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When Specimens Arrive……,[object Object],Check and Ensure:,[object Object],patient's identification on specimen tallies with that on its' request form (also known as requisition form) and the despatch form. ,[object Object],Each case is assigned with a unique accession number and labelled clearly on the request forms as well as the specimens before processing.,[object Object],For Conventional Slides  CY-XXXX,[object Object],For Liquid Based Preparation  TP-XXXX,[object Object]
Conventional Smears,[object Object],Specimen is smeared onto a microscopic glass slide, spray fixed with alchohol, and sent to lab. ,[object Object],Receive and stain the slides (using the Leica XL auto-stainer).,[object Object],Manual mount slides before despatching them out to the cytotechnologists on screening duties (also known as screeners),[object Object],who will screen the slides for any abnormalities etc. ,[object Object]
Leica XL auto-stainer,[object Object],Taken from:,[object Object],http://medequipsource.com/images/Leica%20Autostainer%20XL.jpg,[object Object]
Liquid Based Preparation,[object Object],Patient's gynaecological sample is collected by the clinician using a cervical sampling brush, immersed and rinsed into a vial containing PreservCytsolution.,[object Object],PreservCyt Solution contains methanol, which enhances cell preservation and nuclear morphology.,[object Object],Specimen vials will be sent to the lab. After samples are received in the lab, processing is done using the ThinPrep 2000 processor. ,[object Object],If samples received are too mucoid or bloody, digestion must be done before running the samples using the processor.,[object Object]
ThinPrep 2000 Processor,[object Object],Taken from:,[object Object],http://websites.labx.com/rankin/pics/50949.jpg,[object Object]
Liquid Based Preparation ThinPrep 2000,[object Object],Make use of mechanical, pneumatic and fluidic principles for dispersion, cell collection, and cell transfer.,[object Object],Accessories:,[object Object],Specimen Vial, ThinPrep Microscopic Glass Slide, Fixative Vial, Transcyt membrane filter, filter cap.,[object Object],The specimen vial, fixative vial and the microscopic glass slide (which is a special coated glass slide for preparation of ThinPrep samples) is placed into the machine.TheTranscyt membrane filter is fixed onto the filter cap before being placed into the machine.,[object Object]
Left: Brush for specimen collectionRight: Specimen Vial containing PreservCyt Solution,[object Object],Taken from:,[object Object],http://www.imvs.sa.gov.au/tissuepath/graphics/cervex_thinprep.jpg,[object Object]
Left: Conventional Smear SlideRight: ThinPrep Slide (specimen collected at the centre circle area),[object Object],Taken from:,[object Object],http://cytologystuff.com/indexppt.htm?../powerpoint/morph1.htm,[object Object]
Liquid Based Preparation ThinPrep 2000,[object Object],Process: Fluid level detection  Dispersion  Filter wetting  Cell Collection  Waste Clearing Bubble Point Cell transfer.,[object Object],The filter assembly (filter cap + Transcytfilter) will be lowered; specimen vial raised towards the assembly. ,[object Object],When the membrane filter detects/makes contact with the fluid in the specimen vial  check if level is satisfactory before carrying on the process. ,[object Object],If level of fluid is not satisfactory, it will halt the program.,[object Object]
Liquid Based Preparation ThinPrep 2000,[object Object],Dispersion system activated; rotates the Transcytfilter assembly within the specimen(cell suspension)  creating shear forces in the fluid that are strong enough to separate randomly joined material and disperse mucus.,[object Object],Negative pressure will be applied to aid in the drawing of a small amount of fluid through the Transcytfilter to wet it.,[object Object]
Liquid Based Preparation ThinPrep 2000,[object Object],System will gently blow out the liquid in the filter to clear any cellular material from the filter menbrane surface,[object Object],[object Object],Pneumatic system will apply positive and negative pressure to the filter in a series of pulses draw the specimen through the filter membrane and collect the suspended cellular material onto the membrane surface. ,[object Object],[object Object],[object Object]
Liquid Based Preparation ThinPrep 2000,[object Object],The slide handler move the slide into contact with the transcytfilter  natural adhesion property of the cells and electrochemical charge of the glass slide promote the transfer of cells from the filter membrane onto the slide.,[object Object],Cells have a higher affinity for the glass slide than the membrane.,[object Object],Slight positive pressure may be introduced to aid in the process.,[object Object]
Liquid Based Preparation ThinPrep 2000,[object Object], Slide will then be dropped into the fixative vial  PAP stained using auto-stainer (just like conventional smears)  manual mount and dispatched to screeners for screening.,[object Object],Fixative vial contains 95% ethanol, which aid in fixing the cells onto the slides, and prevent cell drop (which may occur as slides are not necessarily immediately stained.),[object Object]
Slides placed (with label end facing down) here.,[object Object],Fixative vial placed here.,[object Object],Filter Cap,[object Object],Inside the processor,[object Object],Taken from:,[object Object],http://cyto.igabinet.pl/data/user_files/image/TP%20Processor%202.JPG,[object Object],Filter Assembly,[object Object],Transcyt Filter,[object Object],Specimen vial placed here.,[object Object]
ThinPrep Process (simplified diagram),[object Object],Taken from:,[object Object],http://cytologystuff.com/indexppt.htm?../powerpoint/morph1.htm,[object Object]
PAP staining,[object Object],Papanicolaou Staining,[object Object],Show the differences in cellular morphology, maturity and metabolic activity.,[object Object],Fixation  nuclear staining  cytoplasmic staining  clearing,[object Object],Stains used:,[object Object],Haematoxylin: Nuclear stain to demonstrate the nuclear chromatin and nuclear membrane.,[object Object],Orange G: Cytoplasmic stain use to demonstrate keratin of squamous cells.,[object Object],Modified Eosin Azure: stains various cellular components.,[object Object]
PAP staining protocol,[object Object]
PAP staining protocol (continue),[object Object]
SIP slides on CYTO - gynae

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SIP slides on CYTO - gynae

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