The rodentPACK2 transmits data via radiotelemetry, offering a well-tolerated, humane alternative to tethered EEG. Direct inputs from the device to cortical and deep brain regions provide a high signal-to-noise ratio, facilitating the detection of EEG events. The EEG analyzer module, within ecgAUTO software, allows scientist to easily: generate FFT power spectrums with user defined frequency bands detect epilepsy seizures differentiate between four sleep stages and calculate global sleep score.

1. cliquer pour ajouter un titre
Epileptic seizure
detection
& sleep scoring
EEG telemetry
semi-invasive biopotential,
temperature & activity in rodents
www.emka.fr
www.emka.fr

2. software for CNS studies
rodentPACK2 EEG telemetry
A
B
C
D
E
sources of EEG/EMG data
epilepsy seizure detection
sleep scoring
rodentPACK2 for EEG telemetry
using third party files and telemetry
www.emka.fr

3. sources of EEG/EMG data
smart tools – swift services – sharp research
www.emka.fr
www.emka.fr

4. emka SOFTWARE
ACQUIRE & PROCESS DATA
• recorded by emka iox data acquisition software
• recorded from third party device with analog outputs
• converted from text file
• converted from third party acquisition software
• recorded directly from third party telemetry
www.emka.fr

5. B
epileptic seizure detection
smart tools – swift services – sharp research
www.emka.fr
www.emka.fr

6. EEG ANALYZER MODULE
within ecgAUTO software
Automatic software seizure detection is peformed
using combined analysis of various parameters
www.emka.fr

7. EPILEPSY SEIZURE DETECTION
within ecgAUTO software
• detection is based on a user defined multicriteria condition involving
1 to 4 parameters and associated thresholds
• among these parameters, some may be derived from FFT computations
• analysis is performed in successive epochs of adjustable length
and overlap percentage
www.emka.fr

8. EPILEPSY SEIZURE DETECTION
within ecgAUTO software
Example of detection settings:
• epochs of n seconds (typically 2 to 10)
• successive epochs overlap by x% (typically 0 to 50%)
• epoch is a candidate if its energy, or power within some frequency range
(typically 3Hz to 10hz) exceeds a threshold
• seizure consists of a minimum number of consecutive candidate epochs (typically 2 to 3)
www.emka.fr

9. EPILEPSY SEIZURE DETECTION
within ecgAUTO software
Epochs of 4s, with 50% overlap. Single threshold on total epoch energy.
Main data table shows epoch status and detected seizures
www.emka.fr

10. EPILEPSY SEIZURE DETECTION
www.emka.fr

11. EPILEPSY SEIZURE DETECTION
plot with individual spectrums shown along input EEG trace
www.emka.fr

12. EPILEPSY SEIZURE DETECTION
Detected seizures are visible:
• in main results table
• listed in a specific seizure table
for review and edition
www.emka.fr

13. SEIZURE SUMMARY TABLE
Seizure table shows:
• index
• time in file
• duration
• limits on trace
• individual epochs
www.emka.fr

14. www.emka.fr

15. SEIZURE SUMMARY TABLE
Seizure table other key features:
• display seizures listed per time, or increasing/decreasing duration
• summary per time frame (number and total duration of all seizures in successive 15mn
frames),
• status of criteria used to detect candidate epochs
www.emka.fr

16. EPILEPSY SEIZURE EDITION
• manual edition of seizures limits is possible
• invalid seizures can be deleted
• undetected seizures can be created
• valid seizures can be exported to text file
www.emka.fr

17. EPILEPSY SEIZURE EDITION
Automatically detected limits (in green)
Manual cursor to modify limits (blue & red arrows)
www.emka.fr

18. EPILEPSY SEIZURE EDITION
Manually added seizure shown in yellow on trace and in summary table
www.emka.fr

19. EPILEPSY SEIZURE INVALIDATION
Invalidated seizure in dotted red on trace, in pink in table
www.emka.fr

20. EPILEPSY SEIZURE EDITION TRACKING
All manual edition actions:
• shown in summary table and on data traces
• are recorded in data file
• can be reversed
www.emka.fr

21. EPILEPSY SEIZURE DATA EXPORT
Seizure text format table with date, time, seizure length
Full epoch per epoch table also available
__________
cpu-date
www.emka.fr
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
january 7, 2009
__________
__________
__________
__________
epilepsy table report
cpu-time
site-time
(hS01+)__EPL_epoch#
(hS01+)__EPL_lengthS
15:49:13.000
00:01:21.991
17
65.59
15:50:13.000
00:02:21.987
8
29.592
15:53:09.000
00:05:17.991
41
157.599
15:54:57.000
00:07:05.984
1
1.594
15:57:37.000
00:09:45.974
5
17.593
15:58:13.000
00:10:21.991
8
25.612
15:59:01.000
00:11:09.988
10
37.591
16:00:57.000
00:13:05.981
22
85.588
16:03:13.000
00:15:21.992
32
121.605
16:04:09.000
00:16:17.988
12
45.591
16:04:37.000
00:16:45.986
5
17.593
16:06:41.000
00:18:49.978
3
9.593
16:07:41.000
00:19:49.975
6
21.592
16:08:41.000
00:20:49.990
8
29.592
16:10:17.000
00:22:25.984
3
9.593
16:10:57.000
00:23:05.982
1
1.594
16:12:13.000
00:24:21.977
3
9.593
__________
__________
__________
__________
__________
_____18
end of report text file section

22. sleep scoring
smart tools – swift services – sharp research
www.emka.fr
www.emka.fr

23. POWER SPECTRUM ANALYSIS
EEG analyzer module withing ecgAUTO software
• power in up to 6 adjustable
frequency ranges
• absolute and relative
power values
• versatile scheduling of analysis,
epoch lengths, overlap factor,
windowing
www.emka.fr

24. SLEEP SCORING
From top: cortex, hippocampus,
muscle, sleep score, active wake,
quiet wake, REM sleep, SW sleep
• easily differentiate between
four sleep stages
• calculate global sleep scores
• FFT power spectrums
• epileptic seizures detection
• optional video for confirmation
of sleep score
www.emka.fr

25. SLEEP SCORING
Spectrum shown for each epoch - Automated and manual scores
www.emka.fr

26. SLEEP SCORING MODULE
• define thresholds applicable to any parameter from any input
• software applies logical rules to attribute 1 out of 4 sleep stages to each epoch
• trend graph displays parameters as well as sleep stages
• on the fly, adjust thresholds and see the effect on sleep scoring
• reassign scores, also using up to 15 custom labelled scores
www.emka.fr

27. smart tools – swift services – sharp research
www.emka.fr
www.emka.fr

28. SPECTRUM DATA EXPORT
Export FFT spectrum from each epoch
Possibility to group frequency data to limit number of data columns.
www.emka.fr

29. D
rodentPACK2
telemetry
smart tools – swift services – sharp research
www.emka.fr
www.emka.fr

30. TETHER-FREE EEG TELEMETRY
rodentPACK2 wireless, low cost, head-mounted EEG for rodents
Acquisition of EEG and other biopotential signals, as
well as activity/ positional data
Applications are varied, e.g. sleep and epilepsy studies,
ECG and EMG recording
www.emka.fr

31. TETHER-FREE EEG TELEMETRY
rodentPACK2 wireless, low cost, head-mounted EEG for rodents
• head-mounted biopotential telemetry for rodentstransmits data via radiotelemetry
• humane alternative to tethered EEG
• high signal to noise ratio
• replaceable battery for lifetime monitoring
www.emka.fr

32. TETHER-FREE EEG TELEMETRY
rodentPACK2 wireless, low cost, head-mounted EEG for rodents
• 4 biopotential signals (EEG, ECG, EMG) each
sampled at 1 kHz
• 3-axis acceleration, sampled at 50Hz on each axis
• global acceleration (computed from 3 axes), for
activity assessment
• 1 temperature, sampled at 1Hz
www.emka.fr

33. TETHER-FREE EEG TELEMETRY
rodentPACK2 wireless, low cost, head-mounted EEG for rodents
• electrodes implanted in the subject and linked to
a connector permanently glued onto the skull
• transmitter housing amplifiers, transmission electronics
and batteries, to be coupled to the head connector prior to experiment
• receiver to read transmitted data
www.emka.fr

34. FEATURES
Radio transmission range 5m or more
Transmission duration with same batteries: 150 hours
(cost of batteries: 3€ ($4US) / pair)
Group housing multiple animals in same cage
Up to 32 animals in same room with no required shielding
www.emka.fr

35. ADVANTAGES
Noise free, high quality EEG signal
EEG electrodes are rigidly welded on the head connector which is itself solidly linked to the
transmitter, therefore animal movements trigger no flexible wire movement and cause no artefact
3-axis acceleration allowing activity and posture assessment
Very low recurring cost
no transmitter refurbishment is ever necessary
batteries cost 3€ per recording
www.emka.fr

36. MORE ADVANTAGES
Low investment cost
To record simultaneously from 4 rats, your complete hardware investment is less than 13k€
($18kUS)
Even lower investment cost
By scheduling your recordings, you may further decrease cost.
For example, conducting a study on 16 subjects split in 4 groups of 4, only requires a total
hardware investment of 14k€ ($20kUS)
www.emka.fr

37. DATA TRACES
from rodentPACK2
5s of data from a rat - cortex, hippocampus, EMG, total acceleration
www.emka.fr

38. DATA TRACES
from rodentPACK2
50s of data from a rat - cortex, hippocampus, EMG, total acceleration
www.emka.fr

39. FREQUENTLY ASKED QUESTIONS
Does the device alter subject behaviour?
After an acclimation of under 24 hours, the rats ignore the device. They do not « touch » it nor
do they try to rub it on the cage sides
How long can the implanted electrodes and glued connector stay in place ?
Rats equipped with this types of electrodes and connectors have been routinely used for many
years in tethered systems
Rats used for our tests were implanted 12 months ago and are still in good health.
No special precautions were taken during this period to keep the skin-to-glue junction clean or
sterile.
www.emka.fr

40. third party data files
easyMATRIX to read
telemetry implant signal
www.emka.fr
smart tools – swift services – sharp research
www.emka.fr

41. ANALYZING DATA
FROM THIRD-PARTY SYSTEMS
emka TECHNOLOGIES analysis software can process data in the following formats:
• produced by emka iox acquisition software
• produced by DATAQUEST or PONEMAH software
• available as text files, for example from powerLab or Biopac
• available in format such as EDF, Ishne, others
www.emka.fr

42. easyMATRIX
Invasive telemetry acquisition with
easyMATRIX
A seamless solution to combine
measurements from telemetry implants
and the power of emka TECHNOLOGIES
software suite
www.emka.fr

43. easyMATRIX
• reads signal from any type of implant through any receiver
• contains its own ambient pressure transducer
• connects to computer through USB port (no need for an add-on A/D acquisition card)
• also runs when 2 to 4 receivers read signal from a single implant
• iox2 directly records fully calibrated data
• OpenART not required
www.emka.fr

44. easyMATRIX
• up to 16 receivers connect to a single easyMATRIX
• up to 8 analog signals connect to a single easyMATRIX
• perfect synchronization between telemetry implants and analog signals
•1 easyMATRIX manages 1 to 4 receivers per implant
• up to 4 receivers connect to a single easyMATRIX
• up to 4 easyMATRIX connect to a single computer
www.emka.fr

45. LEGACY & easyMATRIX SYSTEMS
www.emka.fr

46. SETUP EXAMPLE
4 animals with 4 receivers/animal

47. SETUP EXAMPLE
16 receivers connected to a single easyMATRIX

48. cliquer pour ajouter un titre
solutions for
lifesciences research
www.facebook.com/emkatechnologies
@emkaTECH
www.emka.fr
www.emka.fr