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Moringa oleifera – Household
 Alternative Coagulant for
     Water Treatment.

           Presented BY
          Majithiya H. M .
      Email: hmajithiya@gmail.com
Introduction
    Coagulation-flocculation followed by
     sedimentation, filtration and disinfection, often by
     chlorine, is used worldwide in the water treatment
     industry before the distribution of treated water to
     consumers (Peavy 1995).

    However recent studies have pointed out several
     serious drawbacks of using Aluminium salts.



Introduction    Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
                                                                                                    2
               Objectives                 & Methods   Data Interpretation                 Recommendations
Introduction about Moringa oleifera




M. oleifera pods            M.O shelled seed           Shelled seed                M.O Press Cake

•It tropical plant belonging to the family Moringaceae .
•Seeds are brown, and the kernels are white Crushed
whole seed or presscake remaining after oil extraction as a
coagulant for water and wastewater treatment.                                                                3
Introduction    Aim &        Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                  & Methods   Data Interpretation                 Recommendations
Purpose of study
    In situation where high concentrations of alum are
     needed, lime is also added to raise the pH of
     water, thereby facilitating the treatment.
    The cost of procuring these chemicals is increasing
     rapidly and most developing countries are finding it
     difficult to cope.
    Therefore, where cheaper alternatives can be
     found, to replace or supplement the conventional
     treatment chemicals, their use would be a welcome
     benefit for the poorer less developed countries.                                                       4
Introduction    Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                 & Methods   Data Interpretation                 Recommendations
Natural Coagulant Vs Inorganic
                     Coagulant
 Drawbacks of Alum                                                                             -
  Alzhemeiers disease and similar health related
   problems associated with residual aluminum in
   treated water.
  It produces large sludge volumes.

  It require pH Alkalinity adjustment.

  Low efficiency in coagulation of cold water.

  It is costly and require high foreign exchange.
                                                                                                            5
Introduction    Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                 & Methods   Data Interpretation                 Recommendations
Advantage of Moringa oleifera
 Coagulant over Alum
       It is natural, completely non-toxic.
       The M. Oleifera seed extract appears to have
        natural buffering capacity so, no pH alkalinity
        adjustments are required.
       Beside level of turbidity it reduces the level of
        microorganism in water.
       It is completely biodegradable.
       The volume of sludge produced is considerably
        less in case of Moringa than in case of alum.                                                       6
Introduction    Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                 & Methods   Data Interpretation                 Recommendations
Drawbacks of Moringa oleifera
    At present availability of seeds is a problem it
     require mass cultivation.
    The costs of the Shelled Seed powder of M.O
     will probably higher then the cost of alum at
     present.
    The water treated by M. oleifera produces odour
     after two days from treatment.
    Another disadvantage water extract increases
     Dissolved Organic Carbon in treated water.
                                                                                                            7
Introduction    Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                 & Methods   Data Interpretation                 Recommendations
Aim & Objectives
                                                 Aim
               Optimization of dosage Affecting Coagulation and
                      Flocculation of Turbid Water Using
                        Moringa oleifera Seed Extract

                                            Objectives
    To optimize coagulation dose, slow and rapid mixing
     parameters.
    To improve the extraction method of coagulation
     active component.
    To study Household Alternative Coagulant for
      Water Treatment.
                                                                                                             8
Introduction     Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
                Objectives                 & Methods   Data Interpretation                 Recommendations
Materials and Methods
 Experiment Design
    Entire study was divided into different stages. And
     same experiment is repeated for three coagulants
     viz. Moringa oleifera shelled blended water
     extract, Moringa oleifera shelled blended oil
     extracted water extract & Alum.
                                                                                                            9
Introduction    Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                 & Methods   Data Interpretation                 Recommendations
   A series of experiments was conducted on three
     synthetic raw water turbidities viz. 50, 150, and
     450 NTU.

    Residual turbidity was used as the parameter to
     judge the performance of the process.

 (According to Bureau of Indian Standards. IS 3025(PART 50): 2001)




                                                                                                            10
Introduction    Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                 & Methods   Data Interpretation                 Recommendations
Result of Seed Analysis
 Sr.     Sample Name            Parameters         Results        Test
 No                                                              Methods
  1                               Protein         36.90 %  AOAC
                                                           920.152
  2                                  Fat          37.25 % Ranganna
  3                           Carbohydrates 16.38 %              IS: 1656-
                                                                   1997
  4    Moringa oleifera         Crude Fiber       12.85 %        SP-18 (P-
           Seeds                                                 IX) 1984
  5                               Moisture         6.41 %        Ranganna
  6                                Ash             3.06 %         AOAC
                                                                  940.26
National Agriculture Food Analysis And Research Institute, Pune. (10.08.2007)
                                                                                11
Preparation of Seed Extract



Moringa oleifera non-shelled & shelled seeds       M.O. Seed Powder




                                                                                12
       M.oleifera Seed Suspension         Extraction of the Active Ingredient
Preparation of “Synthetic Turbid Water”




          Kaolin Clay Bottle                       Stock Turbidity
                                                       Solution
 1. Five gram of Kaolin clay is mix 500 ml of distilled water.
 2. Mix clay sample is allowed to stand for 24 Hrs.
 3. The stock solution, turbidities varying from 50- 450 NTU prepared by
    serial dilution of stock solution.                                                                      13
Introduction    Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                 & Methods   Data Interpretation                 Recommendations
Equipment Used




 Jar Test Apparatus
                      14
Digital Lovibond Turbidity Meter




                                   Digital pH Meter




                                                      Rapid Mixer   15
Observation & Data Interpretation




                                                                                                            16
Introduction    Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                 & Methods   Data Interpretation                 Recommendations
Optimum Dosage of Moringa oleifera Shelled
                Blended.
                      OPTIMUM DOSAGE OF M.O. Shelled Blended w ater extract
                                   AT 50, 150, AND 450 NTU
                 60
    TURBIDITY
    RESIDUAL
    AVERAGE




                                                                                             50 NTU
      (NTU0




                 40
                                                                                31.27
                 20                     18.2             22.8                                150 NTU

                  0                                                                          450 NTU
                                                      *                   *
                      40    50   60 70* 80 110 120 0* 140 150 200 250 0* * 350
                                                   13                30
                                      DOSAGE OF M.O. Shelled Blended
                                           w ater extract (m g/l)




  Shelled blended Moringa oleifera was able to achieve 63.90 % turbidity
  removal at an optimum dosage of 70 mg/l for 50 NTU whilst for 150 and
  450 NTU turbidity the doses were 130 mg/l and 300 mg/l and % turbidity
  removal is 84.80 % and 93.10 % respectively.                                                               17
Introduction    Aim &        Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                  & Methods   Data Interpretation                 Recommendations
Optimum Dosage of Moringa oleifera
                    Shelled Blended oil extracted.
                 OPTIMUM DOSAGE OF M oringa oleifera Shelled Blended AT 50, 150, AND
                                             450 NTU
     TURBIDITY (NTU)




                   40
       RESIDUAL
       AVERAGE




                       30                                                                                50 NTU
                                                                25.13        26
                       20                                                                                150 NTU
                       10                          10.1                                                  450 NTU
                        0
                                10           50*          100**         150***          200
                              DOSAGE OF Moringa oleifera Shelled Blended
                                   oil extracted water extract (mg/l)


 Shelled blended oil extracted Moringa oleifera was able to achieve 78.7 %
 turbidity removal at an optimum dosage of 50 mg/l for 50 NTU whilst for
 150 and 450 NTU turbidity the doses were 100 mg/l and 150 mg/l and %
 turbidity removal is 83.24 % and 94.22 % respectively.                                                                 18
Introduction            Aim &        Methodology    Materials      Observations &       Conclusions    Suggestions &
                       Objectives                   & Methods     Data Interpretation                 Recommendations
Optimum Dosage of Alum
                                      OPTIMUM DOSAGE OF ALUM FOR 50, 150, AND 450 NTU
     AVERAGE RESIDUAL
      TURBIDITY (NTU)




                        20

                        15                                                                                50 NTU
                        10                                                                                150 NTU
                                                                                   7.2
                         5                                              4.73                              450 NTU
                                                              3.77
                         0
                               5         10          20    30*       40**      50***       60
                                                DOSAGE OF ALUM (mg/l)




    Alum was able to achieve 92.47 % turbidity removal at an optimum
    dosage of 30 mg/l for 50 NTU whilst for 150 and 450 NTU turbidity the
    doses were 40 mg/l and 50 mg/l and % turbidity removal is 96.24 %
    and 98.40% respectively.
                                                                                                                         19
Introduction              Aim &        Methodology    Materials    Observations &        Conclusions    Suggestions &
                         Objectives                   & Methods   Data Interpretation                  Recommendations
Summary of All Results

Coagulants
                                         M.O. Shelled Blended
               M.O. Shelled Blended
                                          oil Extracted water             Alum
(Concentrati      water extract
                                                extract
on 1 %)
 Synthetic
  Kaolin       Optimum         %         Optimum         %         Optimum        %
  Water         Dosage     Reduction      Dosage     Reduction      Dosage    Reduction
 Turbidity       mg/l     in turbidity     mg/l     in turbidity     mg/l    in turbidity
  (NTU)
    50           70          63.6          50          78.7          30          92.84
    150         130          84.8         100         83.24          40          96.84
    450         300          93.1         150         94.22          50          98.40




                                                                                    20
Conclusions
   Moringa oleifera seeds present a viable alternative to alum.
   Increase dosage of Moringa oleifera lead to decrease
    turbidity upto the optimum dosage after which the residual
    turbidity increases due to floc restabilization Increasing
    initial turbidity, optimum dose is subsequently found to
    increase.
   Requirement dose for Moringa oleifera for both Shelled
    blended and shelled blended oil extracted was higher than
    alum all over the turbidities range from 50- 450 NTU.



                                                                                                            21
Introduction    Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                 & Methods   Data Interpretation                 Recommendations
   The oil content in the seed will form an
    emulsion or film coating which may inhibit
    the contact with the surface of reaction and
    thus reduce floc formation. Shelled blend oil
    extracted Moringa oleifera has been found
    to be more effective than the shelled blended
    seeds as a primary coagulant for turbid
    water.
   It is easy to prepare the suspension and can
    be use as household water treatment.
   M. oleifera seed contains 40 % oil by weight & hence
     after oil extraction waste product “presscake” contain
     active component affecting coagulation in water
     treatment, therefore farmers can promote for cultivation.

    Using M. oleifera as a replacement coagulant for
     proprietary coagulants meets the meets the needs for
     water technology in developing countries which is simple
     to use, robust and cheap to both install and maintain
     sustainable as household coagulant.
                                                                                                            23
Introduction    Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                 & Methods   Data Interpretation                 Recommendations
   Moringa oleifera is a consumer friendly and
     environment friendly low alternative for rural
     areas or small scale water treatment plant. M.
     oleifera is a renewable resource can be grown
     on large scale.

    Research should therefore continue in this area
     so as to apply it to small and large scale water
     treatment in tropical development countries.

                                                                                                            24
Introduction    Aim &       Methodology   Materials    Observations &       Conclusions    Suggestions &
               Objectives                 & Methods   Data Interpretation                 Recommendations
Thank You !!!

        Save Environment
           Save Yourself…

                            25

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Moringa oleifera as alternative coagulant

  • 1. Moringa oleifera – Household Alternative Coagulant for Water Treatment. Presented BY Majithiya H. M . Email: hmajithiya@gmail.com
  • 2. Introduction  Coagulation-flocculation followed by sedimentation, filtration and disinfection, often by chlorine, is used worldwide in the water treatment industry before the distribution of treated water to consumers (Peavy 1995).  However recent studies have pointed out several serious drawbacks of using Aluminium salts. Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & 2 Objectives & Methods Data Interpretation Recommendations
  • 3. Introduction about Moringa oleifera M. oleifera pods M.O shelled seed Shelled seed M.O Press Cake •It tropical plant belonging to the family Moringaceae . •Seeds are brown, and the kernels are white Crushed whole seed or presscake remaining after oil extraction as a coagulant for water and wastewater treatment. 3 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 4. Purpose of study  In situation where high concentrations of alum are needed, lime is also added to raise the pH of water, thereby facilitating the treatment.  The cost of procuring these chemicals is increasing rapidly and most developing countries are finding it difficult to cope.  Therefore, where cheaper alternatives can be found, to replace or supplement the conventional treatment chemicals, their use would be a welcome benefit for the poorer less developed countries. 4 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 5. Natural Coagulant Vs Inorganic Coagulant Drawbacks of Alum -  Alzhemeiers disease and similar health related problems associated with residual aluminum in treated water.  It produces large sludge volumes.  It require pH Alkalinity adjustment.  Low efficiency in coagulation of cold water.  It is costly and require high foreign exchange. 5 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 6. Advantage of Moringa oleifera Coagulant over Alum  It is natural, completely non-toxic.  The M. Oleifera seed extract appears to have natural buffering capacity so, no pH alkalinity adjustments are required.  Beside level of turbidity it reduces the level of microorganism in water.  It is completely biodegradable.  The volume of sludge produced is considerably less in case of Moringa than in case of alum. 6 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 7. Drawbacks of Moringa oleifera  At present availability of seeds is a problem it require mass cultivation.  The costs of the Shelled Seed powder of M.O will probably higher then the cost of alum at present.  The water treated by M. oleifera produces odour after two days from treatment.  Another disadvantage water extract increases Dissolved Organic Carbon in treated water. 7 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 8. Aim & Objectives Aim Optimization of dosage Affecting Coagulation and Flocculation of Turbid Water Using Moringa oleifera Seed Extract Objectives  To optimize coagulation dose, slow and rapid mixing parameters.  To improve the extraction method of coagulation active component.  To study Household Alternative Coagulant for Water Treatment. 8 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 9. Materials and Methods Experiment Design Entire study was divided into different stages. And same experiment is repeated for three coagulants viz. Moringa oleifera shelled blended water extract, Moringa oleifera shelled blended oil extracted water extract & Alum. 9 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 10. A series of experiments was conducted on three synthetic raw water turbidities viz. 50, 150, and 450 NTU.  Residual turbidity was used as the parameter to judge the performance of the process. (According to Bureau of Indian Standards. IS 3025(PART 50): 2001) 10 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 11. Result of Seed Analysis Sr. Sample Name Parameters Results Test No Methods 1 Protein 36.90 % AOAC 920.152 2 Fat 37.25 % Ranganna 3 Carbohydrates 16.38 % IS: 1656- 1997 4 Moringa oleifera Crude Fiber 12.85 % SP-18 (P- Seeds IX) 1984 5 Moisture 6.41 % Ranganna 6 Ash 3.06 % AOAC 940.26 National Agriculture Food Analysis And Research Institute, Pune. (10.08.2007) 11
  • 12. Preparation of Seed Extract Moringa oleifera non-shelled & shelled seeds M.O. Seed Powder 12 M.oleifera Seed Suspension Extraction of the Active Ingredient
  • 13. Preparation of “Synthetic Turbid Water” Kaolin Clay Bottle Stock Turbidity Solution 1. Five gram of Kaolin clay is mix 500 ml of distilled water. 2. Mix clay sample is allowed to stand for 24 Hrs. 3. The stock solution, turbidities varying from 50- 450 NTU prepared by serial dilution of stock solution. 13 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 14. Equipment Used Jar Test Apparatus 14
  • 15. Digital Lovibond Turbidity Meter Digital pH Meter Rapid Mixer 15
  • 16. Observation & Data Interpretation 16 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 17. Optimum Dosage of Moringa oleifera Shelled Blended. OPTIMUM DOSAGE OF M.O. Shelled Blended w ater extract AT 50, 150, AND 450 NTU 60 TURBIDITY RESIDUAL AVERAGE 50 NTU (NTU0 40 31.27 20 18.2 22.8 150 NTU 0 450 NTU * * 40 50 60 70* 80 110 120 0* 140 150 200 250 0* * 350 13 30 DOSAGE OF M.O. Shelled Blended w ater extract (m g/l) Shelled blended Moringa oleifera was able to achieve 63.90 % turbidity removal at an optimum dosage of 70 mg/l for 50 NTU whilst for 150 and 450 NTU turbidity the doses were 130 mg/l and 300 mg/l and % turbidity removal is 84.80 % and 93.10 % respectively. 17 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 18. Optimum Dosage of Moringa oleifera Shelled Blended oil extracted. OPTIMUM DOSAGE OF M oringa oleifera Shelled Blended AT 50, 150, AND 450 NTU TURBIDITY (NTU) 40 RESIDUAL AVERAGE 30 50 NTU 25.13 26 20 150 NTU 10 10.1 450 NTU 0 10 50* 100** 150*** 200 DOSAGE OF Moringa oleifera Shelled Blended oil extracted water extract (mg/l) Shelled blended oil extracted Moringa oleifera was able to achieve 78.7 % turbidity removal at an optimum dosage of 50 mg/l for 50 NTU whilst for 150 and 450 NTU turbidity the doses were 100 mg/l and 150 mg/l and % turbidity removal is 83.24 % and 94.22 % respectively. 18 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 19. Optimum Dosage of Alum OPTIMUM DOSAGE OF ALUM FOR 50, 150, AND 450 NTU AVERAGE RESIDUAL TURBIDITY (NTU) 20 15 50 NTU 10 150 NTU 7.2 5 4.73 450 NTU 3.77 0 5 10 20 30* 40** 50*** 60 DOSAGE OF ALUM (mg/l) Alum was able to achieve 92.47 % turbidity removal at an optimum dosage of 30 mg/l for 50 NTU whilst for 150 and 450 NTU turbidity the doses were 40 mg/l and 50 mg/l and % turbidity removal is 96.24 % and 98.40% respectively. 19 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 20. Summary of All Results Coagulants M.O. Shelled Blended M.O. Shelled Blended oil Extracted water Alum (Concentrati water extract extract on 1 %) Synthetic Kaolin Optimum % Optimum % Optimum % Water Dosage Reduction Dosage Reduction Dosage Reduction Turbidity mg/l in turbidity mg/l in turbidity mg/l in turbidity (NTU) 50 70 63.6 50 78.7 30 92.84 150 130 84.8 100 83.24 40 96.84 450 300 93.1 150 94.22 50 98.40 20
  • 21. Conclusions  Moringa oleifera seeds present a viable alternative to alum.  Increase dosage of Moringa oleifera lead to decrease turbidity upto the optimum dosage after which the residual turbidity increases due to floc restabilization Increasing initial turbidity, optimum dose is subsequently found to increase.  Requirement dose for Moringa oleifera for both Shelled blended and shelled blended oil extracted was higher than alum all over the turbidities range from 50- 450 NTU. 21 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 22. The oil content in the seed will form an emulsion or film coating which may inhibit the contact with the surface of reaction and thus reduce floc formation. Shelled blend oil extracted Moringa oleifera has been found to be more effective than the shelled blended seeds as a primary coagulant for turbid water.  It is easy to prepare the suspension and can be use as household water treatment.
  • 23. M. oleifera seed contains 40 % oil by weight & hence after oil extraction waste product “presscake” contain active component affecting coagulation in water treatment, therefore farmers can promote for cultivation.  Using M. oleifera as a replacement coagulant for proprietary coagulants meets the meets the needs for water technology in developing countries which is simple to use, robust and cheap to both install and maintain sustainable as household coagulant. 23 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 24. Moringa oleifera is a consumer friendly and environment friendly low alternative for rural areas or small scale water treatment plant. M. oleifera is a renewable resource can be grown on large scale.  Research should therefore continue in this area so as to apply it to small and large scale water treatment in tropical development countries. 24 Introduction Aim & Methodology Materials Observations & Conclusions Suggestions & Objectives & Methods Data Interpretation Recommendations
  • 25. Thank You !!! Save Environment Save Yourself… 25