Real-Time PCR Validation of GM Canola Detection Method
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Determination of a validation protocol, based on established European Union methods, for the detection of transgenic canola (Roundup Ready® - Monsanto) in seed samples using molecular techniques
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Real-Time PCR Validation of GM Canola Detection Method
- 1. Claudio Iannetta (s3154021) Supervisor: Saman Buddhadasa Senior Food Scientist Food Metrology National Measurement Institute
- 20. Project – Flow Chart ✔ ✔ ✔
- 39. Data Analysis The consistency of the results is assured if Coefficient of Variation is below 25%, which is the recommended value from EU Network of GMO Laboratories Sample A Sample B E ( FatA ) R 2 ( FatA ) 1 2.18 x 10 3 917.891 95% 99% 2 2.26 x 10 3 840.949 3 2.24 x 10 3 879.143 Mean 2.23 x 10 3 879.327 SD 41.63 38.47 CV 1.87% 4.37%
- 41. References
Editor's Notes
- Self introduction: Good morning/afternoon to everybody My name is Claudio Iannetta and I am a Master student in Biotechnology, with a major in Food Sc & Tech here at RMIT University My research project has been a collaboration between RMIT-University and NMI (National Measurement Institute), both placed in Melbourne. The supervision of the project has been guided by Dr. Saman Buddhadasa (NMI) and Dr Gregory Nugent (RMIT University).