Meeting welcome and overview of Eukaryotic -Omics ressearch at UC Davis

1. #SMBEeuks
April 29 – May 2, 2013
UC Davis Conference Center

2. Logistics
• Wi-Fi
• Lunch/Dinner

4. Logistics
• Wi-Fi
• Lunch/Dinner
• Instructions for posters
• Instructions for talks
• Sponsor thanks
• Prize for travel award recipients

5. It all started in Florida…

6. 2011 Catalysis Meeting at NESCent

8. Future Goal:
Research
Coordination
Network

10. Building a Eukaryotic Community
• Relaxed meeting schedule
• Emphasis on speaker diversity
• Breakout groups on Thursday - outputs
• Get to know your neighbor – socialize!
• Tweet! (Profusely, if possible)

11. Photo Credit: J. Baldwin, M. Mundo-Ocampo

12. Biodiversity?
Phylogeography?
Environmental Impacts?

13. Extract Environmental DNA
Amplify rRNA
High-throughput
sequencing
Community analysis
Diverse marine community
EASY
EASY
EASY
VERY
Difficult!

14. http://phylosift.wordpress.com

15. Explicitly Phylogenetic Approaches
Aligned
environmental
sequences
Guide Tree
Evolutionary
Placement of
short reads

17. We provide:
• Support for Paired End (raw) Illumina data
• Marker gene data for
Bacteria, Archaea, Eukaryotes, Viruses
• Taxonomy assignments based on probability
distributions over a reference phylogeny
• Complement to existing tools – QIIME, etc.
– Inputs/outputs will be compatible for use with
other software tools

19. Great!
Not Bad
Getting Tricky…

20. Marine
Metagenome
Tree Placement
Sing Tree - Guppy

21. Acknowledgements
UC Davis
• Jonathan Eisen
• Aaron Darling
• Guillaume Jospin
• Dongying Wu
PhyloSift Software Development on Github:
https://github.com/gjospin/PhyloSift
Google Group for user support:
https://groups.google.com/d/forum/phylosift
Twitter: @PhyloSift