip.  INDICATIONS: Diagnostic use: Diagnosis of intestinal obstruction Diagnosis of volvulus
  • 17. Therapeutic use: To remove gaseous obstruction In typhoid lymphatics In paralytic ileus In volvulus
  • 18. CONTRAINDICATIONS • Recent rectal or prostatic surgery • Diseases of the rectal mucosa • Immunosuppressed patients
  • 19.  Equipment: a) Take a sterile flatus tube in a bowl. b) Swabstick c) Vaseline bottle d) Draw mackintosh and drawsheet. e) Two kidney trays
  • 20. PROCEDURE: 1) Arrange them in such a manner that it will be comfortable for the patient and handy to work. 2) It will also save the time and energy. 3) Explain the procedure to the patient and relatives to gain their co-operation. 4) Provide privacy by putting curtain, so that the patient will not feel shy. 5) To prevent the blanket from getting spoil, fold it and keep it at the foot site. 6) Place the draw mackintosh and draw the sheet under his waist to prevent the bed from getting wet. 7) Give him left lateral position on the bed. 8) Loose the garments of the waist and expose only the necessary portion, so that the patient will not hesitate.
  • 21. 9) Lubricate the flatus tube at eye side, up to 3" to 4" with vaseline to prevent the friction, as the mucus membrane of the rectum is very delicate. 10) Touch the tip of flatus tube to the anus so that the sphincter muscles of the anus constrict and immediately relaxes, at that time insert it in anal canal gently but quickly, keeping the free end of the flatus tube under the lotion in kidney tray. 11) Insert the tube 7 to 10 inches and observe the bubbles and liquid stools in the lotion. 12) When the bubbles are stopped then move the tube little bit inside and outside, and observe for the bubbles. After that remove the tube and keep it in other kidney tray. See that the tube will not touch the floor. 13) If the bubbles are observed more then write the result 'good', if not then mark the result 'poor'. 14) Remove the draw sheet and mackintosh. Make the patient comfortable. Do the bed neat 15) Record the date, time and result of flatus tube on the case paper and inform the sister incharge.
  • 22. TUBERCULIN SYRINGE It is 1cc syringe with a plastic piston (plastic syringe), or a metal piston ( glass syringe)
  • 23.
  • 24. USES : • To administer PPD for Mantoux test. • To administer BCG vaccine. • To adminiter test doses of drugs such as penicillin. • Provocative Testing – to test for allergens in Bronchial asthma , atopy. • Insulin injections in Diabetes Mellitus. • Giving small doses of drugs. Eg. Gentamicin, Phenobarbitone , Digoxin.
  • 25. MANTOUX TEST Tuberculin PPD 2 T.U./0.1 ml, solution for injection: 1 dose = 0.1 ml contains 0.04 microgram Tuberculin PPD. – Store at 2°C - 8°C, protected from light
  • 27. Diameter of induration Interpretation Action Less than 6mm Negative Previously unvaccinated individuals may be given BCG provided there are no contraindications. 6mm or greater but less Hypersensitive to tuberculin Should not be given BCG than 15mm protein. May be due to previous TB infection, BCG or exposure to atypical mycobacteria >= 15mm Strongly hypersensitive to Should not be given BCG. tuberculin protein Refer for further Suggestive of TB infection investigation or and supervision which may disease include chemotherapy.
  • 28. BCG Vaccination • It induces primarily cell mediated immunity. • Administered at or soon after birth • Supplied in the form of lypophilised or freeze dried powder, in a vaccum sealed dark colored multidose vial. Reconstituted with normal saline. • Extremely sensitive to light and heat. Thus, cold chain should be maintained. • In lypophilised form, it remains potent for upto a year at 2-8 ˚C.
  • 29. • Dose – 0.1ml • Site- convex aspect of left shoulder at the insertion of deltoid to allow for easy identification of scar. • Route of administration – intradermal • Multiplication of BCG bacilli papule at 2-3 weeks 4-8 mm in size at 5-6 weeks ulceration heals by scarring around 6-12 weeks • Adverse effects – persistent ulcer with delayed healing ipsilateral axillary or cervical lymphadenopathy, and rarely abscess and sinus formation. • Positive reaction to tuberculin test 4-12 weeks after immunisation.
  • 30. SCALP VEIN NEEDLE • It consists of a metallic needle attached to a plastic tubing. • At the junction of the needle and the tubing, there is butterfly shaped plastic holder which facilitates easy insertion of the scalp needle into the vein. • The plastic holder is flexible and colour coded .eg . Black is no 22. • The commonly used needles are from no 22 to no 24. • There is an inverse relation between the guage number and the internal diameter. • Higher the guage number , small is the diameter of needle. Thus 24G needle is smaller in diameter than 22G needle.
  • 31.
  • 32. USES • Collection of blood • Infusion of iv fluids, drugs, blood etc. • ABG analysis
  • 34. INDICATIONS : • Diagnostic : • Therapeutic : – Infectious – Analgesia • Meningitis • Encephalitis – Anesthesia – Inflammatory – Antibiotics • Multiple Sclerosis – Antineoplastics • Gullain-Barre syndrome – Oncologic – Metabolic – Spontaneous subarachnoid hemorrhage
  • 35. CONTRAINDICATIONS : • Increased intracranial pressure – Cerebral herniation – Impending herniation – Possible increased ICP and focal neuro signs • Coagulopathy • Prior lumbar surgery • Severe vertebral osteoarthritis or degenerative disc disease • Significant cardiorespiratory compromise • Infection near the puncture site • Space occupying lesion
  • 36. EQUIPMENT : • Spinal needle – Less than 1 yr: 1.5in – 1yr to middle childhood: 2.5in – Older children and adults: 3.5in • Three-way stopcock • Manometer • 4 specimen tubes • Local anesthesia • Drapes • Betadine
  • 37. PROCEDURE : • Performed with the patient in the lateral recumbent position. • A line connecting the posterior superior iliac crest will intersect the midline at approx. the L4 spinous process. • Spinal needles entering the subarachnoid space at this point are well below the termination of the spinal cord.
  • 38. • LP in older children may be performed from L2 to L3 interspace to the L5 to S1 interspace. • At birth, the cord ends at the level of L3. • LP in infant may be performed at the L4 to L5 or L5 to S1 interspace.
  • 39. • Position the patient: – Generally performed in the lateral decubitus position. – A pillow is placed under the head to keep it in the same plane as the spine. – Shoulders and hips are positioned. perpendicular with the table. – Lower back should be arched toward practitioner.
  • 40. a. Ligament flavum is a strong, elastic, yellow membrane covering the interlaminar space between the vertebrae. b. Interspinal ligaments join the inferior and superior borders of adjacent spinous processes. c. Supraspinal ligament connects the spinous processes
  • 41. • A topical anesthetic (e.g. EMLA cream) can be applied 30 to 60 minutes before performing the puncture to minimize pain on penetration. • Either a sitting or lateral decubitus position can be used . • Monitor the patient visually and with pulse oximetry for any signs of respiratory difficulty as a result of assumed position. • The subarachnoid space must be entered below the level of spinal cord termination. • The spine should be flexed maximally to increase spacing between spinous processes.
  • 42. • The patient’s back should be carefully prepared and draped using provided disinfecting solution and drapes. • Orient yourself anatomically and find the L4 spinous process at the level of iliac crests • Palpate a suitable interspace distal to this level. • Infiltrate 2% Lidocaine subcutaneously (without epinephrine to prevent cord infarction should it be introduced into the cord by accident) with a fine needle. • A field block can be applied injecting into and on either side of the interspinous ligaments. • Identify the two spinal processes in between which the needle will be introduced, penetrate the skin and slowly advance the tip of the needle at about 10 degrees cephalad (i.e. toward the patient’s umbilicus).
  • 43. • Remove the stylet and check for clear fluid will flow from the needle when the subarachnoid space has been penetrated. • The ligaments offer resistance to the needle, and a “pop” is often felt as they are penetrated. • Withdraw the needle leaving the tip in, recheck the landmarks and slowly progress the needle again. • Measure the opening pressure using the manometer by attaching it via a stopcock to the spinal needle. • Normal opening pressure ranges from 10 to 100 mm H2O in young children and 60 to 200 mm H2O after eight years of age
  • 44. • CSF volume of 1cc obtained in 3 tubes. • In the neonate, 2ml in total can be safely removed. • In an older child 3 to 6 ml can be sampled depending on the child’s size. • Tube 1 is used for determining protein and glucose • Tube 2 is used for microbiologic and cytologic studies • Tube 3 is for cell counts and serologic tests for syphilis
  • 45. COMPLICATIONS : • Herniation • Cardiorespiratory compromise • Pain • Headache (36.5%) • Bleeding • Infection • Subarachnoid epidermal cyst • CSF leakage
  • 47. INDICATIONS : • Diagnostic : - Idiopathic Thrombocytopenic Purpura - Aplastic Anemia - Leukemia - Megaloblastic Anemia - Infections e.g. Kala Azar - Storage disorders e.g. Gaucher’s disease - PUO - Myelofibrosis • Therapeutic : - Bone Marrow Transplantation
  • 48. CONTRAINDICATIONS : • Hemorrhagic disorders such as congenital coagulation factor deficiencies (eg, hemophilia), disseminated intravascular coagulation and concomitant use of anticoagulants. • Skin infection or recent radiation therapy at the sampling site. • Bone disorders such as osteomyelitis or osteogenesis imperfecta.
  • 49.
  • 50. PROCEDURE : • Obtain consent from a parent or guardian. • If the posterior iliac crest is the chosen site, patients are generally placed in the lateral decubitus position or the prone position • Sterilize the site with the sterile solution • Place a sterile drape over the site, and administer local anesthesia, letting it infiltrate the skin, soft tissues, and periosteum. • After local anesthesia has taken effect, make an incision through which the bone marrow aspiration needle can be introduced .
  • 51.
  • 52.
  • 53. • If a guard is present, should be removed before starting bone marrow aspiration, to ensure adequate depth of penetration.. • In general, the needle should be advanced at an angle completely perpendicular to the bony prominence of the iliac crest. • Once the needle passes through the cortex and enters the marrow cavity, it should stay in place without being held. • Once the periosteum has been penetrated, pressure is used to advance the needle through the cortex and rotate the needle in a semicircular motion, alternating clockwise and counterclockwise movements.
  • 54. • If the patient is in the lateral position, the hip may be stabilized with the other hand to get a better feel for the position and depth of the needle. • The thumb of this hand can be to mark the desired site and to prevent accidental repositioning of the needle. • A slight give will be felt, after which you will feel that the needle is fixed solidly within the bone. • Remove the stylet and aspirate approximately 1 ml of unadulterated bone marrow into a syringe. • Specimen is taken and is assessed for the presence of bony spicules.
  • 55. • If the specimen shows spicules, the specimen should be used to make smear slides immediately. • If spicules are sparse or are not present, a new sample should be obtained from a slightly different site. • The needle is left in place and sequential syringes are filled that have been prepared with heparin or other anticoagulants or preservatives, depending on the requirements for specific studies to withdraw samples for additional analysis. • Then remove the needle, either after reinserting the stylet or with the syringe attached.
  • 56. COMPLICATIONS : • Hemorrhage • Infection • Persistent pain at the marrow site • Retroperitoneal hematomas • Trauma to neighboring structures (e.g., lacerations of a branch of the gluteal artery) and soft tissues