6. Prefix & Suffix Prefix: GAATTC GCGGCCGC T TCTAGA G CTTCCG CGCCGGCG A AGATCT C Suffix: T ACTAGT A GCGGCCG CTGCAG A TGATCA T CGCCGGC GACGTC EcoRI XbaI SpeI PstI NotI Prefix cut with XbaI: ...GCT T^CTAG A G ...CGA A GATC^T C Suffix cut with SpeI: T A^CTAG T AGC... A T GATC^A TCG... SpeI + XbaI ...T A^CTAG A G... ...A T GATC^T C... http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix http://partsregistry.org/Help:Plasmid_backbones/Glossary