The Future Roadmap for the Composable Data Stack - Wes McKinney - Data Counci...
Presentación leandro vetcher
1. Green Pacific Biologicals
Junio 2012
Leandro Vetcher – CEO
lvetcher@greenpacificbio.com
@lvetcher
Green Pacific Biologicals - Not for distribution
2. GPB: Plataforma de ingenieria genetica
GPB
Stable, stronger More efficient Access to the
gene expression engineering entire cell
other
0
50
transformants
50
% expressing % expressing
transformants other GPB
0
Only company with rapid and stable
eukaryotic algae nuclear genetic engineering
Green Pacific Biologicals - PROPRIETARY and CONFIDENTIAL - Not for distribution 2
3. GPB: Soluciones para biocombustibles
Increase carbon
fixa on
Implement
efficient
Maximize oil secre on
carbon flux
into oil
Green Pacific Biologicals - PROPRIETARY and CONFIDENTIAL - Not for distribution 3
4. Expresión génica nuclear mas fuerte
GFP expression by Western blotting Accumulation of GFP by confocal microscopy
GFP protein Cell extract
Wild-type
stds (ng) WT GPB
GPB Strains
4
5. Avg. fluorescence
per cell (au)
Avg. fluorescence
per cell (au)
Expresión génica nuclear estable
5
6. Mas transformantes expresando transgenes
More rapid and efficient
engineering
Green Pacific Biologicals - PROPRIETARY and CONFIDENTIAL - Not for distribution 6
7. RNAi funcional- ingeniería metabólica
Genetic RNAi using GPB platform better than
traditional mutant
Starch lower Oil higher Growth normal
7
9. Secreción de aceites no-toxica
Non-toxic mechanism
Secreted LDs spontaneously separates, sidestepping
harvesting/extraction
Works by interactions between proteins on LDs and cell membrane
or other cellular components
Platform makes GPB uniquely positioned to implement oil secretion
in algae
9
10. Beneficios de secreción de aceites no-toxica
BASE CASE: GPB CASE:
• Milk harvested by killing cow • Milk harvested through secretion and cow returned to pasture
• Majority of energy Making cow • Majority of energy (grass) goes into making milk
• If cows are milked daily, there is no benefit to having cows that can
store more than one day’s worth of milk
MILK + MILK
?
11. Modelado Tecno-económico
Oil productivity model + operational & capital costs
• Physics: energy balanced,
reasonable energy inputs
•Biology: realistic growth and
enzymatic rates, cell densities, PSE
• Interaction between variables
(e.g., cell density affects total
energy captured)
• Pilot and commercial capex and
operational cost for PBRs and open
ponds (with Tryg Lundquist, PhD at
Cal Poly)
12. Modelado Tecno-económico: valor de GPB
Secretion
• Enables continuous process through recycling of cells after secretion
• Most benefit through higher TAG Productivity
• Elimination of several costly process steps in TAG recovery
Oil metabolic engineering
• Improved redirection of light energy into TAGs
• Very high oil content levels not necessary with oil secretion
Other
• Identify additional bioengineering solutions
• Prioritize R&D projects based on synergies and on relative impact in cost
Validation
We can reach $50/bbl with realistic performance metrics for each of our
genetic engineering solutions
13. Process Flow Diagram Algae Biofuel Facility
Base Case GPB Process
Make-up Water
Inoculation
Ponds Recirculation
Algae Biomass
Blowdown
Make-up Water
High Rate Algae Gravity
Settling Basin
Ponds Thickener
Algae Biomass
CO2 Digestate
Natural Gas
Anaerobic Solar Drying
Generator
CH4 Digester Beds
+
CO2
Residuals Algae Biomass
Unrefined Oil
Solvent Based
Flash Dryer
Oil-Extraction
Algae acts a catalyst that converts CO2 and sunlight into TAGs
efficiently, without being consumed in the process
Work with Tryg Lundquist, Assistant Professor at Cal Poly
14. Modelado Tecno-económico
Base Case 0% 50% 90%
500
Cost per barrel ($/bbl)
400
300
200
100
0
0 5 10 15 20 25 30 35 40 45 50 55 60
% Oil (w/w)
Early adoption of tecnoeconomic analysis provides:
Quantification of value of biofuel solutions
Guidance of R&D projects
Validation
Editor's Notes
Stable transgene expression Consistent transgene expression Much higher level of transgene expression More expressing transformants Access to all cellular compartmentsRNAi functional Strain health and stability #2 Algae has the ideal characteristics for an ideal renewable energy platform.satisfy increasing energy demands + mitigate effects of climate change+ protect increasingly scarce natural resource Minimize/avoid competition with arable land for agriculture High oil productivity per acre (10x-100X over plants) Use waste water and saline water, reducing competition for limited freshwater supplies Recycle carbon from CO2-rich flue emissions from power plants and other industrial emitters
GPB rnai- gives benefits of starch knockout/mutant- more oil- without drawbacks on growth rate.
A NOTE ON MILK STORAGE CAPACITY: There is a limit to how much milk a cow can produce in one day, since they can only process a fixed amount of grass (energetically limited) If cows are milked daily, there is no benefit to having cows that can store more than one day’s worth of milkBASE CASE: Once grown, cow produces milk which is harvested by killing cow Majority of available energy (grass) goes into making cow GPB CASE: Milk is harvested by secretion from cow (milking) and cows returned to pasture Roughly 100% available energy (grass) goes into making milk (once cow population is established)
