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חיזוי מצב הסוקצסיה בקרומי קרקע ביולוגים על ידי מדידות פיזיקליות ,  ביו - פיזיולוגיות ובשילוב עם שיטות מולקולריות  (DGGE 16S rRNA)  ו אנליזות של חומצות שומן פוספוליפידיות  ( PLFA ) בן - דוד א .  אריק 1 , צעדי אלי 1 ,  שר יוני 2 ,  צירקין רג ' ינה 2 ,  נג ' ידאת עלי 2 1 משאבי טבע ,  מרכז מחקר גילת ,  מינהל המחקר החקלאי . 2 המחלקה למיקרוביולוגיה והידרולוגיה סביבתית ,  המכונים לחקר המדבר ,  אוניברסיטת בן - גוריון בנגב .
שינויי אקלים ,[object Object],Image based on data from the  Intergovernmental Panel on Climate Change (IPCC)
[object Object],[object Object],שינויי אקלים
שינויי אקלים ,[object Object],[object Object],[object Object]
קרומי קרקע ביולוגים ,[object Object],[object Object]
קרומי קרקע ביולוגים ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
שלבים בהתפתחות קרום קרקע ביולוגי B. Boeken 10   mm 1 2 3 4 Loose soil Silt seal after wetting Cyanobacteria Crust with mosses and lichens
SEMI-ARID ARID HYPER-ARID BEER  SHEVA NIZZANA ANNUAL AVERAGE RAINFALL STUDY SITES
השערות המחקר ,[object Object],[object Object]
מטרות המחקר ,[object Object],[object Object]
שיטות המחקר ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
חומצות שומן פוספוליפידיות   (PLFA) ,[object Object],[object Object],[object Object],[object Object],חומצות שומן פוספוליפידיות  הינם המרכיב העיקרי בממברנה של מיקרואורגניזמים
אנליזת חומצות שומן פוספוליפידיות
אנליזת  DGGE DGGE  –  פרופיל וזהות של מינים דומיננטים באוכלוסייה
משתנים פיזיקלים משתנים ביופיזיקלים
Fig. 1. Total PLFA-based biomass (nmol g - 1  dry weight) for the three sites along the aridity gradient according to the method of  Franzmann  et al.  (1996) . ביומסה מיקרוביאלית   כללית  ( nmol PLFA g -1 )  לאורך גראדינט הגשם a a b
יחסים נבחרים בין קבוצות חומצות שומן יחס גרם שלילי /  גרם חיובי יחס חידקים / אוכלוסייה כללית שיעור השוני באוכלוסייה
Fig. 2. PCA ordination of relative PLFA abundance data (mol%) for the crust samples   from the three tested sites. (a) The PCA ordination plot showing average and standard   deviation of the coordinates of each point from each of the three sites. (b) The loading   plot of individual PLFA species. אורדינצית  PCA   של נתוני אחוזי חומצות השומן הפוספוליפידיות בקרומי קרקע  משלושת האתרים
16S rRNA Targeted DGGE Fingerprinting of Microbial Communities Dendrogram from multivariate cluster, Ward's cluster analysis of the DGGE banding patterns of the three sites. Denaturing Gradient Gel Electrophoresis (DGGE) banding pattern differences between the three sites. SEMI   ARID ARID HYPER ARID SEMIARID HYPER ARID ARID
Closest matches of excised and sequenced 16S rRNA-derived DGGE bands SEMI ARID ARID HYPER ARID
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Monitoring biological soil crust succession using molecular analyses

  • 1.  
  • 2. חיזוי מצב הסוקצסיה בקרומי קרקע ביולוגים על ידי מדידות פיזיקליות , ביו - פיזיולוגיות ובשילוב עם שיטות מולקולריות (DGGE 16S rRNA) ו אנליזות של חומצות שומן פוספוליפידיות ( PLFA ) בן - דוד א . אריק 1 , צעדי אלי 1 , שר יוני 2 , צירקין רג ' ינה 2 , נג ' ידאת עלי 2 1 משאבי טבע , מרכז מחקר גילת , מינהל המחקר החקלאי . 2 המחלקה למיקרוביולוגיה והידרולוגיה סביבתית , המכונים לחקר המדבר , אוניברסיטת בן - גוריון בנגב .
  • 3.
  • 4.
  • 5.
  • 6.
  • 7.
  • 8. שלבים בהתפתחות קרום קרקע ביולוגי B. Boeken 10 mm 1 2 3 4 Loose soil Silt seal after wetting Cyanobacteria Crust with mosses and lichens
  • 9. SEMI-ARID ARID HYPER-ARID BEER SHEVA NIZZANA ANNUAL AVERAGE RAINFALL STUDY SITES
  • 10.
  • 11.
  • 12.
  • 13.
  • 14. אנליזת חומצות שומן פוספוליפידיות
  • 15. אנליזת DGGE DGGE – פרופיל וזהות של מינים דומיננטים באוכלוסייה
  • 17. Fig. 1. Total PLFA-based biomass (nmol g - 1 dry weight) for the three sites along the aridity gradient according to the method of Franzmann et al. (1996) . ביומסה מיקרוביאלית כללית ( nmol PLFA g -1 ) לאורך גראדינט הגשם a a b
  • 18. יחסים נבחרים בין קבוצות חומצות שומן יחס גרם שלילי / גרם חיובי יחס חידקים / אוכלוסייה כללית שיעור השוני באוכלוסייה
  • 19. Fig. 2. PCA ordination of relative PLFA abundance data (mol%) for the crust samples from the three tested sites. (a) The PCA ordination plot showing average and standard deviation of the coordinates of each point from each of the three sites. (b) The loading plot of individual PLFA species. אורדינצית PCA של נתוני אחוזי חומצות השומן הפוספוליפידיות בקרומי קרקע משלושת האתרים
  • 20. 16S rRNA Targeted DGGE Fingerprinting of Microbial Communities Dendrogram from multivariate cluster, Ward's cluster analysis of the DGGE banding patterns of the three sites. Denaturing Gradient Gel Electrophoresis (DGGE) banding pattern differences between the three sites. SEMI ARID ARID HYPER ARID SEMIARID HYPER ARID ARID
  • 21. Closest matches of excised and sequenced 16S rRNA-derived DGGE bands SEMI ARID ARID HYPER ARID